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Packaged batches of stem cells for regenerative medicine

The Spanish start-up Aglaris Cell is close to launching onto the market the world's first bioreactor that cultures cell in a fully automated way, without using toxic additives. The device has attracted interest from the University of Oxford and the pharmaceutical giant, Merk.

David Horna, a 33-year-old from Madrid and one of the co-founders of Aglaris Cell, whose offices are located in the Madrid Scientific Park (PCM), is in London this week to meet with investors to secure a second round of funding. Horna, alongside his two partners, Miquel Costa and Manuel A. Gonzlez de la Pea, created the company a little over two years ago with the aim of developing a device that would automate stem cell cultures thereby making advances in the production of 'live' medicines.

As David Horna explained, after four years of intensive research and development, the prototype called Aglaris Facer 1.0, patented in 2012 in Spain and in the process of obtaining its international patent, "is practically ready to be sold on the market."

The idea of developing this device came about when the partners, who worked in various fields of biotechnology, noticed that more and more industries were using cells and tissues in their production processes.

Fully automatic

"We saw that the way live medicines from stem cells were being produced was highly manual, and so we came up with the idea of designing and developing a cell culture bioreactor that could automate the entire process. We believe that the stem cell-based therapies sector is going to expand rapidly in the years to come and will become a very promising business," Horna stated.

He noted that there are other bioreactors on the market and some have been able to automate some of the stages in the process, "but ours is the first in the world to perform all the process stages in a fully automated way."

Until now, an additive called trypsin was usually used in this type of culture, however, trypsin is toxic for cells and removes part of the membrane's proteins. "It has been used up to now because there was no other alternative, but our technology does not need to use this product," Horna said.

"Instead, our development uses an iterative method of cell culture which enables us to completely automate and remove the need for human involvement in the cell separation and washing stages, without using any additives that increase the toxicity level. We have achieved this by using smart surfaces that make cell adhesion and de-adhesion possible depending on changes in the environment," the co-founder explained.

He also adds that "we are currently finalising the developments that also make it possible to use the same device to produce genetically-modified cell lines for cellular reprogramming and gene therapies." These advances build on the work Horna undertook for his thesis on smart surfaces at the Spanish National Centre for Cardiovascular Research (CNIC) and the Sarri Institute of Chemistry (IQS) which was published in the 'Advanced Healthcare Materials' journal.

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Packaged batches of stem cells for regenerative medicine

Stem Cell Therapy Market Worth $330 Million in 2020 – New Report by MarketsandMarkets

(PRWEB) May 26, 2014

The report Stem Cell Therapy Market by Treatment Mode (Autologous & Allogeneic), Therapeutic Applications (CNS, CVS, GIT, Wound Healing, Musculoskeletal, Eye, & Immune System) - Regulatory Landscape, Pipeline Analysis & Global Forecasts to 2020 analyzes and studies the major market drivers, restraints, opportunities, and challenges in North America, Asia-Pacific, Europe, and the Rest of the World (RoW).

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The global stem cell therapy market on the basis of the mode of treatment is segmented into allogeneic and autologous stem cell therapy. In addition, based on the therapeutic applications, the global stem cell therapy market is segmented into eye diseases, metabolic diseases, GIT diseases, musculoskeletal disorders, immune system diseases, CNS diseases, CVS diseases, wounds and injuries, and others.

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This report studies the global stem cell therapy market over the forecast period of 2015 to 2020.The market is poised to grow at a CAGR of 39.5% from 2015 to 2020, to reach $330million by 2020.

Download PDF brochure: http://www.marketsandmarkets.com/pdfdownload.asp?id=48.

A number of factors such as increasing funding from various government and private organizations, growing industry focus on stem cell research, and rising global awareness about stem cell therapies through various organizations are driving the growth of the global market. In addition, increasing funding for new stem cell lines, development of advanced genomic methods for stem cell analysis, and rising approvals of clinical trials for stem cell therapy are other factors that are propelling the growth of the market.

However, factors such as lack of required infrastructure, ethical issues related to embryonic stem cell, and difficulties related with the preservation of stem cell are restraining the growth of the market.

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Stem Cell Therapy Market Worth $330 Million in 2020 - New Report by MarketsandMarkets

Hematopoietic Stem Cell Transplantation: MS Breakthrough …

Multiple Sclerosis (MS) is a devastating disease for many who become afflicted with the diseases progressive form, often in the prime of their lives, with no cure and when the effectiveness of established MS treatment is so often disappointing. Discouragement can lead to pinning premature hope on unproven therapies that seem to hold promise.

For example, withchronic cerebrospinal venous insufficiency (CCSVI or CCVI) the so-called liberation therapy developed by Italian researcher Paolo Zamboni in 2008, Dr. Zamboni hypothesized that compromised flow of blood in the veins draining the central nervous system played a role as the cause or in the development of multiple sclerosis, and devised a procedure dubbed liberation therapy by the media that involves angioplasty (stenting) of key veins in an attempt to improve blood flow.

CCSVI was and still is greeted with skepticism within the medical community, and Wikipedias entry on the topic notes that Dr. Zambonis first published research was neither blinded nor did it have a comparison group, and that Dr. Zamboni also did not disclose his financial ties to Esaote, manufacturer of the ultrasound specifically used in CCSVI diagnosis.

The so-called liberation procedure has also been criticized for possibly resulting in serious complications and deaths while its benefits have not been proven, with the United States Food and Drug Administrations position being that it is not clear if CCSVI exists as a clinical entity, and that these treatments may cause more harm. Wikipedia notes that CCSVI research has been fast-tracked, but researchers thus far still have been unable to confirm whether CCSVI has a role in causing MS, consequently raising serious objection to the hypothesis of CCSVI originating multiple sclerosis. Research continues, and a 2013 study found that CCSVI is equally rare in people with and without MS, while narrowing of the cervical veins is equally common.

This writer is personally aware of specific cases in which CCSVI has seemed to result in substantial improvement in MS symptoms, and others where it proved ineffective. The jury, as they say, is still out, but a cautious approach seems to be prudent.

Likewise for another controversial new therapy being advocated for MS and other autoimmune diseases, known as high-dose immunosuppressive therapy with hematopoietic stem cell transplantation in MS and other autoimmune diseases, in which clinicians destroy the patients immune system with chemotherapy, and then reboot it with stem cells. Dr. Denis Federenko of the A.A. Maximov Hematology and Cell Therapy Department of the National Pirogov Medical Surgical Centre in Russia specializes in this treatment, pointing out that conventional therapies do not provide satisfactory control of multiple sclerosis; hormonal therapy helps to limit acute manifestations of the disease, but doesnt stop its progression; and Interferon therapy may help some patients, but in most cases it does not provide a stable long-term effect.

The Russian proponents of this approach to treating MS contend that chemotherapy eliminates the cause of the disease the autoimmune T-cells that are responsible for nerve tissue damage. Then the patient is transplanted with his/her own (autologous) stem cells, which were collected and frozen in advance. They say that over the past decade more than 700 patients have received this treatment, which they claim may stop progression of the disease in most patients and prevent further deterioration of their quality of life, and that MS patients will not need any maintenance therapy after transplantation. Claimed efficiency of high-dose immunosuppressive therapy with hematopoietic stem cell transplantation in multiple sclerosis approximates 75%-80%, and is most effective in young patients with rapidly progressing multiple sclerosis in its early stages, when the leading mechanism of the damage to the nervous system is autoimmune inflammation. In later stages of the disease, after irreversible damage is done, they caution that transplantations effect is limited.

In an opinion statement entitled Autologous hematopoietic stem cell transplantation as a treatment option for aggressive multiple sclerosis (Curr Treat Options Neurol. 2013 Jun;15(3):270-80. doi: 10.1007/s11940-013-0234-9) published in the journal Current Treatment Options in Neurology, N. Pfender, R. Saccardi, and R. Martin of the Department of Neurology at University Hospital Zurich in Zurich, Switzerland, observe that Despite the development of several injectable or oral treatments for relapsing-remitting multiple sclerosis (RRMS), it remains difficult to treat patients with aggressive disease, and many of these continue to develop severe disability.

The authors observe that over the last two decades, hematopoietic stem cell transplantation (aHSCT or HSCT) has been explored, and clinical studies have shown that aHSCT is able to completely halt disease activity in the majority of patients with aggressive RRMS, and that research on the mechanisms of action supports that aHSCT indeed leads to renewal of a healthy immune system.

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Hematopoietic Stem Cell Transplantation: MS Breakthrough ...

Stem cell development: Experts offer insight into basic mechanisms of stem cell differentiation

The world has great expectations that stem cell research one day will revolutionize medicine. But in order to exploit the potential of stem cells, we need to understand how their development is regulated. Now researchers from University of Southern Denmark offer new insight.

Stem cells are cells that are able to develop into different specialized cell types with specific functions in the body. In adult humans these cells play an important role in tissue regeneration. The potential to act as repair cells can be exploited for disease control of e.g. Parkinson's or diabetes, which are diseases caused by the death of specialized cells. By manipulating the stem cells, they can be directed to develop into various specialized cell types. This however, requires knowledge of the processes that regulate their development.

Now Danish researchers from University of Southern Denmark report a new discovery that provides valuable insight into basic mechanisms of stem cell differentiation. The discovery could lead to new ways of making stem cells develop into exactly the type of cells that a physician may need for treating a disease.

"We have discovered that proteins called transcription factors work together in a new and complex way to reprogram the DNA strand when a stem cell develops into a specific cell type. Until now we thought that only a few transcription factors were responsible for this reprogramming, but that is not the case," explain postdoc Rasmus Siersbaek, Professor Susanne Mandrup and ph.d. Atefeh Rabiee from Department of Biochemistry and Molecular Biology at the University of Southern Denmark.

"An incredibly complex and previously unknown interplay between transcription factors takes place at specific locations in the cell's DNA, which we call 'hotspots'. This interplay at 'hotspots' appears to be of great importance for the development of stem cells. In the future it will therefore be very important to explore these 'hotspots' and the interplay between transcription factors in these regions in order to better understand the mechanisms that control the development of stem cells," explains Rasmus Siersbaek.

"When we understand these mechanisms, we have much better tools to make a stem cell develop in the direction we wish," he says.

Siersbaek, Mandrup and their colleagues made the discovery while studying how stem cells develop into fat cells. The Mandrup research group is interested in this differentiation process, because fundamental understanding of this will allow researchers to manipulate fat cell formation.

"We know that there are two types of fat cells; brown and white. The white fat cells store fat, while brown fat cells actually increase combustion of fat. Brown fat cells are found in especially infants, but adults also have varying amounts of these cells.

"If we manage to find ways to make stem cells develop into brown rather than white fat cells, it may be possible to reduce the development of obesity. Our findings open new possibilities to do this by focusing on the specific sites on the DNA where proteins work together," the researchers explain.

Details of the study

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Stem cell development: Experts offer insight into basic mechanisms of stem cell differentiation

Southampton doctors perform ground breaking stem cell hip …

Doctors and researchers at theSouthampton University reported yesterday that they have completed the first hip surgery using a 3D printed implant and bone stem cell graft.

The 3D printed hip, made from titanium, was designed using the patient's CT scan and CAD CAM (computer aided design and computer aided manufacturing) technology, meaning it was designed to the patient's exact specifications and measurements.

The implant will provide a new socket for the ball of the femur bone to enter. Behind the implant and between the pelvis, doctors have inserted a graft containing bone stem cells.

The graft acts as a filler for the loss of bone. The patient's own bone marrow cells have been added to the graft to provide a source of bone stem cells to encourage bone regeneration behind and around the implant. Southampton doctors believe this is a game changer. Douglas Dunlop, Consultant Orthopaedic Surgeon, conducted the operation at Southampton General Hospital. He says: "The benefits to the patient through this pioneering procedure are numerous. The titanium used to make the hip is more durable and has been printed to match the patient's exact measurements -- this should improve fit and could recue the risk of having to have another surgery.

The graft used in this operation is made up of a bone scaffold that allows blood to flow through it. Stem cells from the bone marrow will attach to the material and grow new bone. This will support the 3D printed hip implant.

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Southampton doctors perform ground breaking stem cell hip ...

Success for fiance's online campaign to find stem cell donor

They were also encouraged to tweet out a photo of themselves shaking their face to show they had joined the register, and to nominate a friend to do the same. Her plea was retweeted thousands of times, and less than a month later, a match has been found.

Ms Robertson described the success of her campaign as "astounding", saying "A matching donor means that we can go ahead with Mike's bone marrow transplant. We know we have a rocky road ahead as a transplant is a serious procedure, but knowing there is a good match for Mike is a fantastic boost that we desperately needed.

"We are hugely grateful to the selfless person who has stepped forward to help Mike, and to everyone who has pledged to do the same for someone else, by joining the Anthony Nolan register."

The transplant is scheduled to take place in June, but the name of the donor has to remain confidential due to anonymity regulations.

Ann OLeary, Head of Register Development at Anthony Nolan, said: "We are absolutely delighted that a matching donor has been found for Mike... Its our goal to find a match for everyone who needs a transplant so its wonderful that Mikes friends and family are carrying on the fight against blood cancer and urging even more people to come forward and support the work of Anthony Nolan."

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Success for fiance's online campaign to find stem cell donor

Stem cell research on campus grows after $3M boost

Provided

Aparna Mahadevan, left, a graduate student in Natasza Kurpios' lab, discusses a research poster with Vimal Selvaraj, assistant professor in animal science, at the daylong Stem Cell Retreat May 17 on campus.

Unlocking knowledge about how organisms develop and repair, stem cell research holds great promise for future therapies for injuries and conditions, from infertility and Alzheimers to heart failure and cancer. As part of its mission to promote cross-campus interactions and enhance training in stem cell biology at Cornell, the Cornell Stem Cell Program (CSCP) held its second Stem Cell Retreat May 17 on campus.

About 85 members of the Cornell stem cell research community attended the event, which featured keynote speaker Dr. Lawrence Goldstein, director of the Stem Cell Program at University of California, San Diego, and the Howard Hughes Medical Institute, speaking on using stem cells to treat Alzheimers disease.

Stem cell research involves a wide breadth of disciplines, said Dr. Alexander Nikitin, professor of pathology at Cornells College of Veterinary Medicine and leader of CSCP. This retreat is one example of how weve been able to foster the communications and collaborations across fields necessary to more fully understand and harness the potential of stem cells.

More than 40 laboratories at Cornell are affiliated with CSCP. Its faculty is supported by more than $42 million in research funding devoted to projects with major stem cell and regenerative medicine components. Coordinating activities of investigators involved in stem cell research, the CSCP provides resources for stem-cell research, promotes cross-campus interactions, and enhances teaching and training in stem cell biology.

Recently CSCP researchers Professor John Schimenti and Nikitin received $3 million from the New York State Stem Cell Science program of the New York State Department of Health to support the Stem Cell Modeling and Phenotyping Core.

The core expands existing mammalian reprogramming and transgenesis facilities and adds new components, such as genomic editing, stem cell pathology and stem cell optical imaging. Thus it provides state-of-the art capabilities for scientists to generate stem cells, modify genes in highly specific ways, create transgenic research animal models for basic and clinical research, analyze pathology of these animal models with high diagnostic and microscopic resolution, and study individual stem cells in live animals or in man-made environments.

The core consists of the Stem Cell and Transgenics Core Facility (directed by Schimenti), the Core Stem Cell Pathology Unit (directed by Nikitin) and the Core Stem Cell Optical Imaging Unit (directed by Associate Professor Warren Zipfel). Their combined resources and services integrate advanced animal modeling and phenotyping, the study of how genes affect physical traits.

In the last year CSCP-affiliated faculty members have published 130 manuscripts, including 53 that resulted directly from travel awards and seed funding from CSCP, and were published in such renowned journals as Nature, Science, Developmental Cell and PNAS. In 2013 CSCP gave seven travel awards and two seed grants to spur research, and hosted six seminars by invited stem cell experts and nine Stem Cell Work in Progress meetings, at which investigators from labs from across campus gather to share current work.

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Stem cell research on campus grows after $3M boost

Stem Cells Market By Application (Regenerative Medicine), By Technology (Acquisition, Sub-Culture), By Product (Adult …

San Francisco, California (PRWEB) May 22, 2014

The global market for stem cells is expected to reach USD 170.15 billion by 2020, according to a new study by Grand View Research, Inc. Growing prevalence of chronic diseases such as cardiovascular and liver disease, diabetes and cancer coupled with the presence of high unmet medical needs in these disease segments is expected to drive market growth during the forecast period. Moreover, increasing government support pertaining to funding R&D initiatives and the growing demand for medical tourism and stem cell banking services is expected to boost the demand for stem cells over the next six years. The future of this market is expected to be driven by opportunities such as the growing global prevalence of neurodegenerative diseases, increasing demand for contract research outsourcing services and the substitution of animal tissues by stem cells in the

The stem cells technology market was valued at USD 12.88 billion in 2013 and is expected to grow at a CAGR of over 12.0% during the forecast period. This market was dominated by the cell acquisitions technology segment in terms of share in 2013 owing to the fact that this technology serves as the foremost step to process involving stem cells culture. The global stem cell acquisition technology market is expected to reach USD 10.88 billion by 2020, growing at a CAGR of over 14.0% over the next six years.

The report Stem Cells Market Analysis By Product (Adult Stem Cells, Human Embryonic Cells, Pluripotent Stem Cells), By Application (Regenerative Medicine, Drug Discovery and Development) And Segment Forecasts To 2020, is available now to Grand View Research customers at http://www.grandviewresearch.com/industry-analysis/stem-cells-market

Request Free Sample of this Report @ http://www.grandviewresearch.com/industry-analysis/stem-cells-market/request

Further key findings from the study suggest:

Browse All Biotechnology Market Reports @ http://www.grandviewresearch.com/industry/biotechnology

For the purpose of this study, Grand View Research has segmented the global stem cells market on the basis of product, application, technology and region:

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Global Polymethyl Methacrylate (PMMA) Market Expected to Reach USD 10.87 Billion by 2020 (https://www.grandviewresearch.com/industry-analysis/polymethyl-methacrylate-pmma-industry)

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Stem Cells Market By Application (Regenerative Medicine), By Technology (Acquisition, Sub-Culture), By Product (Adult ...

OHSU Scientist Pushes Forward With Stem Cell Research

Contributed By:

Dave Blanchard

OPB | May 22, 2014 12:06 p.m. | Updated: May 22, 2014 1:51 p.m.

An egg cell's nucleus is extracted by apipette.

OHSU

This March, Oregon Health & Science University (OHSU) created a new Center for Embryonic Cell and Gene Therapy. The facility will be focused in part on advancing the work of Shoukhrat Mitalipov, one of the worlds leading researchers on embryonic stem cells. Mitalipov has been working for years on two promising areas of stem cellscience.

The first research area is a gene therapy for women with diseases stored in DNA located in their mitochondria. Mitalipovs lab has developed a technique to extract the nucleus from a cell with damaged mitochondrial DNA, and implant it in a cell with healthy mitochondria. The process would allow most of the mothers DNA to be inherited by her child, without the risk of the mitochondrial diseases. Mitalipov hopes to begin clinical trials of the procedure, and the FDA is in the process of deciding whether to approve the technique soon. Some critics have ethical and medical concerns about creating an embryo with DNA from three differentpeople.

The second area, which has garnered even more attention, is the field of embryonic stem cell cloning. Last May, Mitalipovs lab became the first team to create human embryonic stem cells by cloning a breakthrough that was highlighted by Nature, Discover, Science, and National Geographic as one of the most significant science stories of the year. Now Miltalipovs lab is trying to figure out how to further that field ofresearch.

Well check in with Mitalipov to hear about his hopes for his areas of research, and where he thinks the future holds for stem cell science and genetherapy.

Rose E. Tucker Charitable Trust

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OHSU Scientist Pushes Forward With Stem Cell Research