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Gene-modified stem cell transplant protects patients from toxic side effects of chemotherapy, study suggests

By adminMay 11, 2012Stem Cell Medical Center

ScienceDaily (May 9, 2012) For the first time, scientists at Fred Hutchinson Cancer Research Center have transplanted brain cancer patients' own gene-modified blood stem cells in order to protect their bone marrow against the toxic side effects of chemotherapy. Initial results of the ongoing, small clinical trial of three patients with glioblastoma showed that two patients survived longer than predicted if they had not been given the transplants, and a third patient remains alive with no disease progression almost three years after treatment.

"We found that patients were able to tolerate the chemotherapy better and without negative side effects after transplantation of the gene-modified stem cells than patients in previous studies who received the same type of chemotherapy without a transplant of gene-modified stem cells," said Hans-Peter Kiem, M.D., senior and corresponding author of the study published in the May 9 issue of Science Translational Medicine.

Kiem, a member of the Clinical Research Division at the Hutchinson Center, said that a major barrier to effective use of chemotherapy to treat cancers like glioblastoma has been the toxicity of chemotherapy drugs to other organs, primarily bone marrow. This results in decreased blood cell counts, increased susceptibility to infections and other side effects. Discontinuing or delaying treatment or reducing the chemotherapy dose is generally required, but that often results in less effective treatment.

In the current study, Kiem and colleagues focused on patients with glioblastoma, an invariably fatal cancer. Many of these patients have a gene called MGMT (O6-methylguanine-DNA-methyltransferase) that is turned on because the promoter for this gene is unmethylated. MGMT is a DNA repair enzyme that counteracts the toxic effect of some chemotherapy agents like temozolomide. Patients with such an unmethylated promoter status have a particularly poor prognosis.

A drug called benzylguanine can block the MGMT gene and make tumor cells sensitive to chemotherapy again, but when given with chemotherapy, the toxic effects of this combination are too much for bone marrow cells, which results in marrow suppression.

By giving bone marrow stem cells P140K, which is a modified version of MGMT, those cells are protected from the toxic effects of benzylguanine and chemotherapy, while the tumor cells are still sensitive to chemotherapy. "P140K can repair the damage caused by chemotherapy and is impervious to the effects of benzylguanine," Kiem said.

"This therapy is analogous to firing at both tumor cells and bone marrow cells, but giving the bone marrow cells protective shields while the tumor cells are unshielded," said Jennifer Adair, Ph.D., who shares first authorship of the study with Brian Beard, Ph.D., both members of Kiem's lab.

The three patients in this study survived an average of 22 months after receiving transplants of their own circulating blood stem cells. One, an Alaskan man, remains alive 34 months after treatment. Median survival for patients with this type of high-risk glioblastoma without a transplant is just over a year.

"Glioblastoma remains one of the most devastating cancers with a median survival of only 12 to 15 months for patients with unmethylated MGMT," said Maciej Mrugala, M.D., the lead neuro oncologist for this study.

As many as 50 percent to 60 percent of glioblastoma patients harbor such chemotherapy-resistant tumors, which makes gene-modified stem cell transplant therapy applicable to a large number of these patients. In addition, there are also other brain tumors such as neuroblastoma or other solid tumors with MGMT-mediated chemo resistance that might benefit from this approach.

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Gene-modified stem cell transplant protects patients from toxic side effects of chemotherapy, study suggests

Osiris Therapeutics Reports First Quarter 2012 Financial Results

By adminMay 11, 2012Stem Cell Medical Center

COLUMBIA, Md.--(BUSINESS WIRE)--

Osiris Therapeutics, Inc. (NASDAQ: OSIR - News), the leading stem cell company focused on developing and commercializing products to treat medical conditions in inflammatory, cardiovascular, orthopedic, and wound healing markets, announced today its results for the first quarter ended March 31, 2012.

Highlights and Recent Developments

This was an excellent quarter all around for Osiris, said C. Randal Mills, Ph.D., President and Chief Executive Officer of Osiris Therapeutics. "It is gratifying to witness the solid growth of our Biosurgery products, driven by Osiris continued clinical success. I am particularly proud of how well our team is executing during our transition into a fully commercial entity, including our ability to tackle challenging reimbursement issues and expand our manufacturing and distribution capabilities."

First Quarter Financial Results

Biosurgery product revenue rose 49% from the previous quarter to $1.14 million. Total revenues were $4.6 million in the first quarter of 2012, which include the final amortization of license fees from our collaboration agreements. Total revenues in the first quarter of 2011 were $10.4 million, and consisted almost exclusively of amortized license fees. Net loss for the first quarter of 2012 was $1.3 million compared to net income of $4.0 million in the first quarter of 2011.

Research and development expenses for the first quarter of 2012 were $4.0 million, compared to $4.7 million incurred in the first quarter of 2011. General and administrative expenses were $1.5 million for the first quarter of 2012 compared to $1.7 million for the same period of the prior year. Net cash used in operations for the three months ended March 31, 2012 was $4.0 million. As of March 31, 2012, Osiris had $44.2 million of cash, receivables and short-term investments.

Webcast and Conference Call

A webcast and conference call to discuss the financial results is scheduled for today, May 11, 2012 at 9:00 a.m. ET. To access the webcast, visit the Investor Relations section of the company's website at http://investor.osiris.com/events.cfm. Alternatively, callers may participate in the conference call by dialing (877) 303-6133 (U.S. participants) or (970) 315-0493 (international participants).

A replay of the conference call will be available approximately two hours after the completion of the call through May 17, 2012. Callers can access the replay by dialing (855) 859-2056 (U.S. participants) or (404) 537-3406 (international participants). The audio replay confirmation code is 74747423. To access a replay of the webcast, visit the Investor Relations section of the company's website at http://investor.osiris.com/events.cfm.

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Osiris Therapeutics Reports First Quarter 2012 Financial Results

Four Ohio High School Seniors Awarded BioOhio STEM Scholarship

By adminMay 11, 2012Stem Cell Clinic

COLUMBUS, Ohio--(BUSINESS WIRE)--

Four Ohio high school seniors have been awarded the 2012 BioOhio Scholarship, designed to encourage and advance bioscience-related STEM education and career interests in Ohio. BioOhio received 345 applications this year, compared to 194 applications in 2011.

This years recipients of the non-renewable $1,250 scholarships are:

To be considered for a scholarship, the student must live in Ohio, be a senior or senior-equivalent in good standing, and enroll at an Ohio college with plans to pursue a bioscience-related degree. Application evaluations emphasized letters of recommendation and an essay describing their interest in the bioscience field and how they will prepare for a bioscience career.

In her essay, Emily Harker expressed her vision, Maybe I will help discover a way to make a useable beating heart with induced pluripotent stem cells, or maybe I will discover a polymer that can be used to improve joint replacements.

Lauren Chens experience as a Cleveland Clinic medical laboratory intern helped bring her future into focus. Through this opportunity, she wrote, I connected my childhood passion for science with an increasing interest in cell biology research.

Vivek Chhabrias career outlook received a boost from his internship at BioOhio-member EXCMR. I witnessed the energy of the field, he said in his essay. I saw its potential, and it made me realize that with our population constantly increasingas well as its longevityunderstanding the enigmas of the medical world is going to be more important than ever.

During her sophomore year, Natasha Williamson lost her mother to lung and brain cancer. Nathasha said that her mothers passing has inspired her to be the first in her family to earn a college degree. I want to study the field of science and hopefully, one day, find a cure to cancer, she wrote.

A non-profit organization charged with accelerating bioscience business, research, and education throughout the state, BioOhio established the BioOhio Scholarship Fund in October 2009 with $15,000. This investment has been divided equally over the first three years of the scholarship fund, with plans on sustaining and increasing the fund through private, tax-deductible donations. Since 2010 the scholarship fund has received more than $9,400 in private contributions, $6,000 of which came from Hinckley, Ohio-based Clinical RM and the companys Making a Difference Initiative.

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Four Ohio High School Seniors Awarded BioOhio STEM Scholarship

Julio C. Voltarelli, Pioneer in Cell Transplantation, Dies at 63

By adminMay 11, 2012Uncategorized

Dr. Julio C. Voltarelli, who made a significant impact in cell transplantation, dies at 63

Distinguished Brazilian professor pioneered bone marrow transplantation

Newswise Tampa, Fla. (May. 9th , 2012) Julio C. Voltarelli, MD, PhD, professor at the Ribeiro Preto School of Medicine at the University of So Paulo, Brazil, died March 21, 2012 at the age of 63. Dr. Voltarelli, who was on the editorial board of the Cell Transplantation journal, published by Cognizant Communication Corporation, and an important factor in the journals success, was a distinguished stem cell researcher and head of the bone marrow transplantation unit at the Ribeiro Preto School of Medicine.

Dr. Voltarelli had a significant impact on Brazilian stem cell transplantation science, said Dr. Maria C. O. Rodrigues, Dr. Voltarellis longtime colleague. He was driven to bring the benefits of the newest cellular therapies to those with ALS, MS and type 1 diabetes. His efforts and dedication will be greatly missed.

Dr. Voltarelli, a graduate of the Ribeiro Preto School of Medicine, served post-doctoral fellowships at the University of California San Francisco, the Fred Hutchinson Cancer Research Center in Seattle, and the Scripps Research Institute in San Diego. He returned to Brazil in 1992 and started a highly ranked bone marrow transplantation program at the Ribeiro Preto School of Medicine. In 2002, Dr. Voltarelli initiated the schools research efforts in stem cell transplantation for autoimmune diseases, later focusing on diabetes, graft-versus-host disease and sickle cell anemia.

At the time of his death, Dr. Voltarelli, in addition to serving as head of the bone marrow transplantation unit, also served as research coordinator for the Center for Cellular Therapy at the So Paulo Research Foundation and the National Institute of Science and Technology in Stem Cells and Cell Therapy. He was recently elected president of the Brazilian Society of Bone Marrow Transplantation.

His publications included the first books on stem cell transplantation and clinical immunology written in Portuguese. He also founded the Brazilian Society of Stem Cell Transplantation.

His colleagues in Brazil called his lifelong contributions priceless and remembered him for his leadership skills, vision, and sense of humor.

# The Coeditor-in-chiefs for CELL TRANSPLANTATION are at the Center for Neuropsychiatry, China Medical University Hospital, TaiChung, Taiwan, and the Diabetes Research Institute, University of Miami Miller School of Medicine. Contact, Shinn-Zong Lin, MD, PhD at shinnzong@yahoo.com.tw or Camillo Ricordi, MD at ricordi@miami.edu or David Eve, PhD at celltransplantation@gmail.com #

News release by Florida Science Communications http://www.sciencescribe.net

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Julio C. Voltarelli, Pioneer in Cell Transplantation, Dies at 63

Regenerative medicine: Could the ways animals regenerate hair and feathers help restore human fingers and toes?

By adminMay 11, 2012Uncategorized

ScienceDaily (May 10, 2012) This summer's action film, "The Amazing Spider-Man," is another match-up between the superhero and his nemesis the Lizard. Moviegoers and comic book fans alike will recall that the villain, AKA Dr. Curt Connors, was a surgeon who, after losing an arm, experimented with cell generation and reptilian DNA and was eventually able to grow back his missing limb.

The latest issue of the journal Physiology contains a review article that looks at possible routes that unlock cellular regeneration in general, and the principles by which hair and feathers regenerate themselves in particular.

The authors apply what is currently known about regenerative biology to the emerging field of regenerative medicine, which is being transformed from fantasy to reality.

Review Article

While the concept of regenerative medicine is relatively new, animals are well known to remake their hair and feathers regularly by normal regenerative physiological processes. In their review, the authors focus on (1) how extrafollicular environments can regulate hair and feather stem cell activities and (2) how different configurations of stem cells can shape organ forms in different body regions to fulfill changing physiological needs.

The review outlines previous research on the role of normal regeneration of hair and feathers throughout the lifespan of various birds and mammals. The researchers include what is currently known about the mechanism behind this re-growth, as well as what gaps still exist in the knowledge base and remain ripe for future research.

The review examines dozens of papers on normal "physiological regeneration" -- the re-growth that happens over the course of an animal's life and not in response to an injury. This regeneration takes place to accommodate different stages in an animal's life (e.g., replacing downy chick feathers with an adult chicken's, or replacing the fine facial hair of a young boy with the budding beard of an adolescent), or in response to various environmental conditions (e.g., cats shedding a thick winter coat in the summer heat but re-growing it when the seasons change again, or snowshoe hares switching from brown in the summer to white in the winter for camouflage).

These changes seem to respond both to internal cues such as physiology of the hair follicle itself, or external cues such as the environment, but the mechanisms behind these normal alterations are largely unknown. Stem cells inside the follicle prompt hair and feather regeneration, but researchers are still unsure how to guide those cells to form the shape, size, and orientation of these "skin appendages" so that controlled re-growth is possible. Additionally, scientists are still unsure how to re-grow hair on skin in people after severe injuries that lead to scar tissue.

Importance of the Findings

The reviewed studies suggest that while researchers are making headway in understanding how and why hair and feathers regenerate after normal loss or in response to different life stages, much still remains unknown. This missing knowledge could hold valuable clues to learning how to regenerate much more complicated and valuable structures after loss to injury, such as fingers and toes.

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Regenerative medicine: Could the ways animals regenerate hair and feathers help restore human fingers and toes?

Study Identifies Cell Subtypes For Potential Personalized Cellular Therapies

By adminMay 11, 2012Uncategorized

Connie K. Ho for RedOrbit.com

A new study by researchers at the University of California, Los Angeles (UCLA) has discovered two adult stem cell-like subpopulations in adult human skin.

The findings allow for further research to be done in the area of personalized medicine and patient-specific cellular therapies.

The study, using technology from Fibrocell Science, allowed the researchers to identify and confirm two types of cells in human skin cell cultures; the possible source of stem cell-like subpopulations from skin biopsies would be faster to perform, painless, and less invasive than current extractions from adipose tissues and bone marrow.

The research, featured in the inaugural issue of BioResearch Open Access, discusses two subtypes of cells. BioResearch Open Access is a bimonthly, peer-reviewed journal. It features scientific topics like biochemistry, bioengineering, gene therapy, genetics, microbiology, neuroscience, regenerative medicine, stem cells, systems biology, tissue engineering and biomaterials, and virology.

Being able to identify two sub-populations of rare, viable and functional cells that behave like stem cells from within the skin is an important finding because both cell types have the potential to be investigated for diverse clinical applications, commented Dr. James A. Bryne, lead author of the report.

Brynes research, first at Stanford University then at UCLA, focused on reprogramming beginnings of cells from animals and then humans. A graduate of Cambridge University, Bryne studied the intra- and inter-species of epigenetic reprogramming. His work also highlighted how primate embryonic stem cells could be derived from somatic cell nuclear transfers.

The study published in BioResearch Open Access confirmed previous research that identified a rare population of cells in adult human skin that had a marker called stage-specific embryonic antigen 3 (SSEA3). Bryne and his colleagues found that there was an increase in the amount of SSEA3 expressing cells after injury to the human skin. It showed that the SSEA3 biomarker could be used to help identify and isolate cells with tissue-regenerative traits.

Finding these rare adult stem cell-like subpopulations in human skin is an exciting discovery and provides the first step towards purifying and expanding these cells to clinically relevant numbers for application to a variety of potential personalized cellular therapies for osteoarthritis, bone loss, injury and/or damage to human skin as well as many other diseases, remarked Bryne, an Assistant Professor of Molecular and Medical Pharmacology at the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA.

Bryne and his team used Fibrocell technology to collect cells from skin samples, cultured the cells in the lab, and purified them by fluorescence-activated cell sorting (FACS). The FACS tagged suspended cells with fluorescent markers for undifferentiated stem cells. The researchers were able to separate the rare cell subpopulations from other kinds of cells.

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Study Identifies Cell Subtypes For Potential Personalized Cellular Therapies

New England Journal of Medicine Reports on Three Phase III REVLIMID® (lenalidomide) Trials in Patients with Newly …

By adminMay 11, 2012Uncategorized

BOUDRY, Switzerland--(BUSINESS WIRE)--

Celgene International Srl, a subsidiary of Celgene Corporation (NASDAQ: CELG - News), today announced that results from three phase III studies evaluating the use of continuous REVLIMID (lenalidomide) treatment in newly diagnosed multiple myeloma (MM) patients or maintenance treatment with lenalidomide following autologous stem cell transplant were published online in the May 10, 2012 edition of the New England Journal of Medicine. All three publications highlight the expanding body of clinical evidence supporting lenalidomide treatment in these areas.

Continuous Lenalidomide Therapy (non-transplant eligible population):

The first article highlights a Celgene-sponsored study of continuous lenalidomide treatment in elderly patients newly diagnosed with multiple myeloma.

Continuous Lenalidomide Treatment for Newly Diagnosed Multiple Myeloma (MM-015)

This double-blind, phase III, multicenter, randomized study conducted by Celgene compared melphalanprednisonelenalidomide induction followed by lenalidomide maintenance (MPR-R), with melphalanprednisonelenalidomide (MPR), or melphalanprednisone (MP) followed by placebo in 459 patients aged 65 years with newly-diagnosed myeloma who were not eligible for autologous stem-cell transplant.

http://www.nejm.org/doi/full/10.1056/NEJMoa1112704

Post-transplant maintenance

The two additional articles published in the edition highlighted cooperative group studies that evaluated the use of lenalidomide maintenance following autologous stem cell transplant (ASCT).

In each of the studies, one funded by the National Cancer Institute and conducted by the Cancer and Leukemia Group B (CALGB) and one by the Intergroupe Francophone du Myelome (IFM), maintenance treatment with lenalidomide following ASCT resulted in delayed time to disease progression or death compared to placebo.

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New England Journal of Medicine Reports on Three Phase III REVLIMID® (lenalidomide) Trials in Patients with Newly ...

Gene-modified stem cell transplant protects patients from toxic side effects of chemotherapy

By adminMay 11, 2012Stem Cell Treatment

Public release date: 9-May-2012 [ | E-mail | Share ]

Contact: Dean Forbes dforbes@fhcrc.org 206-667-2896 Fred Hutchinson Cancer Research Center

SEATTLE For the first time, scientists at Fred Hutchinson Cancer Research Center have transplanted brain cancer patients' own gene-modified blood stem cells in order to protect their bone marrow against the toxic side effects of chemotherapy. Initial results of the ongoing, small clinical trial of three patients with glioblastoma showed that two patients survived longer than predicted if they had not been given the transplants, and a third patient remains alive with no disease progression almost three years after treatment.

Angie the chimp undergoes revolutionary stem cell treatment | Video

By adminMay 11, 2012Stem Cell Treatment

Chimp receives stem cells 25-year-old chimp received treatments for a torn ...

Photo by Eric Hasert

ERIC HASERT/TREASURE COAST NEWSPAPERS Dr. Darrell Nazareth, of the Florida Veterinary League in Vero Beach (background) injects chimpanzee stem cells with the help of veterinarian Dr. Jocelyn Bezner, of Save the Chimps, into the knee of Angie, a 25-year-old female chimp suffering from a torn anterior cruciate ligament, at the Save The Chimps Sanctuary in Fort Pierce. The surgery was performed inside the sanctuary's mobile surgical unit, which involved extraction of fat and blood cells that were transported to the Florida Veterinary League in Vero Beach to extract approximately 2.3 billion stem cells, then returning to the chimp sanctuary to finish the one-day procedure. "It feels wonderful, I can't wait to see the results two to three weeks out," Nazareth said about performing the procedure.

Photo by Eric Hasert

ERIC HASERT/TREASURE COAST NEWSPAPERS Nicole Devlin, a laboratory technician at the Florida Veterinary League in Vero Beach, works on a procedure to remove stem cells from blood and fat removed from Angie, a female chimp at Save The Chimps Sanctuary in Fort Pierce. After the stem cells were isolated, they were transported back to the chimp sanctuary in Fort Pierce to be injected back into Angie.

FORT PIERCE A 25-year-old female chimpanzee at the Save-the-Chimps sanctuary in Fort Pierce may be able to run again, thanks to a revolutionary stem cell treatment performed on Wednesday.

Angie, one of the 271 chimpanzees that live at the 150-acre sanctuary, received the cutting-edge treatment for a torn anterior cruciate ligament in her right knee, thanks to its Florida developer, Stemlogix LLC in Weston, and the Florida Veterinary League in Vero Beach.

The procedure, which normally would cost about $2,000, uses an animal's own fat to obtain adult stem cells, which are then injected into the problem area to stimulate growth of healthy cells.

Save-the-Chimps Veterinarian Dr. Linda Gregard handled the stem cell recovery procedures. Under anesthesia, fat was removed from chimp's abdomen Wednesday morning and transported to Dr. Darrell Nazareth at the Florida Veterinary League. Nazareth then isolated stem and regenerative cells from the fat, suspended them in platelet-rich plasma and transported the stem cells back to the sanctuary for the chimp's treatment.

"Hopefully, it stops the inflammation and encourages the injury to heal," said Nazareth, who has performed a similar treatment on 15 dogs and cats from his practice. He estimates that within two to three weeks, improvement will be seen in both the chimp's mobility and pain level.

Angie the chimp undergoes revolutionary stem cell treatment

By adminMay 11, 2012Stem Cell Treatment

Chimp receives stem cells 25-year-old chimp received treatments for a torn ...

Photo by Eric Hasert

FORT PIERCE A 25-year-old female chimpanzee at the Save-the-Chimps sanctuary in Fort Pierce may be able to run again, thanks to a revolutionary stem cell treatment performed on Wednesday.

The procedure, which normally would cost about $2,000, uses an animal's own fat to obtain adult stem cells, which are then injected into the problem area to stimulate growth of healthy cells.

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Angie the chimp undergoes revolutionary stem cell treatment