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New gene therapies are a near-cure for Louisiana’s sickle cell patients. But at $1-2M, who will pay for it? – NOLA.com

When Leola Conleys daughter was born, everybody said she looked like she was already three months old.

The girl was 8 pounds, 13 ounces, cute, with a full head of hair, said Conley, a counselor in Lafayette.

About a week after her birth, a letter arrived from the state Department of Health. A standard pinprick on the babys heel at birth showed a mutation in a gene that makes red blood cells. Usually, red blood cells are round, like a doughnut. This babys cells were shaped like a crescent moon. She had sickle cell disease, a genetic disorder that affects about one in every 365 Black babies. Those with severe cases have a life expectancy of about 45 years.

It put me in a depression like nobody's business, said Conley. It broke me, because she looked so healthy.

Now 23, Conleys daughter, Adria, guesses shes been hospitalized about 30 times. She graduated with her MBA in December but is limiting her job search to positions with good insurance close to her doctors in south Louisiana. She doesnt look sick, but unpredictable pain episodes can throw her life off track, requiring weeks of recovery.

But new gene therapies two of which will submit for FDA approval by the end of March offer what could be a life-changing alternative for sickle cell patients in Louisiana,which has the second-highest prevalence of sickle cell disease among Medicaid patients. The problem? At a predicted $1 million to $2 million for the therapy, its unclear how states will pay for it.

If just 7% of the eligible sickle cell population of Louisiana wanted the treatment, it would cost the state a predicted $31.6 million in the first year, according to an analysis published in JAMA Pediatrics by Dr. Patrick DeMartino, a physician specializing in blood diseases among children. Thats for just 17 patients.

Nobody really knows whether it's going to be three people getting the gene therapy in the first year or 15, said DeMartino. And that upper end of 15 to 20 people a year would almost certainly be very consequential for the Medicaid budget.

No one tracks how many Louisiana residents have sickle cell disease, though efforts to create a registry are underway. There are at least 3,000 people on Medicaid with sickle cell disease, according to the state. The 1,400 who were hospitalized in Louisiana in 2020 spent an average of 30 days in the hospital. About one in 10 children with sickle cell anemia will have a symptomatic stroke by age 20, and more have strokes that go undetected.

These people do not live normal lives at all, said DeMartino. The existing therapies are supportive. They don't change the underlying disease, and people still have massive amounts of disability.

Instead of easily transporting oxygen through their bodies, the warped cells become wedged in blood vessels, causing blockages and injuring the vessels, resulting in immense pain.

Have you ever felt your heart beating in your ear? It feels like that but all over your body, said Whitney Carter, a 37-year-old who works from home for an insurance company in Baton Rouge.

The sickled cells getting jammed in her blood vessels feels "like a walnut trying to fit through a straw.

Without a potential pain episode looming over Rhonda Chubes 9-year-old son, he would be able to keep up with his older brothers, play football, swim and collect rocks to his hearts content. With treatment, hed be a regular kid, said Chube, a child care assistant at a Baton Rouge hospital.

His little spirit, he wants to do more, keep going, said Chube. But his little body won't let him.

Prior to gene therapy, a bone-marrow transplant was the only thing close to a cure, but required a close genetic match, usually a sibling, resulting in a match for about 25% of patients. There is also risk that the body will reject the transplant, causing serious complications or death.

Researchers shy away from using the word cure to describe gene therapy, because its not yet clear how long its effects last since the first was given in 2017. But it has been shown to deliver a pain-free existence for trial participants for the first time in their lives.

There are two different gene therapy drugs to treat sickle cell expected to be approved soon one from Bluebird Bio and the other from Vertex and CRISPR Therapeutics.

Its highly transformative, said Dr. Julie Kanter, a hematologist at the University of Alabama at Birmingham who oversees a Bluebird trial that includes patients from Louisiana.

Whats most notable when I look at my patients is how many are now working that couldnt work before, said Kanter. I have one in nursing school, one in a full-time construction job They dont have to worry about being too far from a hospital or too far from pain medicines.

But how to deliver the medications, which will require an expensive and lengthy hospital stay not included in the drugs price tag, is something the world is trying to figure out right now, said Kanter, a New Orleans native who was previously head of the Sickle Cell Center of Southern Louisiana.

Figuring out a way to fund sickle cell treatment is a priority of the Biden administration. And Louisiana has gotten creative with paying for expensive therapies before, Dr. Joe Kanter, state health officer, pointed out. The state got unlimited access to pricey hepatitis C medication for five years for a lump sum instead of paying per dose.

I think the hepatitis C work laid a good groundwork for the feds and other states to follow if they choose to, said Kanter, who is not related to Julie Kanter.

The U.S. Department of Health and Human Services released a plan in mid-February that aims to address the sky-high cost of gene therapy. A pilot program would allow the federal government to coordinate a way to pay on behalf of states. For example, Medicaid might negotiate to pay for treatment in installments dependent on the therapy continuing to prevent pain, which has not yet been proven.

The plan will likely take years to implement.

Still, not everyone will want the therapy, which requires a long hospital stay and chemotherapy to make room for new stem cells.

For many patients, even words like transformative and life-changing can seem empty after years of mistreatment.

Every time I go to the hospital, I feel like I have to put my shield on, because Im going to battle, said Leola Conley. Who will I fight today?

Conley and her daughter have endured 11-hour waits in emergency rooms to get admitted and health care workers who dont believe her pain.

A lack of investment in treatment for sickle cell patients means that the only option for some is often opioids in an emergency room, said Dr. Jennifer Avegno, an emergency medicine physician and director of the New Orleans Health Department.

"Being on chronic opiates is really a terrible way to live," said Avegno. "We've really taken a terrible disease and made it worse in America."

In comparison to sickle cell, genetic diseases that mainly affect White people, such as cystic fibrosis, have much more investment, Avegno pointed out. A federal study found funding for cystic fibrosis was 11 times that of per-person funding for sickle cell disease, even though it impacts about one-third of the number of people.

In a world without sickle cell,Adria Conleyimagines shed move to the Netherlands, where shes read people are happy.Maybe one day, with gene therapy, that will be a reality. For now, sickle cell is calling the shots.

If gene therapy was offered as a safe and affordable option, we would run to it, said Leola Conley. Her fear is that one day, her daughter will be in pain and she wont be around to make sure she gets the right treatment.

Before I leave this earth, I hope to heck my child can be cured, said Conley. She has so much to offer the world.

More here:
New gene therapies are a near-cure for Louisiana's sickle cell patients. But at $1-2M, who will pay for it? - NOLA.com

Medical tourism to Mexico is on the rise, but it can come with risks – WMUR Manchester

One of the four Americans who were kidnapped in Mexico last week was traveling for medical tourism, a friend said. A growing number of U.S. residents are traveling internationally to seek more affordable medical care, more timely care or access to certain treatments or procedures that are unapproved or unavailable in the United States.Latavia "Tay" Washington McGee, 33, drove to Mexico with Shaeed Woodard, Zindell Brown and Eric Williams for cosmetic surgery that was scheduled to take place Friday, according to a close friend of Washington McGee's who did not want to be identified.The four Americans were found Tuesday near the border city of Matamoros, officials said. Washington McGee and Williams were found alive, and Woodard and Brown were found dead, a U.S. official familiar with the investigation told CNN. Investigators are still piecing together what happened after they were abducted.Video below: Hear from the family of some of the Americans recently kidnapped in Mexico.Medical tourism takes people all over the world, including to Mexico, India and Eastern Europe. Violence against medical tourists is generally thought to be rare, but the U.S. Centers for Disease Control and Prevention warns about other risks such as quality of care, infection control and communication challenges with medical staff."It's on the daily, without a doubt. There are people going daily to get this kind of stuff done," said Dr. Nolan Perez, a gastroenterologist in Brownsville, Texas, which is across the border from Matamoros. "Whether it's primary care provider visits or dental procedures or something more significant, like elective or weight loss surgery, there's no doubt that people are doing that because of low cost and easier access."A growing trend One study published in the American Journal of Medicine estimated that fewer than 800,000 Americans traveled to other countries for medical care in 2007, but by 2017, more than 1 million did. More current estimates suggest that those numbers have continued to grow.Video below: Americans are saving by traveling abroad for medical care"People travel because there may be a long waiting time, wait lists or other reasons why they can't get treatment as quickly as they would like it. So they explore their options outside the United States to see what's available," said Elizabeth Ziemba, president of Medical Tourism Training, which provides training and accreditation to international health travel organizations.Also, "price is a big issue in the United States. We know that the US health care system is incredibly expensive," she added. "Even for people with insurance, there may be high deductibles or out-of-pocket costs that are not covered by insurance, so that people will look based on price for what's available in other destinations."The most common procedures that prompt medical tourism trips include dental care, surgery, cosmetic surgery, fertility treatments, organ and tissue transplants and cancer treatment, according to the CDC."With Mexico and Costa Rica, it's overwhelmingly dental and cosmetic surgery. However, certain countries are known for specialties. For example, in Singapore, stem cell and oncology is huge. In India, South India and Chennai Apollo hospitals does incredible work with hip and knee surgeries," Josef Woodman, founder of Patients Beyond Borders, an international health care consulting company, said."In Eastern Europe, a lot of people from the UK but also people from the United States travel to Hungary, Croatia and Turkey for everything from dental to light cosmetic surgery," he said.Mexico is the second most popular destination for medical tourism globally, with an estimated 1.4 million to 3 million people coming into the country to take advantage of inexpensive treatment in 2020, according to Patients Beyond Borders. Matamoros where officials said the four kidnapped Americans were found is "not considered a primary medical travel destination," Woodman said, "largely because there are no internationally accredited medical centers/specialty clinics there or in the immediate region."Mexico City, Cancun and Tijuana are more frequented and reliable destinations in the country, Woodman said.On average, Americans can save 40% to 60% across the most common major procedures received by medical tourists in Mexico, according to an analysis of 2020 health ministry data conducted by Patients Beyond Borders.Woodman said that violence against medical tourists was extremely rare, but he added that "price shopping" searching for the cheapest location for a procedure is a "blueprint for trouble," namely substandard medical care.Video below: U.S. Attorney General Merrick Garland offers remarks about the Americans who were kidnapped in Mexico.'There are the complexities' Medical tourism can be dangerous, depending on the destination and the person's condition."There are the complexities of traveling if you have a medically complex situation. There are fit-to-fly rules. And your health care providers should take into consideration the impact of traveling if you have orthopedic injuries or issues," Ziemba said."The quality of care may be an unknown," she said. "It may be that the quality of care is not up to the standards that you would like. So there's a bit of an unknown there, and then the last thing I would say is, if something goes wrong, what's going to happen?"Perez said he commonly manages complications from medical tourism in his practice."There are a lot of bad outcomes. There are a lot of infections and a lot of botched procedures gone wrong, and patients have to come back to the United States and then have a revision of the surgery," he said. "So it's really unfortunate."Yet Ziemba added that there can be benefits to medical tourism, including that someone could receive a service that they need faster overseas than locally."And price: If you simply can't afford the out-of-pocket costs of health care in the United States, and assuming the risks involved, it may make much more sense for you financially to travel outside the United States," she said.Medical tourism is not just for people traveling around the world. Many living along the U.S.-Mexico border, where access to health care can be scarce, cross into Mexico for care.The Rio Grande Valley, at the southernmost point of Texas, is considered to be a medically underserved area. The region has some of the nation's highest rates of comorbidities, including obesity and diabetes, and one of the lowest physician-to-patient ratios.There is a "dire need" for health care professionals along the border, Perez said."There are not as many doctors given our big and our growing population down here. So the demands on primary care doctors and specialists are very high because there are not enough of us for this population," he said. "So that's one reason why people end up going to Mexico to visit with physicians, because of easier access."People interested in medical tourism can take some steps to help minimize their risk, the CDC says.Those planning to travel to another country for medical care should see their health care provider or a travel medicine provider at least four to six weeks before the trip and get international travel health insurance that covers medical evacuation back to the United States.The CDC advises taking copies of your medical records with you and checking the qualifications of the providers who will be overseeing your medical care. Also, make sure you can get any follow-up care you may need.

One of the four Americans who were kidnapped in Mexico last week was traveling for medical tourism, a friend said.

A growing number of U.S. residents are traveling internationally to seek more affordable medical care, more timely care or access to certain treatments or procedures that are unapproved or unavailable in the United States.

Latavia "Tay" Washington McGee, 33, drove to Mexico with Shaeed Woodard, Zindell Brown and Eric Williams for cosmetic surgery that was scheduled to take place Friday, according to a close friend of Washington McGee's who did not want to be identified.

The four Americans were found Tuesday near the border city of Matamoros, officials said. Washington McGee and Williams were found alive, and Woodard and Brown were found dead, a U.S. official familiar with the investigation told CNN.

Investigators are still piecing together what happened after they were abducted.

Video below: Hear from the family of some of the Americans recently kidnapped in Mexico.

Medical tourism takes people all over the world, including to Mexico, India and Eastern Europe. Violence against medical tourists is generally thought to be rare, but the U.S. Centers for Disease Control and Prevention warns about other risks such as quality of care, infection control and communication challenges with medical staff.

"It's on the daily, without a doubt. There are people going daily to get this kind of stuff done," said Dr. Nolan Perez, a gastroenterologist in Brownsville, Texas, which is across the border from Matamoros. "Whether it's primary care provider visits or dental procedures or something more significant, like elective or weight loss surgery, there's no doubt that people are doing that because of low cost and easier access."

One study published in the American Journal of Medicine estimated that fewer than 800,000 Americans traveled to other countries for medical care in 2007, but by 2017, more than 1 million did. More current estimates suggest that those numbers have continued to grow.

Video below: Americans are saving by traveling abroad for medical care

"People travel because there may be a long waiting time, wait lists or other reasons why they can't get treatment as quickly as they would like it. So they explore their options outside the United States to see what's available," said Elizabeth Ziemba, president of Medical Tourism Training, which provides training and accreditation to international health travel organizations.

Also, "price is a big issue in the United States. We know that the US health care system is incredibly expensive," she added. "Even for people with insurance, there may be high deductibles or out-of-pocket costs that are not covered by insurance, so that people will look based on price for what's available in other destinations."

The most common procedures that prompt medical tourism trips include dental care, surgery, cosmetic surgery, fertility treatments, organ and tissue transplants and cancer treatment, according to the CDC.

"With Mexico and Costa Rica, it's overwhelmingly dental and cosmetic surgery. However, certain countries are known for specialties. For example, in Singapore, stem cell and oncology is huge. In India, South India and Chennai Apollo hospitals does incredible work with hip and knee surgeries," Josef Woodman, founder of Patients Beyond Borders, an international health care consulting company, said.

"In Eastern Europe, a lot of people from the UK but also people from the United States travel to Hungary, Croatia and Turkey for everything from dental to light cosmetic surgery," he said.

Mexico is the second most popular destination for medical tourism globally, with an estimated 1.4 million to 3 million people coming into the country to take advantage of inexpensive treatment in 2020, according to Patients Beyond Borders.

Matamoros where officials said the four kidnapped Americans were found is "not considered a primary medical travel destination," Woodman said, "largely because there are no internationally accredited medical centers/specialty clinics there or in the immediate region."

Mexico City, Cancun and Tijuana are more frequented and reliable destinations in the country, Woodman said.

On average, Americans can save 40% to 60% across the most common major procedures received by medical tourists in Mexico, according to an analysis of 2020 health ministry data conducted by Patients Beyond Borders.

Woodman said that violence against medical tourists was extremely rare, but he added that "price shopping" searching for the cheapest location for a procedure is a "blueprint for trouble," namely substandard medical care.

Video below: U.S. Attorney General Merrick Garland offers remarks about the Americans who were kidnapped in Mexico.

Medical tourism can be dangerous, depending on the destination and the person's condition.

"There are the complexities of traveling if you have a medically complex situation. There are fit-to-fly rules. And your health care providers should take into consideration the impact of traveling if you have orthopedic injuries or issues," Ziemba said.

"The quality of care may be an unknown," she said. "It may be that the quality of care is not up to the standards that you would like. So there's a bit of an unknown there, and then the last thing I would say is, if something goes wrong, what's going to happen?"

Perez said he commonly manages complications from medical tourism in his practice.

"There are a lot of bad outcomes. There are a lot of infections and a lot of botched procedures gone wrong, and patients have to come back to the United States and then have a revision of the surgery," he said. "So it's really unfortunate."

Yet Ziemba added that there can be benefits to medical tourism, including that someone could receive a service that they need faster overseas than locally.

"And price: If you simply can't afford the out-of-pocket costs of health care in the United States, and assuming the risks involved, it may make much more sense for you financially to travel outside the United States," she said.

Medical tourism is not just for people traveling around the world. Many living along the U.S.-Mexico border, where access to health care can be scarce, cross into Mexico for care.

The Rio Grande Valley, at the southernmost point of Texas, is considered to be a medically underserved area. The region has some of the nation's highest rates of comorbidities, including obesity and diabetes, and one of the lowest physician-to-patient ratios.

There is a "dire need" for health care professionals along the border, Perez said.

"There are not as many doctors given our big and our growing population down here. So the demands on primary care doctors and specialists are very high because there are not enough of us for this population," he said. "So that's one reason why people end up going to Mexico to visit with physicians, because of easier access."

People interested in medical tourism can take some steps to help minimize their risk, the CDC says.

Those planning to travel to another country for medical care should see their health care provider or a travel medicine provider at least four to six weeks before the trip and get international travel health insurance that covers medical evacuation back to the United States.

The CDC advises taking copies of your medical records with you and checking the qualifications of the providers who will be overseeing your medical care. Also, make sure you can get any follow-up care you may need.

Excerpt from:
Medical tourism to Mexico is on the rise, but it can come with risks - WMUR Manchester

Michael York: ‘I have not lost anything. In fact, I think I’ve gained’ – Rochester Post Bulletin

Michael York has been called the quintessential British actor. Dashing, debonair, and intelligent. One of the most sought-after movie stars of his time.

And thats just in the first paragraph of one magazine article.

He grew up as the second of four children of a musician mother and an ex-Royal Artillery British Army officer/businessman father in Oxfordshire, the southeast county in England that connects the Cotswolds to the Chilterns (rolling hills to more rolling hills). Was schooled at Bromley Grammar School for Boys then Oxford. Joined the National Youth Theatre as a teen.

Landed a role in a BBC-TV movie. Made the jump to the big screen as Tybalt in the 1968 film version of Romeo and Juliet. Found fame as Brian Roberts in 1972s Cabaret and as DArtagnan in The Three Musketeers a year later.

Played John the Baptist in Jesus of Nazareth. The Antichrist in The Omega Code. Logan in Logans Run.

Narrated the entire New King James Version for an audio Bible. Voiced Dr. Lionel Budgie on The Simpsons. Wrote five well-received books (including The Readiness Is All: My Odyssey of Healing from Mayo Clinic to John of God and Beyond.)

Played Basil Exposition in Austin Powers. Professor Asher Fleming on Gilmore Girls. Himself on Curb Your Enthusiasm.

Received the OBE (The Most Excellent Order of the British Empire). Won two Emmys. Was voted to the International Best Dressed List Hall of Fame.

Lunched with Princess Grace in Monaco. Outbidand this is the kind of name-dropping sentence you dont often get to sayCary Grant for a David Hockney painting. Called Lawrence Olivier Larry.

Contributed

Today, Michael York, 80, walks through the lobby of Charter Housethe 350-resident downtown retirement community with a focus on healthy agingand wishes Happy Birthday to longtime Rochesterite Tom Ostrom, says Good morning, Theresa as he picks up a copy of the daily Charter House newsletter, The Chronicle (with inspirational quotes and celeb birthdays), and greets me like we know each other.

We grab coffees in the Corner Cupboard (the Charter House cafe/gift shop, Hello, Carol!) and elevator up to his apartment, where wife Patriciashe often goes by Patwarmly welcomes us into their fourth-floor apartment.

Steve Lange [showing Michael the January issue of Rochester Magazine]: This is the January issue, which includes The Rochies, some of our best and worst stories of the past year. In the best category was Michael York moves to Rochester.

Michael York: Well, Im flattered. Im truly flattered.

Steve: Youve been nominated for two Emmys, won a Satellite Award and two Lifetime Achievement awards. Where does this stack up in those awards?

Michael: Why, this is right at the top.

Steve: You are off to a good start. Was this a typical morning for you, except for me coming here?

Michael: Well, we got coffee downstairs. We can easily have a coffee in the kitchen, but I like to get out and see whats going on and pick up a copy of The Chronicle, the newsletter that they produce here. Its a wonderful mixture of the serious and the ridiculous. I love it. Im totally hooked on it. And then we will often walk around. I think its good to interact with people rather than to be solitary. It feels like being back at university here. Theres everythingintellectual, spiritual, and physical.

Contributed

Steve: Since you mentioned university, Im going to give you some names: C.J. Lukey, R.V. Neve, P.S. Fenwick, D.E. Tennant, K.V. Turpee ...

Michael: Thats the 61 class from Bromley Grammar School [in London].

Steve: [showing a photo of the class].

Michael: My name was Johnson, then. M.H. Johnson. I think I was school captain. How did you get this? I dont believe Ive ever seen this.

Steve: I do my research. Charter House takes you back to those Bromley days, those Oxford days?

Michael: It does. Absolutely. And I love being on this sort of campus because I like to walk to places. It also reminds me of university because there are so many brilliant people together on this one campus.

Steve: I reached out to a few doctor friends of mine about your doctor, Robert Kyle. They described him as a world-renowned expert in hematology.

Michael: He is amazing. Pat, explain how Dr. Kyle came into our life.

Contributed / Pat York

Pat York has been called a major voice in the world of photography.

She was born Patricia Watson in Jamaica to an English diplomat father and an English mother, attended a French convent school in England and was tutored in Germany, eloped as a teenager (for a short-lived marriage) and gave birth to a son, Rick (a film producer).

The artist-turned-photographer soon became the portraitist for the stars (Andy Warhol, the Kennedys, John Travolta). When she got tired of shooting celebrities, York turned to artistic photography.

Her 2004 book, Pat York: Covered-Uncovered starts with her celebrity photos. Part two focuses on ordinary peopleincluding their plumberwho agreed to be photographed in the nude, while working. Part three features Pats groundbreaking work photographing human cadavers.

As a couple, Michael and Pat have been described as a binary star, each in orbit around the other.

So, as soon as Michael asks Pat about anything during this interview, she jumps in seamlessly.

Pat: I will first tell you how I met Michael. I lived in New York, and I worked for Vogue and Glamour. My magazine editor sent me to photograph Tom Stoppard [a playwright and screenwriter] in London. I was also shooting the new young hot actor called Michael York, who had just come out in two movies. And we were both engaged to someone. I was engaged to S.I. Newhouse [the billionaire magazine publisher], though I had asked him to wait for us to set a marriage date. Well, Michael and I just clicked. We both broke off our engagements. He proposed on Juhu Beach in Mumbai on Valentines Day of 1967. We were married in 1968.

Steve: As far as contacting Dr. Kyle, I know that was an important decision, since you are big believers in complementary and alternative medicine.

Contributed

Pat: So I was very worried that Michael, in L.A., was getting misdiagnosed. But I didnt know for sure. But I didnt feel confident. He was being treated for multiple myeloma. Then a really nice doctor in L.A. gave me Dr. Kyles number at Mayo, and we became friends on email and phone calls. Dr. Kyle, at one point said to me, Well, what are his symptoms? And I told him about everything, including the purpura [a rash of purple spots] around the eyes. And he said, You know, I dont think hes got multiple myeloma. I think hes got amyloidosis.

Steve: That was like 10 years ago?

Pat: We came here in 2012. Michael had a checkup. They decided it was amyloidosis [a rare disease that occurs when a protein called amyloid builds up in organs]. And it was suggested he have his first stem cell transplant, which was a big success. And our whole life had been nothing but travel. We were in our house maybe two months a year. So we continued that life and we came back here about every five months for a checkup. Then in 2018, he needed a second stem cell transplant. We stayed in a guest apartment here. We decided last year that we should move here definitely.

Steve: Michael, you describe amyloidosis as the Rodney Dangerfield of diseases. Thats a great line.

Michael: Yes. At that time, amyloidosis was not fully on the radar. It was there, but it was little appreciated. But since then, its become extremely well-known and extremely well-provided for. Now, I see a Dr. [Morrie] Gertz every month or six weeks or so. Its been a wonderful place.

Steve: What are your hobbies? I know you and Pat played some late-night Scrabble with Jane Fonda ...

Michael: Well, I must say I like watching movies.

Steve: Whats the last movie you watched?

Michael: Oh, it was wonderful. Im trying to think of the name of it ... Seabiscuit.

Contributed / Pat York.

Steve: Thats a good one. Im going to give you some of your own movie descriptions. You tell me what movie it is. I will read them like an announcer reading for a trailer.

Micheal: I cant wait to hear it.

Steve [in announcer voice]: While escaping war-torn China, a group of Europeans crash in the Himalayas, where they are rescued and taken to the mysterious Valley of the Blue Moon, Shangri-La.

Michael: Lost Horizon.

Steve. Yes. A Russian immigrant finds himself in bed with the mob after buying a sexual novelties shop.

Michael: I remember. I think they changed titles. Did they end up calling it Merchants of Venus?

Steve: Yes. Last one: Sparks flyand the heat is literally turned upwhen Dr. Kornack goes undercover as a pizza oven salesman and seduces notorious deep-dish lover Dame Pomeroy to solve the mystery of Kornacks missing Weimaraner.

Michael: I cant believe this. I know Ive had some gaps in my memory lately, but I would remember this one.

Contributed / Michael York.

Steve: Youre right. I made that one up. OK. Whats the best restaurant meal in Rochester?

Michael: Well, we like the fish restaurant ... Pescara. Also, Terza and Twigs.

Steve: Thats good, because theyre all advertisers.

Michael: We love trying the local restaurants.

Steve: One of the first movies I saw in the theater was Logans Run in 1976. I was seven years old, and it had a real impact. I know youve been heralded as a serious Shakespearean actor, but Logans Run stuck with me.

Michael: I got the script and turned it down. I didnt think it was for me. At that time, I was doing a play in L.A. ... The theater company hired a young man to drive me to the theater, and we became friends. I got the script and I didnt think it was for me. And this kid driving me, he saw the script and said Do you mind if I read this? I said, Oh, of course. I came back. He was wagging a finger. He said, You dont understand whats going on, but I do. You need to do this movie. So I did. He did me a real favor. So always listen to your chauffeur.

Steve: I will remember to do that. Whats your favorite photo from your wifes portfolio?

Michael [pointing to a large framed print above the table]: This one.

Contributed / Tsuni / USA / Alamy

Steve: I was going to guess it was her shot of Jane Fonda in her Barbarella outfit standing over a stove cooking dinner.

Michael: How interesting. Thats not my favorite, but one of the doctors here loved it.

Steve: I was going to guess your favorite was not Plumber, 1997. Thats the photo of your naked plumber working under your sink.

Michael: Oh, no.

Steve: Im going to just throw out the most name-dropping sentences imaginable. You won a David Hockney painting by outbidding Cary Grant.

Michael: Well, we got the Hockney. And then Cary was forever trying to buy it back. Cary became a good friend. I was resident in Monaco, in Monte Carlo, and knew Grace [Kelly] very well. And she would invite us to lunch. And one time, Cary Grant was also there. He came down the steps into the reception area and fell into Pats arms. He said, Theres no place Id rather be.

Steve: Wow. The only comparable story in my life would probably be when I was a celebrity parade judge at the Viola Gopher Count, with Tom Overlie ...

Michael: That sounds lovely.

Steve: Yes. Right. OK. You sold your $7 million house overlooking the Sunset Strip in Hollywood Hills. You had a French chef you brought in for special meals. All of that. What do you miss most about that life now that youre in Rochester?

Michael: I dont miss any of it. I have not lost anything. In fact, I think Ive gained. We get to live in a part of the world we want to get to know. We love exploring the area. Weve toured an amazing art museum in Winona, the Minnesota Marine Art Museum. Weve been on the Mississippi River with a wonderful, fantastic captain who was like a professor. He told us so much. And then he took us to this place with these wonderful eagles and then they were flying above our heads. It was extraordinary. We even went to Austin for the Spam Museum. I sent postcards to all my contemporaries in the university. Ive booked a cruise on the Great Lakes leaving from Toronto this June. We have so much to see here.

Steve: That is great. You dont sound like someone with many regrets.

Michael: I dont believe in them. Regrets dont do anyone any good.

With that, Michael and Pat show me to the door, offer to walk me out. I know my way, though, and I know they have plenty to do.

Return a call from an art studio hoping to display some of Pats paintings. Meet a group for their regular exercise class. Prepare for dinner at the Skyview Dining Room (Charter Houses restaurant).

Plan a daytrip to Lanesboro for Amish Country. Find a good Indian restaurant to celebrate the 55th anniversary on Michaels wedding proposal. And, tomorrow morning, take that walk down to the Corner Cupboard for coffee with Rochester friends.

Read more:
Michael York: 'I have not lost anything. In fact, I think I've gained' - Rochester Post Bulletin

New stem cell model developed for treatment of newborn lung … – Innovation Origins

Congenital diaphragmatic hernia is one of the deadliest birth defects. To better understand and treat this condition in the future, an international team of researchers involving Leipzig University Hospital designed a new cell model in the laboratory and tested a drug therapy on it, the institution announced in a press release. The promising results of their recent study have been published in the prestigious American Journal of Respiratory and Critical Care Medicine.

Congenital diaphragmatic hernia (CDH) is a common condition, affecting one in every 2500births. Up to 30per cent of affected babies die from it. The main problem is the underdeveloped lung. The condition also involves a hole in the diaphragm, which paediatric surgeons correct by closing it in the first week of life. Until now, there has been little medical knowledge about how CDH develops what exactly goes wrong during embryonic development. Dr Richard Wagner, a paediatric surgeon and scientist at Leipzig University Hospital, teamed up with researchers from Massachusetts General Hospital in Boston to establish a new patient-specific cell model at Harvard Medical School. Using their model, the researchers investigated possible ways of treating this condition.

German stem cell research has its eye on retinal diseases

Human-induced pluripotent stem cells (HiPSCs) can be used to generate brain organoids that have an ocular structure.

Babies with CDH are placed on a ventilator immediately after birth. We were able to isolate stem cells from the fluid that is sucked out of the childrens lungs and otherwise disposed of, and grow them in the laboratory, explains Dr Wagner, first author of the study. While working as a postdoctoral researcher in the US, the doctor from Leipzig examined the stem cells in the laboratory together with the American researchers, designing cell models of the airways of the tiny patients. This gave them access for the first time to living human lung tissue from patients with CDH. They then compared the stem cells from healthy and underdeveloped lungs.

When the researchers looked at the molecular properties of the stem cells, they found that they had been altered due to inflammation. However, drug therapy in the cell model was able to restore functionality. We also tested this process in animal models and showed that the treatment contributed to better lung development there, too. With the same drug therapy, we were therefore able to achieve positive effects both in human cells in the Petri dish and in the living organism in the established animal model, explains Dr Wagner.

The treatment was performed with the steroid dexamethasone. This drug is already used in clinical practice to induce lung maturity in the foetus when there is a risk of premature birth during pregnancy. What is most appealing is the fact that we already know that this drug is not harmful in pregnancy. If we were to collect more data in laboratory research, it would be possible to investigate later in clinical trials whether there are advantages to administering the drug during pregnancy in order to slow down the possible inflammation in the organism and help the lungs grow, says DrWagner, adding that the aim in future is to be able to intervene with a drug directly after the diagnosis of a diaphragmatic hernia, which happens at around the 20thweek of pregnancy.

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Scientists create mice with two fathers after making eggs from male cells – The Guardian

Genetics

Creation of mammal with two biological fathers could pave way for new fertility treatments in humans

Scientists have created mice with two biological fathers by generating eggs from male cells, a development that opens up radical new possibilities for reproduction.

The advance could ultimately pave the way for treatments for severe forms of infertility, as well as raising the tantalising prospect of same-sex couples being able to have a biological child together in the future.

This is the first case of making robust mammal oocytes from male cells, said Katsuhiko Hayashi, who led the work at Kyushu University in Japan and is internationally renowned as a pioneer in the field of lab-grown eggs and sperm.

Hayashi, who presented the development at the Third International Summit on Human Genome Editing at the Francis Crick Institute in London on Wednesday, predicts that it will be technically possible to create a viable human egg from a male skin cell within a decade. Others suggested this timeline was optimistic given that scientists are yet to create viable lab-grown human eggs from female cells.

Previously scientists have created mice that technically had two biological fathers through a chain of elaborate steps, including genetic engineering. However, this is the first time viable eggs have been cultivated from male cells and marks a significant advance. Hayashis team is now attempting to replicate this achievement with human cells, although there would be significant hurdles for the use of lab-grown eggs for clinical purposes, including establishing their safety.

Purely in terms of technology, it will be possible [in humans] even in 10 years, he said, adding that he personally would be in favour of the technology being used clinically to allow two men to have a baby if it were shown to be safe.

I dont know whether theyll be available for reproduction, he said. That is not a question just for the scientific programme, but also for [society].

The technique could also be applied to treat severe forms of infertility, including women with Turners syndrome, in whom one copy of the X chromosome is missing or partly missing, and Hayashi said this application was the primary motivation for the research.

Others suggested that it could prove challenging to translate the technique to human cells. Human cells require much longer periods of cultivation to produce a mature egg, which can increase the risk of cells acquiring unwanted genetic changes.

Prof George Daley, the dean of Harvard Medical School, described the work as fascinating, but added that other research had indicated that creating lab-grown gametes from human cells was more challenging than for mouse cells. We still dont understand enough of the unique biology of human gametogenesis to reproduce Hayashis provocative work in mice, he said.

The study, which has been submitted for publication in a leading journal, relied on a sequence of intricate steps to transform a skin cell, carrying the male XY chromosome combination, into an egg, with the female XX version.

Male skin cells were reprogrammed into a stem cell-like state to create so-called induced pluripotent stem (iPS) cells. The Y-chromosome of these cells was then deleted and replaced by an X chromosome borrowed from another cell to produce iPS cells with two identical X chromosomes.

The trick of this, the biggest trick, is the duplication of the X chromosome, said Hayashi. We really tried to establish a system to duplicate the X chromosome.

Finally, the cells were cultivated in an ovary organoid, a culture system designed to replicate the conditions inside a mouse ovary. When the eggs were fertilised with normal sperm, the scientists obtained about 600 embryos, which were implanted into surrogate mice, resulting in the birth of seven mouse pups. The efficiency of about 1% was lower than the efficiency achieved with normal female-derived eggs, where about 5% of embryos went on to produce a live birth.

The baby mice appeared healthy, had a normal lifespan, and went on to have offspring as adults. They look OK, they look to be growing normally, they become fathers, said Hayashi.

He and colleagues are now attempting to replicate the creation of lab-grown eggs using human cells.

Prof Amander Clark, who works on lab-grown gametes at the University of California Los Angeles, said that translating the work into human cells would be a huge leap, because scientists are yet to create lab-grown human eggs from female cells.

Scientists have created the precursors of human eggs, but until now the cells have stopped developing before the point of meiosis, a critical step of cell division that is required in the development of mature eggs and sperm. Were poised at this bottleneck at the moment, she said. The next steps are an engineering challenge. But getting through that could be 10 years or 20 years.

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Researchers discover therapeutic target to aid in glaucoma treatment – Science Daily

Indiana University School of Medicine researchers have identified a new therapeutic target that could lead to more effective treatment of glaucoma.

Glaucoma is a neurodegenerative disease that causes vision loss and blindness due to a damaged optic nerve. More than 200,000 people are affected by glaucoma in the United States each year. Unfortunately, there is currently no treatment. In a newly published paper in Communications Biology, researchers found neurons use mitochondria for a steady source of energy, and restoring mitochondrial homeostasis in the diseased neurons can protect the optic nerve cells from being damaged.

"Age-related neurodegenerative disease, which includes glaucoma, Parkinson's disease, and amyotrophic lateral sclerosis (ALS), is the biggest global health problem," said Arupratan Das, PhD, assistant professor of ophthalmology and principal investigator of the study. "The fundamental mechanisms that we discovered can be used to protect neurons in glaucoma and be tested for the other diseases. We have identified a critical step of complex mitochondrial homeostasis process, which rejuvenates the dying neuron, similar to giving a lifeline to a dying person."

The research team, led by Michelle Surma and Kavitha Anbarasu from the Department of Ophthalmology, used induced pluripotent stem cells (iPSCs) from patients with and without glaucoma as well as clustered regularly interspaced short palindromic repeats (CRISPR) engineered human embryonic stem cells with glaucoma mutation. Using stem cell differentiated retinal ganglion cells (hRGCs) of the optic nerve, electron microscopy and metabolic analysis, researchers identified glaucomatous retinal ganglion cells suffer mitochondrial deficiency with more metabolic burden on each mitochondrion. This leads to mitochondrial damage and degeneration. Mitochondria are the tube like structures in cells which produce adenosine triphosphate, cell's energy source.

However, the process could be reversed by enhancing mitochondrial biogenesis by a pharmacological agent. The team showed retinal ganglion cells are highly efficient in degrading bad mitochondria, but at the same time producing more to maintain homeostasis.

"Finding that retinal ganglion cells with glaucoma produce more adenosine triphosphate even with less mitochondria was astonishing," Das said. "However, when triggered to produce more mitochondria, the adenosine triphosphate production load was distributed among more mitochondrion which restored the organelle physiology. It is similar to a situation where a heavy stone is carried by fewer people versus a greater number of people -- each person will have less pain and injury, just like each mitochondrion will have less difficulty and damage."

In the future, Das would like to test if these mechanisms protect the optic nerve in animal models under injury before testing in humans to hopefully lead to new clinical interventions.

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Identifying potential treatments for ALS – National Institutes of Health (.gov)

March 7, 2023

Amyotrophic lateral sclerosis, or ALS, is a rare but devastating neurological disease. In ALS, misfolded proteins build up within motor neuronsthe nerve cells in the brain and spinal cord that control voluntary muscle movement. The inability to clear this toxic protein buildup leads to muscle weakness, paralysis, and eventually death.

Some cases of ALS are caused by known, inherited genetic mutations. But most are from sporadic, unknown causes. Rather than target each genetic cause of ALS, researchers have been seeking treatments that could be used across different types. An NIH-funded research team led by Dr. Justin Ichida from the University of Southern California has been searching for cellular processes that couldbe manipulated to treat ALS regardless of the genetic drivers of a persons disease.

The first of two new studies from the team was published in Cell on February 16, 2023. In earlier work, they found that compounds that blocked a protein called PIKFYVE kinase extended the lives of ALS motor neurons. One of these compounds was a small molecule called apilimod.

In their follow-up study, the team tested apilimod in motor neurons with many different drivers of ALS. They also used several genetic methods to shut down PIKFYVE. All methods of PIKFYVE inhibition extended the lives of the various ALS neuron types tested.

Further work teased out the cellular mechanisms responsible for this protective effect. The researchers found that inhibiting PIKFYVE helped neurons clear misfolded, toxic proteins. This happened because a waste-removal process called exocytosis became activated when PIKFYVE was shut down.

The toxic version of a protein called TDP-43 has been linked to ALS and other neurodegenerative diseases. This protein was effectively cleared from the cells through exocytosis after PIKFYVE was inhibited.

When the researchers blocked PIKFYVE in several animal models of ALSincluding mice with a misfolded version of TDP-43motor function was improved, and the animals lived longer.

The teams second study was published on February 2, 2023, in Cell Stem Cell. In that work, the researchers screened a library of almost 2,000 approved drugs and other compounds for their ability to extend the life of ALS motor neurons. They found that some of the most promising compounds altered cellular signaling driven by hormones called androgens (such as testosterone) in the body.

The long-term manipulation of such hormones may have unwanted side effects. So, the researchers searched for other targets that altered gene activity levels in similar ways in motor neurons. Their top candidate was called SFY2.

The team found that suppressing SYF2 levels using genetic techniques increased survival in most types of ALS motor neurons tested, including those that accumulate toxic TDP-43.

In mice, suppressing SYF2 changed the way TDP-43 was exported from the nuclei of cells. This stopped the buildup of toxic protein clumps within neurons. Reducing the amount of SYF2 in the mice improved motor functioning as well.

Our discoveries bring us closer to achieving our big picture goal: finding treatments that can be broadly effective for all patients who suffer from ALS, Ichida says.

Before these approaches could be tested in people, future work will be needed to identify the safest ways to suppress such cellular pathways.

by Sharon Reynolds

References:PIKFYVE inhibition mitigates disease in models of diverse forms of ALS. Hung ST, Linares GR, Chang WH, Eoh Y, Krishnan G, Mendonca S, Hong S, Shi Y, Santana M, Kueth C, Macklin-Isquierdo S, Perry S, Duhaime S, Maios C, Chang J, Perez J, Couto A, Lai J, Li Y, Alworth SV, Hendricks E, Wang Y, Zlokovic BV, Dickman DK, Parker JA, Zarnescu DC, Gao FB,Ichida JK. Cell. 2023 Feb 16;186(4):786-802.e28. doi: 10.1016/j.cell.2023.01.005. Epub 2023 Feb 7. PMID:36754049.

SYF2 suppression mitigates neurodegeneration in models of diverse forms of ALS. Linares GR, Li Y, Chang WH, Rubin-Sigler J, Mendonca S, Hong S, Eoh Y, Guo W, Huang YH, Chang J, Tu S, Dorjsuren N, Santana M, Hung ST, Yu J, Perez J, Chickering M, Cheng TY, Huang CC, Lee SJ, Deng HJ, Bach KT, Gray K, Subramanyam V, Rosenfeld J, Alworth SV, Goodarzi H,Ichida JK. Cell Stem Cell. 2023 Feb 2;30(2):171-187.e14. doi: 10.1016/j.stem.2023.01.005.PMID:36736291.

Funding:NIHs National Institute of Neurological Disorders and Stroke (NINDS); US Department of Defense; Donald E. and Delia B. Baxter Foundation; Tau Consortium; Ford Foundation; Muscular Dystrophy Association; New York Stem Cell Foundation; Alzheimer's Drug Discovery Foundation; Association for Frontotemporal Degeneration; Pape Adams Foundation; John Douglas French Alzheimer's Foundation; Harrington Discovery Institute; Milken Family Foundation; USC Broad Innovation Award; Southern California Clinical and Translational Science Institute; Keck Medicine of USC; Target ALS Foundation; John Douglas French Alzheimers Foundation; Broad Institute; ALS Association; Lawrence and Isabel Barnett Drug Development Award; Frick Foundation for ALS Research; University of Southern California Alzheimers Disease Research Center; California Institute for Regenerative Medicine.

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Identifying potential treatments for ALS - National Institutes of Health (.gov)

Exciting Cell & Gene Therapy Research Updates in Glaucoma and … – geneonline

Exciting Cell & Gene Therapy Research Updates in Glaucoma and Nerve Regeneration

As the Cell & Gene Therapy Research & Development Congress 2023 entered its second day, experts from around the world continued to share their latest exciting research findings and possible future directions in the related fields.

Day two of the event began with a virtual keynote address themed Gene Therapy for Glaucoma by Paul Kaufman, MD, Professor of Ophthalmology & Visual Sciences at the University of Wisconsin-Madison School of Medicine & Public Health. As an incurable, age-related degenerative optic neuropathy, glaucoma affects approximately 80 million people globally and is one of the worlds leading causes of preventable blindness. In most cases, the disease is caused by a buildup of fluid in the front part of the eye, which elevates intraocular pressure (IOP), causing damage to the optic nerve and possibly leading to permanent vision loss. With this in mind, lowering IOP is the top priority in devising ways to treat glaucoma and prevent disease progression.

Following an overview of glaucoma, professor Kaufman introduced some new approaches to managing the disease, with an emphasis on modifying the aqueous humor dynamics in the eye, such as encapsulated cell technology (ECT) and devices for micro-invasive glaucoma surgery (MIGS). Then he focused on the development of gene therapy for glaucoma and the findings obtained by his research team.

Related Article: Cell & Gene Therapy Congress Asia Day One: Advances in the Research

Theoretically, it is possible for gene therapies to achieve IOP reduction and preserve vision by different means such as protecting neurons of the optic nerve, decreasing aqueous humor production and enhancing aqueous humor outflow.

With an interest in the mechanisms of aqueous humor formation and drainage, Prof. Kaufmans team sets their strategy to go after the physiology of the system, focusing on the trabecular meshwork (TM) and the uveoscleral pathway for outflow enhancement. They aim at identifying genes that would act on cells involved in these pathways so as to increase the fluid flow in the eye without actually replacing a gene that happens to be defective. Ultimately, their goal is to deliver a gene into the tissues and obtain a long-lasting therapeutic effect, allowing patients to avoid using time-consuming daily treatments

The team hypothesizes that in live non-human primates (NHPs), administration of viral vector-transgene constructs incorporating the C3 transferase or caldesmon transgene directly into Schlemms canal will decrease conventional aqueous humor outflow resistance and thereby reduce intraocular pressure.

Professor Kaufman also shared the research findings regarding the Wnt signaling pathway in human TM cells, showing that restoring the overexpression of certain proteins in this pathway may be a possible way for IOP reduction, thereby treating glaucoma.

Towards the end of his presentation, Professor Kaufman also mentioned current constraints and challenges of gene therapy for glaucoma, including vector-associated side effects (toxicity), difficulties in turning on or off the gene, gene-targeting technology, regulatory hurdles as well as the gap between theories and experimental observations that have yet to be resolved. Nevertheless, he still feels hopeful that the current research findings may provide insights for developing potential human therapeutics and that they could eventually move into the pre-drug phase and become part of a clinical trial.

The second keynote speech of the day was delivered by professor Ing-Ming Chiu, Chair Professor at Graduate Institute of Biomedical Sciences at China Medical University, with the theme Neural Stem Cells and Neurotrophic Factors in Nerve Regeneration.

Since the discovery of the regeneration of neurons after birth in animals, scientists around the world have been engaging in neural stem cell (NSC) research. It has been found that fibroblast growth factors FGF1 and FGF2 are required for the maintenance of NSCs in early development.

Moreover, FGF1 and interleukin 12 (IL12), separately, are beneficial in nerve repair via promoting axonal growth. With the sciatic nerve injury mouse model, Prof. Chius team has found that the implantation of NSCs combined with nerve conduit and IL12 can increase neuroregeneration and improve motor recovery.

IL12 is a dimeric protein that is formed by the combination of p35 and p40 subunits. In particular, the team has found that mouse IL12p80 (homodimer of two p40 subunits) facilitates nerve regeneration and promotes functional recovery in vivo. In vitro studies of the molecular mechanism reveal that IL12p80 stimulates the Schwann cell differentiation of mouse NSCs through the phosphorylation of Stat3 in NSCs.

Furthermore, the team later used human IL12p80 in mouse models and it has been found that hIL12p80 also facilitates nerve regeneration in mice. One of their ongoing studies involves an attempt of using hIL12 and human nerve in the mouse model. In the event that positive outcomes are achieved, these findings may provide insights for developing potential therapies for neurological diseases in humans.

Developments in Regenerative Medicine: Gene Delivery Tools and Cell Therapy for Parkinsons Disease

2023-03-08

Revolutionizing Eye Disease Treatment with iPSC Therapy

2023-03-07

Global Head of Research of Kite Pharma Unveiled the Key Elements to Success in Adoptive Cell Therapy

2023-03-07

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Exciting Cell & Gene Therapy Research Updates in Glaucoma and ... - geneonline

Gates Grubstake Fund Awards Over $1.5 Million to Campus Researchers – University of Colorado Anschutz Medical Campus

The Gates Grubstake Fund invokes the memory of Gold Rush prospectors who received seed money, grubstakes, for food and supplies so they could search for treasure. The funding supports the work of modern-day prospectors translational researchers affiliated with Gates Institute whose work developing cell- and gene-based therapies could make a difference in human lives. In 2022, four awardees received $350,000 each to support their work.

In addition, second-tranche awards are made to eligible previous Grubstake recipients who have demonstrated success in developing technology toward a clinical trial using original Grubstake funding, along with evidence that additional funding would allow them to reach an additional inflection point toward commercialization.

In total, over $1.5 million was awarded by the Gates Grubstake Fund to CU Anschutz researchers.

Ganna Bilousova, PhD: Somatic Cell Rejuvenation for Skin Transplantation and Wound Healing

With a steady increase in the aging population, the care of acute and chronic wounds in the elderly has become a priority topic for clinicians. Many options to treat wounds are currently available. However, none of them restores the functionality of aged skin cells, hence low success rate in the elderly. Ganna Bilousova, PhD, associate professor of dermatology, and Igor Kogut, PhD, assistant professor of dermatology, are developing a permanent corrective therapy for acute and chronic wounds in the elderly by rejuvenating the patients own skin cells using a patent-pending RNA cocktail of factors. The Grubstake Award will allow their teams to finalize their therapeutic product and compare its efficacy with the competition to accelerate development toward pre-investigational new drug (IND) filing with the Food & Drug Administration.

Mi-Hyun Nam, PhD: Restoration of Vision in Glaucoma Through Cell Therapy

Principal investigator Mi-Hyun Nam, PhD, research instructor in ophthalmology, and her co-PI, Natalia Vergara, PhD, assistant professor of ophthalmology, are developing a human stem cell-based therapy for treating glaucoma, the second-leading cause of blindness worldwide. Current medical therapies are limited to lowering intraocular pressure, which may prevent further vision loss, but no treatment to date can restore vision once it has been lost. The Grubstake funding will enable them to perform preclinical studies to determine the feasibility and efficacy of their therapy.

Eric Kohler, MD, PhD: Adjunctive LAT-Activating Chimeric Antigen Receptor T cells (ALA-CART) Strengths

CAR-T cells have revolutionized the treatment of leukemia and lymphoma, inducing responses against cancers that no longer respond to traditional therapies. However, current CAR-T cell strategies are unable to induce long-term remissions in the majority of patients, owing to limitations in their persistence, potency and sensitivity. By studying CAR-T cell signaling, the lab of Eric Kohler, MD, PhD, assistant professor in the Department of Pediatrics, identified that inefficient activation of the molecule LAT was responsible for many of the limitations in current CAR-T cell therapies. Using this finding, they rationally designed a new Adjunctive LAT-Activating CAR-T cell (ALA-CART) that restores LAT signaling and demonstrates enhanced potency and persistence in preclinical models. Furthermore, ALA-CART cells demonstrated increased sensitivity to tumor cells with low levels of the targeted antigen, allowing for eradication of leukemia that would otherwise not be seen by current CAR-T cell therapies. These advancements hold the potential to close many of the vulnerabilities of CAR-T cell therapies and improve their long-term effectiveness for patients. Grubstake funding will be used to generate safety data and establish manufacturing workflows at the Gates Biomanufacturing Facility to transition this work to clinical trials.

Daniel Sherbenou, MD, PhD: Response Prediction for T Cell Engaging Bispecific Antibodies in Multiple Myeloma

Daniel Sherbenou, MD, PhD, associate professor in hematology, Department of Medicine, received a Gates Grubstake Award to commercialize the new myeloma drug sensitivity testing (My-DST) assay for profiling responses to T cell engaging bispecific antibodies for patients with multiple myeloma, an incurable blood cancer afflicting more than 150,000 Americans. Bispecific antibodies are a promising new class of therapy that redirect a patients own T cells to kill the cancerous myeloma cells. To improve the clinical application of these drugs, My-DST has potential as a new laboratory test for measuring responses of an individual patients tumor cells from biopsy specimens. In this project, Sherbenous team will pursue regulatory approvals and scale up efforts to establish My-DST as a personalize medicine approach for the various bispecific antibodies in clinical use or in clinical trials.

Michael Verneris, MD: Multiomic Approach to Establish Mechanisms of Efficacy of Stem Cell-Derived Innate Lymphoid Cells in Gastrointestinal Tract Repair

Michael Verneris, MD, professor in the Department of Pediatrics, received a second-tranche award of $100,000. Innate lymphoid cells are tissue resident lymphocytes that can be restorative to injured mucosal tissues. In Crohns disease (CD), a subpopulation of ILCs (ILC3s) are depleted and the loss of these cells is thought to be part of the pathogenesis of CD. Replacement of ILC3s in CD may be therapeutic. Verneris laboratory has developed methods to generate ILC3s from hematopoietic stem cells and has found that adoptive transfer of these cells can improve the intestinal dysfunction in TNFdARE mice, which is a model of CD. With this funding they will perform CITE-SEQ and Xenium in situ analysis on ILC3 treated (and untreated) mice to better understand the impact of ILC3s adoptive transfer at single cell resolution. Additionally, they will continue to perform scale-up experiments with the goal of transferring this technology to the Gates Biomanufacturing Facility.

Eduardo Davila, PhD: Manufacturing of Genetically Engineered Tumor Infiltrating Lymphocyte (TIL) Therapy

Eduardo Davila, PhD, professor in the Division of Medical Oncology, was awarded $50,000 in second-tranche funds. This funding will accelerate development toward pre-IND filing with the FDA by completing studies related to (1) specificity and potency assays to test TIL function; investigate changes in the T cell repertoire; and confirm that TILs do not become leukemic.

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Cell Therapy Market: Increasing use of human cells in cell therapy … – Digital Journal

PRESS RELEASE

Published March 10, 2023

According to the report, the global Cell Therapy Market is expected to grow from USD 21.6 Billion in 2022, which is expected to reach USD 78.80 Billion by 2033, growing at a CAGR of 14.15% from 2023 to 2033. Cell therapy is a type of medical treatment that involves the use of living cells to repair, replace, or regenerate damaged or diseased tissue in the body. The cells used in cell therapy may be sourced from the patients own body (autologous) or a donor (allogeneic) and can be obtained from various tissues, such as bone marrow, adipose tissue, or blood. The cells used in cell therapy can be either unmodified or genetically engineered to perform specific functions or to express therapeutic proteins. Some of the most common types of cells used in cell therapy include stem cells, immune cells, and cells that produce insulin. Cell therapy has been used to treat a wide range of conditions, including cancer, autoimmune diseases, neurological disorders, and cardiovascular diseases. The goal of cell therapy is to improve the bodys ability to repair and regenerate tissues, which can lead to better outcomes and improved quality of life for patients.

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Key Highlights:

Evolve Business Intelligence has published a new market research report on the Global Cell Therapy Market focusing on various aspects including market size and forecast, market dynamics, competitors market share analysis, market size in terms of value and volume, SWOT analysis, product benchmarking, key players recent developments, and opportunities, among others. The market has also been analyzed based on 4 indicators in the market dynamic chapter which include Drivers, Restraints, Key Trends, and Challenges. The overall sum of these sections will help to understand the best strategies to be adopted to prosper in this industry over the short and long terms. The quantitative analysis includes our authentic findings of this research study where we provided additional insight into what our readers can do to embrace new opportunities or plan against threats that might hinder the market.

The New Normal

As businesses recover from the COVID-19 pandemic, they have a new set of priorities to cope with. Throughout this crisis, they were forced to deal with shifting situations and often fell short of their ultimate goals. Now that a cure has been found and the pandemic is dying down, they will need to recuperate from this epidemic by setting forth new objectives to succeed in the future.

In terms of COVID-19 impact, the Cell Therapy Market report also includes the following data points:

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Key Players

Some of the major Cell Therapy Market players holding high market shares include Kolon TissueGene Inc, Osiris Therapeutics Inc, JCR Pharmaceuticals Co Ltd, PHARMICELL Co Ltd, and Castle Creek Biosciences Inc. These players use partnerships and new product development as key strategies to gain significant market share to compete with market leaders.

The key players profiled in the report are:

Segmental Analysis

Market Segment By Cell Type with a focus on market share, consumption trend, and growth rate of the Cell Therapy Market:

Market Segment By Therapy Type with a focus on market share, consumption trend, and growth rate of the Cell Therapy Market:

Market Segment By Therapeutic Area with a focus on market share, consumption trend, and growth rate of the Cell Therapy Market:

Market Segment By End User with a focus on market share, consumption trend, and growth rate of the Cell Therapy Market:

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Global Cell Therapy Market Geographic Coverage:

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