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How CAR-T Cell Therapy is Being Used in the Field of Blood Cancer Treatment – Curetoday.com

Currently only approved for the treatment of B-cell malignancies, CAR-T cell therapy is a relatively new treatment option that researchers are learning more about each day, according to Amelia Langston, MD.

Langston, a professor and Executive Vice Chair of the Department of hematology and medical oncology at the Winship Cancer Institute of Emory University, recently presented a session on CAR-T cell therapy at CURE's Educated Patient Leukemia & Lymphoma Summit, touching on not just how the treatment has evolved, but where it could be going in the near future.

Langston began by defining one of the major problems that researchers are trying to solve by utilizing CAR-T cell therapy: making immunotherapy a reality for more patients with blood cancer. There are a few methods that are currently in use, including allogeneic stem cell transplantation, monoclonal antibody therapy, and checkpoint inhibitors drugs that call upon a number of natural cellular immune responses.

At its core, the main concept of novel therapeutics is to bring the immune cells and the tumor cells together in a way that activates the immune cells to kill those cells that carry a specific target or targets. The goal is to also eliminate some of the complexities of the T-cell immune response and, according to Langston, potentially recruit other immune players into the mix.

The process of CAR-T cell therapy begins with T cells that are collected from a patient, Langston explained, and then manipulated in a laboratory. We then introduce a genetically engineered chimeric antigen receptor (CAR) construct that confers specificity for an antigen on the surface of the tumor cell. And that results in killing of the tumor.

It is worth noting, Langston said, that the manufacturing of these CAR-T cells is not trivial. Once the T cells are extracted, modified, and reproduced in large quantities, the patient then needs to be prepared with what doctors call lymphodepleting chemotherapy. This essentially makes room in the in the patient's immune system for the new T cells to get into the body and expand. After thats done, the T cells are delivered to the patient, where they expand inside the patient and get to work.

The first target that has been successfully applied to CAR-T cell therapy is a molecule called CD19, Langston went on to explain. This is a very attractive target because it corresponds to many of the different B-cell malignancies, with three different options currently approved by the FDA:

While it is an attractive option given its efficacy, CAR-T cell therapy comes with significant and unique toxicities, according to Langston namely, cytokine release syndrome.

The cells divide very rapidly in the patient, and they create a sort of a cytokine storm as a consequence of that rapid division, she said. This so-called cytokine release syndrome is characterized by high fevers, hypotension, diffuse capillary leak and swelling, and is associated with a lot of inflammatory markers. And if this complication is not identified quickly and managed appropriately, this is an absolutely life-threatening complication. The bright spot in this potential side effect is that effective interventions to help manage it do exist, mainly in the form of Actemra (tocilizumab).

Neurologic toxicities can also arise as a result of CAR-T cell therapy, and can also be life-threatening. Patients could have anything from a little bit of trouble with their memory, all the way to seizures and brain swelling and even death, Langston said. However, she did note, this is a reversible complication if it is identified and treated very quickly.

A third issue that can occur is B-cell aplasia, where the manufactured CAR T-cells attack normal B-cells that also express CD19, putting patients at higher risk of developing infections. Like the other toxicities related to CAR-T cell therapy, this condition can be treated as well, with intravenous immunoglobulin replacement therapy.

Citing CAR-T cell therapy success stories like that of Emily Whitehead, Langston concluded with a look at not just how this treatment can be refined and made more effective for patients, but what impact it could have on malignancies outside of the blood cancer space.

I think one of the most important areas (we are looking at) is how do we make the CAR-T cell concept work better? Langston asked. Can we add other immune modulators and stimulants into the mix to try to enhance persistence of those CAR-T cells? What about the fact that we have patients with T-cells that are really pretty beaten up from their prior therapies? Can we rejuvenate their T-cells before they are transduced? Or after transduction for that matter, in order to make the CAR-T cells more robust and more powerful? Can we develop new CAR constructs that will enhance the activity without maximizing toxicity?

When it comes to solid tumors, Langston concluded, her own institution is one of many that is currently investigating the possibilities. We have an ongoing study (at Emory) in lung cancer, we have another study in head neck cancer, and were beginning to get into breast cancer as well, she said.

While its challenging to identify targets in these cancers, Langston said, there is hope. It becomes more challenging when we look at some of the solid cancers, but there's a lot of promising data in early phase studies to suggest that this will be possible in the future.

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How CAR-T Cell Therapy is Being Used in the Field of Blood Cancer Treatment - Curetoday.com

Cell Separation Beads Market to Witness Robust Expansion Throughout the Forecast Period 2018-2028 – The Daily Philadelphian

With 1000+ market research reports and 1 billion+ data points, Future Market Insights (FMI) serves each and every requirement of the clients operating in the global healthcare, pharmaceuticals, and medical device industries. FMI deploys digital intelligence solutions to offercompelling insights to report buyersthat help them in overcoming market challenges, especially at the time of a crisis. Our dedicated team of professionals performs an extensive survey for gathering accurate information associated with the market.

FMI, in its upcoming business report, elaborates the historical and current scenario of the global Cell Separation Beads market in terms of production, consumption, volume, and value. The report scrutinizes the market into various segments, regions and players on the basis of demand pattern and growth prospects.

Crucial information and forecast statistics covered in the Cell Separation Beads market report will arm both existing and emerging market players with necessary insights to craft long-term strategies as well as maintain business continuity during a crisis such as the ongoing COVID-19 pandemic.

COVID-19 Impact Analysis on Cell Separation Beads Market

The recent outbreak of the COVID-19 has turned the spotlight on the healthcare industry, and subsequently impacted the Cell Separation Beads market. Severe shortages of critical medical supplies and a rapid rise in number of COVID-19 cases have resulted into a revolution rather than evolution in the healthcare ecosystems. Consequently, the impact is noticeable in the Cell Separation Beads market.

Following governments measures, particularly social distancing norms and stay-at-home orders, doctors are delaying or postponing elective surgeries unless critical to prevent the spread of the virus to individuals with comorbidities or chronic conditions. Additionally, movement restrictions and supply chain disruptions have created a logistical nightmare for market players, leading to severe product shortages in the global marketplace.

The FMIs report includes an interesting chapter on preliminary impact of the COVID-19 on the Cell Separation Beads market. This allows both leading and emerging market players to understand the market scenario during a crisis and aids them in making sound decisions to gain a distinct competitive edge.

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Cell Separation Beads Market: Segmentation

Valuable information covered in the FMIs Cell Separation Beads market report has been segregated into key segments and sub-segments.

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Cell Separation Beads Market: Competition Analysis

The FMIs study presents a comprehensive analysis of global, regional, and country-level players active in the Cell Separation Beads market.Competitive information detailed in the Cell Separation Beads market report has been based on innovative product launches, distribution channels, local networks, industrial penetration, production methods, and revenue generation of each market player. Furthermore, growth strategies and mergers & acquisitions (M&A) activities associated with the players are enclosed in the Cell Separation Beads market report.

Key players covered in the report include:

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Cell Separation Beads Market to Witness Robust Expansion Throughout the Forecast Period 2018-2028 - The Daily Philadelphian

Election results for California Propositions 14, 18, 23, and 24 – FOX 11 Los Angeles

LOS ANGELES - Twelve statewide propositions appeared on the California ballot for the November 3 election and voters will decide what becomes law.Those measures cover an array of topics including stem cell research, primary voting for 17-year-olds, kidney dialysis clinics and data privacy rules.

RELATED:Election Results: California Propositions

California voters pass Proposition 14

Proposition 14 would allow California to sell $5.5 billion in general obligationbonds for continued stem cell and medical research. The measure builds on Prop 71 that was enacted by voters in 2004 to support stem cell research and authorized $3 billion in bonds. According to theLeague of Women Voters of California,almost all the funds from the sale of the bonds authorized by Prop 71 have been spent.

California voters rejectProposition 18

Proposition 18 would allow 17-year-olds to vote in primaries and special elections, provided they turn 18 by the November general election. At least 18 states and Washington, D.C., have adopted laws that let people younger than 18 vote in certain circumstances.

Advocates say any chance to get people in the habit of voting should be encouraged. Besides, they say, young people whose birthdays fall between the primary and the general election are at an unfair disadvantage.

The Election Integrity Project California, the main opponent to Proposition 18, says that 17-year-olds are still considered children under the law and have no business deciding elections.

California voters reject Proposition 23

Proposition 23 was placed on the ballot by unions that represent health care workers and has attracted more than $110 million in political spending to persuade voters.

Opponents, financed by dialysis clinic companies, say that under that mandate, between two and three doctors would be required at every facility because most are open at least 16 hours a day, creating a financial burden that could lead some clinics to close.

Proposition 23 is the second attempt by the unions to increase regulation of dialysis clinics in California, where DaVita Inc. and Fresenius Medical Care two of the countrys largest for-profit dialysis providers operate about three-quarters of the states dialysis market.

California voters pass Proposition 24

Proposition 24 would update a 2018 law that gave Californians the right to know what information companies collect about them online, the right to get that data deleted and the right to opt out of the sale of their personal information.

The new measure would triple the fines for companies that violate kids privacy or break laws on the collection and sale of childrens private information. It would create a dedicated state agency to enforce the new law, with an annual budget of $10 million.

It also aims to close some loopholes that proponents say companies like Facebook, Google and Spotify have exploited.

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Election results for California Propositions 14, 18, 23, and 24 - FOX 11 Los Angeles

FUJIFILM Cellular Dynamics and Lonza Agree to Expand the Availability and Use of Induced Pluripotent Stem Cell Technology – BioSpace

About Lonza

At Lonza, we combine technological innovation with world class manufacturing and process excellence. Together, these enable our customers to deliver their discoveries in the healthcare, preservation, and protection sectors.

We are a preferred global partner to the pharmaceutical, biotech and specialty ingredients markets. We work to prevent illness and promote a healthier world by enabling our customers to deliver innovative medicines that help treat or even cure a wide range of diseases. We also offer a broad range of microbial control solutions, which help to create and maintain a healthy environment.

Founded in 1897 in the Swiss Alps, Lonza today operates in 120 sites and offices in more than 35 countries. With approximately 15,500 full-time employees, we are built from high-performing teams and of individual employees who make a meaningful difference to our own business, as well as the communities in which we operate. The company generated sales of CHF 5.9 billion in 2019 with a CORE EBITDA of CHF 1.6 billion. Find out more atwww.lonza.comand follow us on Twitter @LonzaGroup or Facebook @LonzaGroupAG.

About FUJIFILM

FUJIFILM Cellular Dynamics, Inc. (FCDI) is a leading developer and manufacturer of human induced pluripotent stem cells (iPSCs) utilized in drug discovery and cell therapies. FCDI is using its expertise in iPSC technologies to develop a robust cell therapeutics pipeline to address unmet medical needs in areas such as age-related macular degeneration, retinitis pigmentosa and immuno-oncology. For its partners, FCDI utilizes its iPSC platform to advance the progress of therapeutic candidates in the clinic and provides contract development and manufacturing (CDMO) services. In addition to cell therapy, FCDI also offers life science research tools including the companys inventoried iCell products, which are available in almost any cell type and are sourced from multiple cell lines which can be applied for target identification as well as toxicity testing. The company also offers custom cell development services and cell banking. FCDIs goal is to leverage the vast utility of iPSCs to advance human health and improve the quality of life for patients around the world. For more information, please visit:https://fujifilmcdi.com

FUJIFILM Holdings Corporation, Tokyo, Japan, brings cutting edge solutions to a broad range of global industries by leveraging its depth of knowledge and fundamental technologies developed in its relentless pursuit of innovation. Its proprietary core technologies contribute to the various fields including healthcare, highly functional materials, document and imaging products. These products and services are based on its extensive portfolio of chemical, mechanical, optical, electronic and imaging technologies. For the year ended March 31, 2020, the company had global revenues of $21 billion, at an exchange rate of 109 yen to the dollar. Fujifilm is committed to responsible environmental stewardship and good corporate citizenship. For more information, please visit:www.fujifilmholdings.com

Lonza Contact Details

FUJIFILM Contact Details:

Christine Jackman

FUJIFILM

(914) 789-8523

christine.jackman@fujfilm.com

Additional Information and Disclaimer

Lonza Group Ltd has its headquarters in Basel, Switzerland, and is listed on the SIX Swiss Exchange. It has a secondary listing on the Singapore Exchange Securities Trading Limited (SGX-ST). Lonza Group Ltd is not subject to the SGX-STs continuing listing requirements but remains subject to Rules 217 and 751 of the SGX-ST Listing Manual.

Certain matters discussed in this news release may constitute forward-looking statements. These statements are based on current expectations and estimates of Lonza Group Ltd, although Lonza Group Ltd can give no assurance that these expectations and estimates will be achieved. Investors are cautioned that all forward-looking statements involve risks and uncertainty and are qualified in their entirety. The actual results may differ materially in the future from the forward-looking statements included in this news release due to various factors. Furthermore, except as otherwise required by law, Lonza Group Ltd disclaims any intention or obligation to update the statements contained in this newsrelease. All product and company names herein may be trademarks of their registered owners.

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FUJIFILM Cellular Dynamics and Lonza Agree to Expand the Availability and Use of Induced Pluripotent Stem Cell Technology - BioSpace

Cleveland Clinic team draws a link between COVID-19 protection and the sleep aid melatonin – FierceBiotech

The idea of repurposingexisting medicines as a fast approach to containing COVID-19 is still popular, even as vaccines and antibodies designed to combat the disease are starting to gain steam. Scientists at the Cleveland Clinic are among those examining existing compounds as possible treatments for the coronavirus, and now they're suggesting that the popular over-the-counter sleep aid melatonin may be a possible option in treating the disease.

The researchers used an artificial intelligence tool to analyze data from 26,779 individuals in the Cleveland Clinics COVID-19 registry, of whom 8,274 tested positive for SARS-CoV-2, the novel coronavirus that causes COVID-19.

They found that people who were taking melatonin were 28% less likely to test positive for SARS-CoV-2, after adjusting for factors such as age, sex and underlying diseases, according to results published in the journal PLOS Biology.

Much has been written about the promise of real-world data (RWD) in life sciences, but how does it work in practice? We address this question in a new whitepaper that demonstrates the potential benefits of new RWD technologies with a proof of concept study to show how RWD can be incorporated into clinical research.

The melatonin effect was more pronounced in African Americans, with a reduction of 52%. In White Americans, the number was 23%.

Melatonin is a hormone released by the body that regulates the sleep-wake cycle. As a dietary supplement, its commonly used to help manage insomnia andjet lag.

Besides melatonin, the Cleveland Clinic team also found that the beta-blocker carvedilol, sold under the brand Coreg for high blood pressure and other heart diseases, was associated with a 26% reduction in a persons chance of testing positive for SARS-CoV-2.

RELATED:Melatonin? Stem cells? Researchers step up with unconventional approaches to COVID-19

Some members of the same Cleveland Clinic team previously pinpointed melatonin among a group of drugs they suggested might work for COVID. They showed that melatonin and mercaptopurine might work as a good combo for COVID. Those findings came from a pharmacology-based platform that used a technique called network proximity analysis. It was based on the idea that some proteins involved in other diseases might hold proximity to a virus interaction with the host.

The researchersapplied the same method in the current study to shed a light on clinical manifestations and pathologies common between COVID-19 and 64 other diseases. Closer proximity would mean a higher likelihood of pathological associations between the diseases.

They found that proteins involved in respiratory distress syndrome and sepsis were highly connected with SARS-CoV-2. That wasnt a surprise given that the two disorders can also cause death in patients with severe COVID-19.

This signals to us that a drug already approved to treat these respiratory conditions may have some utility in also treating COVID-19 by acting on those shared biological targets, Feixiong Cheng, Ph.D., the studys senior author, said in a statement.

Overall, they identified close network proximity to SARS-CoV-2 proteins from inflammatory bowel disease, attention deficit hyperactivity disorder, as well as pulmonary diseases such as COPD. Using a computational model, they identified 34 drugs that were significantly proximal to two or more SARS-CoV-2 host protein sets.

RELATED:COVID-19: Bio researchers race to repurpose everything from antiviral to anticancer discoveries

A team at Columbia University has also linked melatonin with increased likelihood of clinical improvement among critically ill COVID-19 patients on intubation or mechanical ventilation.

The sleep-promoting supplement was also reportedly used by President Donald Trump during his COVID-19 infection, though its not clear if he was taking it specifically to treat the disease or as part of his daily nutrition routine.

Despite melatonin emerging as a top pick from the Cleveland Clinic registry, Cheng cautioned that larger, randomized control trials would be needed before the supplement could be widely adopted in the treatment of COVID-19.

Cheng added that AI-based approaches to analyzing COVID-19 patient registries should be embraced in the effort to find effective treatments for the disease.Recent studies suggest that COVID-19 is a systematic disease impacting multiple cell types, tissues and organs, so knowledge of the complex interplays between the virus and other diseases is key to understanding COVID-19-related complications and identifying repurposable drugs, Cheng said. Our study provides a powerful, integrative network medicine strategy to predict disease manifestations associated with COVID-19 and facilitate the search for an effective treatment.

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Cleveland Clinic team draws a link between COVID-19 protection and the sleep aid melatonin - FierceBiotech

Global Cancer Stem Cell Therapy Market Outlook by Types, Applications, End Users and Opportunities to 2025 – TechnoWeekly

The Cancer Stem Cell Therapy market analysis is provided for the international markets including development trends, competitive landscape analysis, and key regions development status. The report provides key statistics on the market status of the Cancer Stem Cell Therapy manufacturers and is a valuable source of guidance and direction for companies and individuals interested in the industry.

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The global Cancer Stem Cell Therapy market 2020 research is a professional and in-depth study on the current state of the industry and provides a basic overview of the industry including definitions, classifications, applications and industry chain structure. The Cancer Stem Cell Therapy market analysis is provided for the international markets including development trends, competitive landscape analysis, and key regions development status. Development policies and plans are discussed as well as manufacturing processes and cost structures are also analyzed. This report also states import/export consumption, supply and demand Figures, cost, price, revenue and gross margins.

This report presents the worldwide Cancer Stem Cell Therapy market size (value, production and consumption), splits the breakdown (data status 2015-2019 and forecast to 2025), by manufacturers, region, type and application. This study also analyzes the market status, market share, growth rate, future trends, market drivers, opportunities and challenges, risks and entry barriers, sales channels, distributors and Porters Five Forces Analysis.

Companies profiled and studied for this Cancer Stem Cell Therapy market report include AVIVA BioSciences, AdnaGen, Advanced Cell Diagnostics, Silicon Biosystems and others.

The Report is segmented by types Autologous Stem Cell Transplants, Allogeneic Stem Cell Transplants, Syngeneic Stem Cell Transplants, Others and by the applications Hospital, Clinic, Medical Research Institution, Others,.

The report focuses on global major leading industry players of Cancer Stem Cell Therapy market providing information such as company profiles, product picture and specification, capacity, production, price, cost, revenue and contact information. Upstream raw materials and equipment and downstream demand analysis is also carried out. The Cancer Stem Cell Therapy market development trends and marketing channels are analyzed. Finally the feasibility of new investment projects are assessed and overall research conclusions offered.

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Global Cancer Stem Cell Therapy Market Outlook by Types, Applications, End Users and Opportunities to 2025 - TechnoWeekly

As California Passes Prop 14, What Is Stem Cell Research and Why Is It Controversial? – Newsweek

Voters in California have approved Proposition 14, which will pump billions of dollars into the state's stem cell research program. The Associated Press called the vote on Thursday, with 51 percent of ballots for and 49 percent against.

The result will allow the state to borrow $5.5 billion from investors for its stem cell agency, the California Institute for Regenerative Medicine (CIRM). The moneywhich taxpayers will repay with interest over the next 30 yearswill enable the institute to stay open, expand its research programs, and build new facilities.

Some $1.5 billion of the money will be spent researching conditions affecting the brain and central nervous system, such as Alzheimer's, Parkinson's, epilepsy, and stroke.

Unlike specialized cellssuch as blood cells or bone cellsstem cells do not have a specific job. Think of them as the raw materials of our bodies. When they divide, they can either renew and make new stem cells, or turn into specialized cells.

Despite making headlines for years, stem cell research is still in its early stages, with some treatments that have appeared to have worked in animals now going into clinical trials. These include treatments for macular degeneration, a common cause of blindness, as well as stroke, Lou Gehrig's disease, and spinal cord injuries.

It is hoped growing stem cells into specialized cells could also one day be used to replace damaged tissue and organs, for instance by helping the pancreas produce insulin in people with diabetes.

Currently, stem bone marrow transplantation is the most common form of stem cell therapy, used to treat blood cancer patients. Stem cell therapy has also been used for grafts of corneal stem cells, as well as skin grafts for burns victims.

As well as creating treatments, stem cell research can also help scientists understand diseases. Observing the cells in a lab as they turn into specialized cells, for instance, can provide clues on how we develop certain conditions.

There are a number of stem cells: embryonic stem cells, adult stem cells, adult stem cells tweaked to behave like embryonic stem cells, and stem cells found in the amniotic fluid and the umbilical cord of babies.

The controversy around stem cell research largely lies in the use of embryonic stem cells. These are taken from human embryos in their early stages of development. Opponents have likened this to abortion, although others disagree with this stance.

Embryonic stem cells used in research come from donations from IVF clinics, where an egg is fertilized with a sperm but not implanted into a patient because it is not needed. Embryonic stem cells are preferred over adult stem cells, as it may not be possible to specialize the latter and they are more likely to have abnormalities. But research suggests that it may be possible to turn adult stem cells into a wider range of specialized cells than previously thought, which may make them more useful.

In 2001, the Bush administration banned federal funding for stem cell research. This lead real estate developer Robert N. Klein II to initiate and help fund Proposition 71 in California. The aim was to enshrine the right to carry out stem cell research in the state's constitution, and establish CIRM. Klein was motivated by his son's experience with Type 1 diabetes, and his mother's Alzheimer's diagnosis. In 2004, Californians voted in favor of the proposition.

The institute has performed 64 clinical trials, and published over 3,000 scientific articles on the subject. But 16 years after Proposition 71 passed, CIRM started to run out of funds, and stopped accepting applications for new projects last year. This prompted the Californians for Stem Cell Research, Treatments and Cures political action committee (PAC) to lead the campaign for Proposition 14. Klein was among its supporters, as well as California governor Gavin Newsom, LA mayor Eric Garcetti, and the Michael J. Fox Foundation established by the Back to the Future actor and Parkison's disease patient who is its namesake.

On November 1, the foundation urged people via Twitter to vote in favor of Prop 14 to fund research on neurological disease. "Without this proposition vital research may come to a halt, delaying new treatments for people with," it said.

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As California Passes Prop 14, What Is Stem Cell Research and Why Is It Controversial? - Newsweek

Organoids mimic the early development of the heart in mouse embryos – BioNews

16 November 2020

Organoids can be used to study early stages of heart development in mouse embryos, a new study shows.

Researchers from the cole Polytechnique Fdrale de Lausanne, Switzerland, have reported that they were able to produce a mouse heart organoid from embryonic stem cells, which displayed essential features of an early developing heart. They suggested that this reveals a novel application of organoids for studying early embryonic stages of development.

'One of the advantages of embryonic organoids is that, through the co-development of multiple tissues, they preserve crucial interactions that are necessary for embryonic organogenesis,' said Dr Giuliana Rossi, lead author of the study. 'The emerging cardiac cells are thus exposed to a context similar to the one that they encounter in the embryo.'

In their study, published in Cell Stem Cell, the team exposed mouse embryonic stem cells to a mix of three factors involved in promoting heart growth. One week later, the stem cells self-organised into so-called gastruloids:organoids with an embryo-like organisation, which displayed signs of early heart development. The cell aggregatesnot only expressed several genes known to regulate cardiovascular development, but also generated a structure resembling a vascular network. Furthermore, the researchers found an 'anterior cardiac crescent-like domain' in the gastruloids, which even produced a beating heart tissue. Similar to the muscle cells of the embryonic heart, this area was also sensitive to calcium ions.

Organoids have been mostly the focus of research into the generation of adult tissues and organs for pharmaceutical and medical research. In their new publication, Professor Matthias Ltolf and his team suggested that they can also provide a system to study early embryonic stages of the developing heart and other organs, as they preserve important tissue-tissue interactions.

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Organoids mimic the early development of the heart in mouse embryos - BioNews

Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS-CoV-2 infection – DocWire News

This article was originally published here

Stem Cells Transl Med. 2020 Nov 14. doi: 10.1002/sctm.20-0385. Online ahead of print.

ABSTRACT

Anti-inflammatory and immune-modulatory therapies have been proposed for the treatment of COVID-19 and its most serious complications. Among others, the use of mesenchymal stromal cells (MSCs) is under investigation given their well-documented anti-inflammatory and immunomodulatory properties. However, some critical issues regarding the possibility that MSCs could be infected by the virus have been raised. Angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) are the main host cell factors for the Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) entry but so far it is unclear if human MSCs express or do not these two proteins. To elucidate these important aspects, we evaluated if human MSCs from both fetal and adult tissues constitutively express ACE2 and TMPRSS2 and, most importantly, if they can be infected by SARS-CoV-2. We evaluated human MSCs derived from amnios, cord blood, cord tissue, adipose tissue and bone marrow. ACE2 and TMPRSS2 were expressed by the SARS-CoV-2-permissive human pulmonary Calu-3 cell line but not by all the MSCs tested. MSCs were then exposed to SARS-CoV-2 wild strain without evidence of cytopathic effect. Moreover, we also excluded that the MSCs could be infected without showing lytic effects since their conditioned medium after SARS-CoV-2 exposure did not contain viral particles. Our data, demonstrating that MSCs derived from different human tissues are not permissive to SARS-CoV-2 infection, support the safety of MSCs as potential therapy for COVID-19. AlphaMed Press 2020 SIGNIFICANCE STATEMENT: Human mesenchymal stromal cells (hMSCs) are currently under investigation for the treatment of COVID-19. However, the potential safety profile of hMSCs in this context has never been defined since none has described if they express ACE2 and TMPRSS2, the main host cell factors for SARS-CoV-2 entry, and if they can be infected by SARS-CoV-2. We provide the first evidence that ACE2 and TMPRSS2 are not expressed in hMSCs derived from both adult and fetal human tissues and, most importantly, that hMSCs are not permissive to SARS-CoV-2 infection. These results support the safety of MSCs as potential therapy for COVID-19.

PMID:33188579 | DOI:10.1002/sctm.20-0385

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Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS-CoV-2 infection - DocWire News

Cynata Therapeutics (ASX:CYP) begins phase three osteoarthritis trial – The Market Herald

Cynata Therapeutics (CPY) has begun its phase three osteoarthritis clinical trial using its Cymerus mesenchymal stem cell (MSC) product, CYP-004.

The trial is sponsored by the University of Sydney and is funded by an Australian Government National Health and Medical Research Council (NHMRC) grant.

It will take place at study centres in Sydney and Tasmania and will begin with an initial four patients who will be assessed over a four week period.

Once completed, the study will open for a wider enrolment.

The aim of the trial is to assess the effect of Cymerus MSCs on the knee joint structure over a two-year period in 440 patients with osteoarthritis in the area.

The trial will be led by the Florance and Cope Chair of Rheumatology and Professor of Medicine at the University of Sydney, Professor David Hunter.

It also includes Professor Changhai Ding from the University of Tasmania, Professor Stefan Lohmander from the Lund University in Sweden, and Dr Rachel O'Connor and Dr Xia Wang from the University of Sydney.

"There is now cure for osteoarthritis and current treatment options largely focus on alleviating pain rather than modifying the course of the underlying disease," Professor David stated.

"We are delighted to commence this trial, which is designed to evaluate the disease modifying potential of Cymerus MSCs. Consequently, we anticipate that it will be an enormously influential trial, with the potential to inform clinical practice guidelines globally," he added.

MSCs are adult stem cells found in a wide range of human tissue such as bone marrow, placenta and fat tissue.

They are multi-potent, which means they can produce more than one type of cell. For example, they can differentiate into cartilage cells, fat cells, and bone cells.

They have been shown to ease regeneration and effects on the immune system without relying on engraftment (when transplanted cells start to grow and make healthy cells).

Patients will receive either an intra-articular injection of Cymerus MSCs or a placebo three times during a one year period. This will then followed-up for a two year period post enrolment.

Cynata will measure the quality of life, assessment of pain, physical function, and the proportion of participants achieving patient-acceptable symptom state - which is the highest level in which patients consider themselves satisfied with treatment.

"This clinical trial aims to determine whether Cynata's proprietary Cymerus MSC technology is active in the setting of knee osteoarthritis," Chief Operating Officer Dr Killian Kelly said.

"We look forward to advancing this clinical trial to investigate the potential benefits our MSCs could have to treat osteoarthritis patients with this common and debilitating disease," he added.

Cynata has ended the day 6.55 per cent in the green, with shares trading for 89.5 cents each in a $98.38 million market cap.

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Cynata Therapeutics (ASX:CYP) begins phase three osteoarthritis trial - The Market Herald