Faculty members receive funding to advance stem cell research – UBC Faculty – UBC Faculty of Medicine

By Stephanie Chow | March 12, 2020

Three Faculty of Medicine researchers Drs. Zachary Laksman, Bruce Verchere and Tim Kieffer have collectively received more than $1.6M from the Stem Cell Network (SCN) to advance their work in stem cell and regenerative medicine research.

The SCN investment, which will advance research collaborations across the country, aims to translate stem cell-based therapies from bench to bedside for the benefit of all Canadians.

Dr. Zachary Laksman, Department of Medicine, Division of Cardiology

UBC Collaborators: Dr. Glen Tibbits, Dr. Liam Brunham, Dr. Francis Lynn, Dr. Shubhayan Sanatani

Project: Pipeline Towards Stem Cell Driven Personalized Medicine for Atrial Fibrillation

Dr. Bruce Verchere, Department of Pathology & Laboratory Medicine

UBC Collaborators: Dr. Francis Lynn, Dr. Megan Levings, Tim Kieffer, Dr. Dina Panagiotopoulos, Dr. Brad Hoffman

Project: Genetic Manipulation of hES-derived Insulin-producing Cells to Improve Graft Outcomes

Dr. Tim Kieffer, Department of Cellular & Physiological Sciences

UBC Collaborators: Dr. James Piret, Dr. Megan Levings

Project: A Bioprinted Insulin-Producing Device for Diabetes

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Faculty members receive funding to advance stem cell research - UBC Faculty - UBC Faculty of Medicine

New approach to speed up red blood cells generation in the lab – BusinessLine

Transfusion of red blood cells (RBCs) is a life-saving treatment for numerous conditions such as severe anaemia, injury-related trauma, supportive care in cardiovascular surgery, transplant surgery, pregnancy-related complications, solid malignancies and blood-related cancers.

However, blood banks particularly in developing countries often face a severe shortage of whole blood as well as components of blood like red blood cells.

Researchers across the world are exploring possibilities to generate RBCs outside the body (in vitro) from haematopoietic stem cells (HSCs). These HSCs have the capability to give rise to the different types of cells found in the blood. Various groups have been able to produce RBCs in the laboratory from HSCs.

However, the process takes a long time - around twenty-one days. The resources required to grow cells in the laboratory over such a long duration can be very expensive for generation of RBCs on a large scale for clinical purposes.

A team of researchers led by Dr. L. S. Limaye, ex-scientist at the Department of Biotechnologys National Centre for Cell Science (NCCS) at Pune have found a way to tackle the issue.

They have found that the process can be speeded up by adding a very low concentration of a small protein molecule called `transforming growth factor 1 (TGF-1), along with a hormone called `erythropoietin (EPO), to the growth medium. They could cut down the process time by three days.

Dr. Limaye noted that several tests to assess the quality of the cells formed, and examination of many of their characteristics, including physical appearance, revealed that the RBCs formed using this procedure were normal.

The findings are worthy of further exploration. Additional investigations based on the insights gained from these studies could help assess the relevance of using this approach for blood transfusions in the future. The researchers have published a report on their work in the journal, `Stem Cell Research and Therapy.

(India Science Wire)

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New approach to speed up red blood cells generation in the lab - BusinessLine

Animal Stem Cell Therapy Market 2020: Insights, Key Strategies, Innovative Trends and Forecast Research upto 2027 – News Times

The latest research report on the Animal Stem Cell Therapy Market published by Stratagem Market Insights offers a profound awareness of the various market dynamics like trends, drivers, the challenges, and opportunities. The report further elaborates on the micro and macro-economic elements that are predicted to shape the increase of the Animal Stem Cell Therapy market throughout the forecast period (2020-2027).

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The major manufacturers covered in this report:

Medivet Biologics LLC, VETSTEM BIOPHARMA, J-ARM, U.S. Stem Cell Inc, VetCell Therapeutics, Celavet Inc., Magellan Stem Cells, Kintaro Cells Power

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In terms of region, this research report covers almost all the major regions across the globe such as North America, Europe, South America, the Middle East, and Africa and the Asia Pacific. Europe and North America regions are anticipated to show an upward growth in the years to come. While Animal Stem Cell Therapy Market in Asia Pacific regions is likely to show remarkable growth during the forecasted period. Cutting edge technology and innovations are the most important traits of the North America region and thats the reason most of the time the US dominates the global markets. Animal Stem Cell Therapy Market in the South, America region is also expected to grow in the near future.

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Flow cytometry market expected to reach $6.36 billion by 2027 – European Pharmaceutical Review

New research suggests the flow cytometry sector will grow at a CAGR of 8.2 percent due to increased adoption and expanding uses.

Research suggests the global flow cytometry market will grow at a compound annual growth rate (CAGR) of 8.2 percent from 2019 to 2027, with the sector expected to reach a value of $6.36 billion by the end of this period.

The report by ResearchAndMarkets suggests the growth will be driven by increased adoption of flow cytometry techniques in research and academia and the expanding use of these systems for immunology and immuno-oncology research. The report also cites the evolving pipeline of stem cell research and adoption of recombinant DNA technologies for antibody production as other drivers of the growth.

According to the research, the fastest growing technology is expected to be bead-based flow cytometry, because of its procedural advantages over the cell-based techniques (eg, ELSIE or western blot). The primary benefits are that it can detect multiple analytes with high reproducibility, stability and speed.

Reagents and consumables accounted for the largest share of the flow cytometry market in 2019 and the report suggests these will continue to drive growth, with application-specific reagents and assays being essential to the users of flow cytometry.

The report reveals that drug discovery had the largest share of the market in terms of application in 2019, with uses at multiple stages of the process particularly due to the development and implementation of multi-parameter intracellular flow cytometric analysis. The report expects growth in this application will continue, due to its ability to simplifying cell analysis procedures.

Pharmaceutical and biotechnology companies were the largest end users of flow cytometry in 2019. The report indicates the rise of chronic conditions leading to the development of new drugs is driving R&D expenditure and growth of the flow cytometry market.

North America held the largest share of the global flow cytometry market in 2019, followed by Europe, Asia-Pacific, Latin America and the Middle East & Africa. This trend is likely to continue in the forecast period, says the research.

Key players expected to continue to be influential in the market include: Agilent Technologies, Thermo Fisher Scientific and bioMerieux S.A. (France).

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Flow cytometry market expected to reach $6.36 billion by 2027 - European Pharmaceutical Review

Aging eyes and the immune system – Science Magazine

A central promise of regenerative medicine is the ability to repair aged or diseased organs using stem cells (SCs). This approach will likely become an effective strategy for organ rejuvenation, holding the potential to increase human health by delaying age-related diseases (1). The successful translation of this scientific knowledge into clinical practice will require a better understanding of the basic mechanisms of aging, along with an integrated view of the process of tissue repair (1).

The advent of SC therapies, now progressing into clinical trials, has made clear the many challenges limiting the application of SCs to treat disease. Our duty, as scientists, is to anticipate such limitations and propose solutions to effectively deliver on the promise of regenerative medicine.

Degenerating tissues have difficulty engaging a regulated repair response that can support efficient cell engraftment and restoration of tissue function (2). This problem, which I encountered when trying to apply SC-based interventions to treat retinal disease, will likely be an important roadblock to the clinical application of regenerative medicine approaches in elderly patients, those most likely to benefit from such interventions. I therefore hypothesized that the inflammatory environment present in aged and diseased tissues would be a major roadblock for efficient repair and that finding immune modulators with the ability to resolve chronic inflammation and promote a prorepair environment would be an efficient approach to improve the success of SC-based therapies (2, 3).

Immune cells, as sources and targets of inflammatory signals, emerged naturally as an ideal target for intervention. I chose to focus on macrophages, which are immune cells of myeloid origin that exist in virtually every tissue of the human body and which are able to reversibly polarize into specific phenotypes, a property that is essential to coordinate tissue repair (3, 4).

If there is an integral immune modulatory component to the process of tissue repair that has evolved to support the healing of damaged tissues, then it should be possible to find strategies to harness this endogenous mechanism and improve regenerative therapies. Anchored in the idea that tissue damage responses are evolutionarily conserved (5), I started my research on this topic using the fruit fly Drosophila as a discovery system.

The fruit fly is equipped with an innate immune system, which is an important player in the process of tissue repair. Using a well-established model of tissue damage, I sought to determine which genes in immune cells are responsible for their prorepair activity. MANF (mesencephalic astrocyte-derived neurotrophic factor), a poorly characterized protein initially identified as a neurotrophic factor, emerged as a potential candidate (6). A series of genetic manipulations involving the silencing and overexpression of MANF and known interacting partners led me to the surprising discovery that, instead of behaving as a neurotrophic factor, MANF was operating as an autocrine immune modulator and that this activity was essential for its prorepair effects (2). Using a model of acute retinal damage in mice and in vitro models, I went on to show that this was an evolutionarily conserved mechanism and that MANF function could be harnessed to limit retinal damage elicited by multiple triggers, highlighting its potential for clinical application in the treatment of retinal disease (2).

Having discovered a new immune modulator that sustained endogenous tissue repair, I set out to test my initial hypothesis that this factor might be used to improve the success of SC-based therapies applied to a degenerating retina. Indeed, the low integration efficiency of replacement photoreceptors transplanted into congenitally blind mice could be fully restored to match the efficiency obtained in nondiseased mice by supplying MANF as a co-adjuvant with the transplants (2). This intervention improved restoration of visual function in treated mice, supporting the utility of this approach in the clinic (7).

Next, my colleagues and I decided to address the question of whether the immune modulatory mechanism described above was relevant for aging biology and whether we could harness its potential to extend health span. We found that MANF levels are systemically decreased in aged flies, mice, and humans. Genetic manipulation of MANF expression in flies and mice revealed that MANF is necessary to limit age-related inflammation and maintain tissue homeostasis in young organisms. Using heterochronic parabiosis, an experimental paradigm that involves the surgical joining of the circulatory systems of young and old mice, we established that MANF is one of the circulatory factors responsible for the rejuvenating effects of young blood. Finally, we showed that pharmacologic interventions involving systemic delivery of MANF protein to old mice are effective therapeutic approaches to reverse several hallmarks of tissue aging (8).

A confocal fluorescence microscope image of a giant macrophage shows MANF (mesencephalic astrocyte-derived neurotrophic factor) expression in red.

The biological process of aging is multifactorial, necessitating combined and integrated interventions that can simultaneously target several of the underlying problems (9). The potential of immune modulatory interventions as rejuvenating strategies is emerging and requires a deeper understanding of its underlying molecular and cellular mechanisms.

One expected outcome of reestablishing a regulated inflammatory response is the optimization of tissue repair capacity that naturally decreases during aging (3). Combining these interventions with SCbased therapeutics holds potential to deliver on the promise of regenerative medicine as a path to rejuvenation (1).

PHOTO: COURTESY OF J. NEVES

GRAND PRIZE WINNER

Joana Neves

Joana Neves received undergraduate degrees from NOVA University in Lisbon and a Ph.D. from the Pompeu Fabra University in Barcelona. After completing her postdoctoral fellowship at the Buck Institute for Research on Aging in California, Neves started her lab in the Instituto de Medicina Molecular (iMM) at the Faculty of Medicine, University of Lisbon in 2019. Her research uses fly and mouse models to understand the immune modulatory component of tissue repair and develop stem cellbased therapies for age-related disease.

PHOTO: COURTESY OF A. SHARMA

FINALIST

Arun Sharma

Arun Sharma received his undergraduate degree from Duke University and a Ph.D. from Stanford University. Having completed a postdoctoral fellowship at the Harvard Medical School, Sharma is now a senior research fellow jointly appointed at the Smidt Heart Institute and Board of Governors Regenerative Medicine Institute at the Cedars-Sinai Medical Center in Los Angeles. His research seeks to develop in vitro platforms for cardiovascular disease modeling and drug cardiotoxicity assessment. http://www.sciencemag.org/content/367/6483/1206.1

FINALIST

Adam C. Wilkinson

Adam C. Wilkinson received his undergraduate degree from the University of Oxford and a Ph.D. from the University of Cambridge. He is currently completing his postdoctoral fellowship at the Institute for Stem Cell Biology and Regenerative Medicine at Stanford University, where he is studying normal and malignant hematopoietic stem cell biology with the aim of identifying new biological mechanisms underlying hematological diseases and improving the diagnosis and treatment of these disorders. http://www.sciencemag.org/content/367/6483/1206.2

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Aging eyes and the immune system - Science Magazine

Back to the drawing board for triple-negative breast cancer targets, researchers propose new combo approach – Endpoints News

The reason why triple-negative breast cancer is such a tough disease to treat is largely given away in its name. Such tumors cant be defined by traditional biomarkers neither estrogen receptors, progesterone receptors, nor excess HER2 protein forcing drug hunters down uncharted new pathways.

Researchers at Vanderbilt-Ingram Cancer Center explored one of them, and turned up with some new suggestions.

In a new paper, the scientists began with the observation that deregulated MYCN a member of the transcription factor family that activates expression of some oncogenes has been implicated in a subset of breast cancers with unfavorable prognostic features and clinical outcomes. They ended by putting forth a new drug regimen that could spark new hope.

Given that patients with TNBC primarily receive systemic cytotoxic chemotherapies that frequently result in unfavorable outcomes, they wrote in Science Translational Medicine, we propose the clinical development of combination BETi and MEKi for patients with advanced TNBC, with parallel evaluation of MYCN as a potential marker for patient selection.

Johanna Schafer, a graduate student working in Jennifer Pietenpols lab, is the first author, while the professor is the senior author.

The MYCN protein, sometimes dubbed N-Myc, has long been studied as a target in neuronal or neuroendocrine tumors, but its role in breast cancer is less clear. Its distinct from MYC (c-Myc), though the two are believed to affect each other.

Their intricate relationship would prove crucial in therapeutic development. But the first question is just how common they are, and according to the study, the two family members are heterogeneously expressed in separate cell nuclei within a given tumor in at least 40% of TNBC tumors. In fact, the expression of MYCN appeared to increase after neoadjuvant chemotherapy, part of the current standard of care.

The prevalence gave them enough reason to think about how to target it. When the team selected a cell line model, they had another finding that MYCN-expressing cells were essentially more prone to resistance to PI3K inhibitors, which block an alternative pathway for tumor growth.

Because the MYC family lack catalytic domains, the team resorted to epigenetic regulators, screening 158 compounds against the cell lines. BET drugs, which block the bromodomain (BRD)-containing family of transcriptional regulators, emerged as the winner.

It echoes an earlier study, done at Michigan State University, showing that the experimental class of molecules can prevent the growth of breast and lung cancers.

But thats not it and heres where the MEK inhibitors come in.

Most of the MYNC-expressing TNBCs also contain MYC-expressing cells, the researchers noted, which can still drive cancer growth. In fact, single-agent treatment with a BETi seemed to have increased MYC expression. Adding trametinib (Mekinist) to the cells, however, decreased the amount of both proteins. The results were further tested and confirmed in mouse models.

As a next step, our research team is proposing the further development and clinical trials of this combination therapy, Pietenpol, the director of Vanderbilt-Ingram and EVP for research at Vanderbilt University Medical Center, said in a statement.

Incyte, which has a pact in place to fund Vanderbilt research such as this study, has a BET inhibitor in early development.

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Back to the drawing board for triple-negative breast cancer targets, researchers propose new combo approach - Endpoints News

Stem Cell Reconstructive Market 2020 By Top Key Players/Manufacturers, Type and Application, Regions, Industry Analysis, Growth, Size, Trends and…

The latest research report on the Stem Cell Reconstructive Market published by Verified Market Research provides a profound awareness of the various market dynamics such as Trends, drivers, challenges and opportunities. The report explains in more detail the micro and macroeconomic elements that are expected to influence the growth of the Stem Cell Reconstructive Market over the forecast period (2020-2026).

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TAGS: Stem Cell Reconstructive Market Size, Stem Cell Reconstructive Market Growth, Stem Cell Reconstructive Market Forecast, Stem Cell Reconstructive Market Analysis, Stem Cell Reconstructive Market Trends, Stem Cell Reconstructive Market

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Stem Cell Reconstructive Market 2020 By Top Key Players/Manufacturers, Type and Application, Regions, Industry Analysis, Growth, Size, Trends and...

Stem Cell Banking-Market Market Strategies and Insight Driven Transformation 2019-2025 – Times Plot

An analysis of Stem Cell Banking-Market Market has been provided in the latest report launched by UpMarketResearch.com that primarily focuses on the market trends, demand spectrum, and future prospects of this industry over the forecast period. Furthermore, the report provides a detailed statistical overview in terms of trends outlining the geographical opportunities and contributions by prominent industry share contenders.

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Moreover, the report centers on providing comprehensive analytical data on the regional segments, which include North America, Asia-Pacific, Middle East& Africa, and the Rest of the World. Other than this, development plans & policies, marketing terminologies, manufacturing protocols, current trends, dynamics of the market, and classification have been explained in brief in this report. The team of researchers and analysts presents the readers accurate statistics and analytical data in the report in a simple manner by means of graphs, diagrams, pie charts, and other pictorial illustrations.

Major Players included in this report are as follows CCBCCBRViaCordEsperiteVcanbioBoyalifeLifeCellCrioestaminalRMS RegrowCordlife GroupPBKM FamiCordCells4lifeBeikebiotechStemCyteCryo-cellCellsafe Biotech GroupPacifiCordAmericordKrioFamilycordCryo StemcellStemade Biotech

Stem Cell Banking-Market Market can be segmented into Product Types as Umbilical Cord Blood Stem CellEmbryonic Stem CellAdult Stem CellOther

Stem Cell Banking-Market Market can be segmented into Applications as Diseases TherapyHealthcare

Stem Cell Banking-Market Market: Regional analysis includes: Asia-Pacific (Vietnam, China, Malaysia, Japan, Philippines, Korea, Thailand, India, Indonesia, and Australia) Europe (Turkey, Germany, Russia UK, Italy, France, etc.) North America (United States, Mexico, and Canada.) South America (Brazil etc.) The Middle East and Africa (GCC Countries and Egypt.)

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Introduction about Global Stem Cell Banking-Market Market Global Stem Cell Banking-Market Market Size (Sales) Market Share in 2019 by Product Type (Categorization) Global Stem Cell Banking-Market Market Size (Sales) Market Share in 2019 by Application Type (End-Users) Global Stem Cell Banking-Market Growth Rate and Sales (2019-2025) Global Stem Cell Banking-Market Market Share and Sales (Volume) Comparison by Applications Global Stem Cell Banking-Market Suppliers/Players Profiles along with their Sales Data Stem Cell Banking-Market Competition by Region, Application, Type, and Suppliers/Players Defined (Value, Sales Price, and Volume) table for each geographic region under Stem Cell Banking-Market A separate table of product value, market sales, gross margin, and revenue (2014-2019) for each product type

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Stem Cell Banking-Market Market Strategies and Insight Driven Transformation 2019-2025 - Times Plot

Stem Cell Alopecia Treatment Market Overview, Growth Opportunities, Industry Analysis, Size, Strategies and Forecast to 2026 – NJ MMA News

Verified Market Research has released a current and up-to-date Stem Cell Alopecia Treatment Market report that provides a detailed assessment of the value chain, a comprehensive study of market dynamics including drivers, constraints and opportunities, current trends, and industry performance analysis. In addition, critical aspects of key issues such as market competition, regional growth and market segmentation are examined in detail so that readers can gain a thorough understanding of the Stem Cell Alopecia Treatment market.

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Table of Content (TOC):

1 Stem Cell Alopecia Treatment Market Overview

2 Stem Cell Alopecia Treatment Market Competition by Manufacturers

3 Stem Cell Alopecia Treatment Production Market Share by Regions

4 Stem Cell Alopecia Treatment Market Consumption by Regions

5 Stem Cell Alopecia Treatment Production, Revenue, Price Trend by Type

6 Stem Cell Alopecia Treatment Market Analysis by Applications

7 Company Profiles and Key Figures in Stem Cell Alopecia Treatment Business

8 Stem Cell Alopecia Treatment Business Cost Analysis

9 Marketing Channel, Suppliers and Customers

10 Market Dynamics

11 Stem Cell Alopecia Treatment Market Forecast

12 Research Findings and Conclusion

13 Methodology and Data Source

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