Artificial Blood Substitutes Market Overview, Analysis, Trends, Size, Outlook and Forecast to 2028 – News Parents

Artificial Blood Substitutes Market: Overview

The global artificial blood substitutes market is predicted to register stellar growth rate in the forthcoming years. The presence of a large patient population that requires blood transfusion during surgeries, trauma, and for other blood disorders, which remains unmet due to shortage of blood supply has necessitated creation of artificial blood substitutes.

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Artificial blood substitutes are primarily used to mimic oxygen carrying capacity of biological blood and expand the blood volume in the human body. Use of artificial blood substitutes is at present considered an alternate method for blood transfusion. Further research is underway to develop more alternate methods for blood transfusion, including developing human red blood cells (RBCs) from stem cells of donors blood.

The research report provides an in-depth analysis of the artificial blood substitutes market over the forecast period. The report covers each and every key aspect pertinent to the market, including market dynamics, segmentation, and competitive scenario. The assessment of artificial blood substitutes market presented herein could serve as a valuable guide for both existing market participants, and the ones seeking entry in this market.

Artificial Blood Substitutes Market: Competitive Landscape and Notable Developments

The initial clinical trials for blood substitutes are recorded as early as early 1600, wherein milk, beer, urine, sheeps blood, and perfluorochemicals were administered as blood substitutes for animal and human subjects.

In successive periods, clinical trials of milk transfusion, including goats milk in large quantities were carried out but in vain. Clinical trials also involved injecting human milk that were futile too, which led researchers concede human milk not to be a substitute for blood.

With continual extensive research, over long periods, scientists have attained some success to develop blood substitutes. Artificial blood thus far developed can substitute red blood cells. While biological human blood performs several different functions, artificial blood performs the sole purpose of transporting oxygen and carbon dioxide in the body.

Established biotechnology companies in the ambit are engaged to develop blood substitutes. Such pursuits primarily involve developing oxygen carriers similar or above the capacity of biological blood. With concerted efforts of some top-notch biotechnology companies, namely HEMARINA SA, KaloCyte Inc. and Hemoglobin Oxygen Therapeutics LLC blood substitutes are available as oxygen carrier based on hemoglobin and perfluorocarbon-based oxygen carrier.

Nevertheless, presence of several well-established biotechnology companies engaged in the development of blood substitutes portrays a competitive yet moderately consolidated vendor landscape of the artificial blood substitutes market.

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Artificial Blood Substitutes Market: Key Trends

Worldwide, excessive blood loss due to traumatic injuries and diseases is responsible for vast number of deaths every year. Limited availability of fresh blood and small storage periods of fresh blood for such situations have necessitated development of artificial blood substitutes.

With continual experiments over long periods, scientists have thus far been able to create substitutes to mimic oxygen carrier capacity of biological blood. Development of perfluorochemical-based oxygen carrier and hemoglobin-based oxygen carrier and provide thrust to the artificial blood substitutes market.

Besides this, advent of stem cell therapy is poised to create new opportunities for demand of artificial blood substitutes.

However, on the downside, lower shelf life of artificial blood products and stringent regulatory approval process for these products restrain the growth of artificial blood substitutes market.

Artificial Blood Substitutes Market: Regional Outlook

North America is at the fore for demand within overall artificial blood substitutes market. Presence of advanced healthcare combined with awareness of individuals for alternate demonstrated therapies account for leading revenue share of the region.

Continual advances in stem cell therapy further indicates sustained growth of artificial blood substitutes market in the region.

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Missouri S&T researchers create organ tissue with bioactive glass, stem cells and 3D printer – Missouri S&T News and Research

An interdisciplinary team of Missouri S&T researchers is creating organ tissue samples using bioactive glass, stem cells and a 3D printer. The project could advance pharmaceutical testing and lead to a better understanding of how diseases affect human cells.

The researchers grow stem cells and add them to hydrogelsmade of alginate, gelatin or similar substances. Then, in a step unique toMissouri S&T, the researchers add bioactive glass to supply needed calciumions to the hydrogel/cell mixture and load the mixture as bioink into a 3Dprinter. They test the samples after bioprinting to determine the stem cellfunction, the materials tensile strength, degradation and the best glass typeto add.

Different cells prefer different gels, so we work to findwhich gel combination suits our research, says Dr. Krishna Kolan, apostdoctoral researcher at S&T. The challenge is that dissolved glass addscalcium, but it changes the pH, and cells need neutral pH to survive. Wefigured out which glass and how much to add to maintain neutral pH.

Kolan says researchers are several years away from making afunctioning organ, such as a liver or kidney, and the challenge is the vascularsystem and multiple types of cells in those organs. S&T researchers areworking on ways to develop vascular systems within the bioprinted tissue. Kolansays they can imbed a channel into engineered tissue during printing, then linethe channel with endothelial cells, which are the primary cells in bloodvessels. He is working on the experiments with two undergraduate students: AugustBindbeutel (mechanical engineering) and Lesa Steen (materials science andengineering).

Endothelial cells form networks in environments they like,such as glass-infused hydrogel, Kolan says. As the network grows, itvascularizes the tissue.

As researchers work toward someday repairing or replacingorgans with engineered organs, they are creating tissue models that can be usedfor pharmaceutical testing, Kolan says. Companies can scale down thecomposition of a drug to be appropriate for a tissue sample, he says. S&Tresearchers are also currently working on 3D-printed bone models. Biologygraduate student Bradley Bromet is comparing diseased cells with healthy stemcells to see in 3D how a disease diabetes, for instance affects cells.

Kolan isworking on the project with Dr. Ming Leu, the Bailey Professor in S&Tsmechanical and aerospace engineering department; Dr. Richard Brow, interimdeputy provost in the materials science and engineering department; Dr. DelbertDay, Curators Professor Emeritus of ceramic engineering, and Dr. Julie Semon, assistant professor ofbiology and director of S&Ts Laboratory of Regenerative Medicine.

Theresearch project showcases the types of research that complement the Universityof Missouri Systems NextGen Precision Health Initiative. NextGen is expectedto accelerate medical breakthroughs and improve lives by harnessing theresearch being done at the systems four universities and training a newgeneration of health scientists and practitioners.

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Single-cell Analysis Market Worth $5.6 Billion by 2025 – Exclusive Report by MarketsandMarkets – Yahoo Finance

CHICAGO, Feb. 21, 2020 /PRNewswire/ -- According to the new market research report "Single-cell Analysis Marketby Cell Type (Human, Animal, Microbial), Product (Consumables, Instruments), Technique (Flow Cytometry, NGS, PCR, Mass Spectrometry, Microscopy), Application (Research, Medical Application), End User - Global Forecasts to 2025", published by MarketsandMarkets, the Single-cell Analysis Marketis projected to reach USD 5.6 billion by 2025 from USD 2.1 billion in 2019, at a CAGR of 17.8% during the forecast period.

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The growth in this market is driven by technological advancements in single-cell analysis products, increasing government funding for cell-based research, growing biotechnology and biopharmaceutical industries, wide applications of single-cell analysis in cancer research, growing focus on personalized medicine, and the increasing incidence and prevalence of chronic and infectious diseases. However, the high cost of single-cell analysis products is expected to restrain the growth of this market to a certain extent during the forecast period.

The research applications segment accounted for the largest share of the market, by application, in 2018

Based on application, the Single-cell Analysis Market is segmented into research (cancer, immunology, neurology, stem cell, and other research applications) and medical applications (noninvasive prenatal diagnosis, in vitro fertilization, and circulating tumor cell detection). The research applications segment accounted for the largest share of the market in 2018. Increasing government initiatives in stem cell research and the wide usage of single-cell analysis in cancer research are the major factors driving the growth of the research applications segment.

Browsein-depth TOC on"Single-cell Analysis Market"

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The flow cytometry segment accounted for the largest market share in 2018

Based on technique, the market is segmented into flow cytometry, NGS, PCR, microscopy, mass spectrometry, and other techniques. The flow cytometry segment accounted for the largest market share in 2018. The large share of this segment is attributed to the wide usage of flow cytometry in detecting and measuring the physical and chemical characteristics of a population of cells or particles. However, the NGS segment is projected to register the highest growth rate during the forecast period. The high growth of the NGS segment is driven by the increasing application of single-cell analysis products in drug discovery for cancer and other chronic diseases.

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North America dominates the Single-cell Analysis Market

The global market is segmented into five major regions, namely, North America, Europe, Asia Pacific, Latin America, and the Middle East & Africa. In 2018, North America accounted for the largest share of the market. The growth in this market can be attributed to the increasing drug development activities in the pharmaceutical and biotechnology industries, rising prevalence of chronic and infectious diseases, and an increase in stem cell research activities.

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Prominent players in the Single-cell Analysis Market include Becton, Dickinson and Company (US), Danaher Corporation (US), Merck Millipore (US), QIAGEN (Netherlands), Thermo Fisher Scientific (US), General Electric Company (US), 10x Genomics (US), Promega Corporation (US), Illumina (US), Bio-Rad Laboratories (US), Fluidigm Corporation (US), Agilent Technologies (US), NanoString Technologies (US), Tecan Group (Switzerland), Sartorius AG (Germany), Luminex Corporation (US), Takara Bio (Japan), Fluxion Biosciences (US), Menarini Silicon Biosystems (Italy), and LumaCyte (US).

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Stem Cell Assay Market 2020| Analysis by Production, Consumption, Revenue and Growth Rate by 2026 – News Times

The Stem Cell Assay market was valued at XX Million US$ in 2018 and is projected to reach XX Million US$ by 2024, at a CAGR of XX% during the forecast period. In this study, 2018 has been considered as the base year and 2019 to 2024 as the forecast period to estimate the market size for Stem Cell Assay.Global Stem Cell Assay industry market professional research 2014-2024, is a report which provides the details about industry overview, industry chain, market size (sales, revenue, and growth rate), gross margin, major manufacturers, development trends and forecast.

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Key players in global Stem Cell Assay market include:

MerckThermo Fisher ScientificGE HealthcareAgilent TechnologiesBio-Rad LaboratoriesPromegaCell BiolabsPerkinElmerMiltenyi BiotecHemoGenixBio-TechneSTEMCELL

Market segmentation, by product types:

ViabilityPurificationIdentification

Market segmentation, by applications:

Regenerative MedicineClinical Research

Market segmentation, by regions:

North America (United States, Canada)Europe (Germany, France, UK, Italy, Russia, Spain)Asia Pacific (China, Japan, Korea, India, Australia, New Zealand)Middle East & Africa (Middle East, Africa)Latin America (Mexico, Brazil, C. America, Chile, Peru, Colombia)

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The report can answer the following questions:1. North America, Europe, Asia Pacific, Middle East & Africa, Latin America market size (sales, revenue and growth rate) of Stem Cell Assay industry.2. Global major manufacturers operating situation (sales, revenue, growth rate and gross margin) of Stem Cell Assay industry.3. Global major countries (United States, Canada, Germany, France, UK, Italy, Russia, Spain, China, Japan, Korea, India, Australia, New Zealand, Southeast Asia, Middle East, Africa, Mexico, Brazil, C. America, Chile, Peru, Colombia) market size (sales, revenue and growth rate) of Stem Cell Assay industry.4. Different types and applications of Stem Cell Assay industry, market share of each type and application by revenue.5. Global market size (sales, revenue) forecast by regions and countries from 2019 to 2024 of Stem Cell Assay industry.6. Upstream raw materials and manufacturing equipment, industry chain analysis of Stem Cell Assay industry.7. SWOT analysis of Stem Cell Assay industry.8. New Project Investment Feasibility Analysis of Stem Cell Assay industry.

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Stem Cell Assay Market 2020| Analysis by Production, Consumption, Revenue and Growth Rate by 2026 - News Times

Canine Stem Cell Therapy Market Regulations and Competitive Landscape Outlook to 2029 – Instant Tech News

Canine Stem Cell Therapy Market Research Study

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The report evaluates the current state of the Canine Stem Cell Therapy Market in terms of volume (X units), consumption, value (Mn/Bn), production and more. In addition, the study tracks the latest proceedings within the various market segments, end use industries, geographies, and regulatory landscape.

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Canine Stem Cell Therapy Market Regulations and Competitive Landscape Outlook to 2029 - Instant Tech News

Stem Cell Alopecia Treatment Market 2020 Company Profiles, Trends by Types and Application, Operating Business Segments 2026 – News Parents

New Jersey, United States, This report looks at the global market Stem Cell Alopecia Treatment Status and future trends, focusing on the global market major enterprises, while the current and future trends in North America, Europe, China, Asia Pacific and South America and other regions.

This article focuses on the market size, market share, market positioning, product type and development planning of enterprises with important roles in the global Market.

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The main companies operating in the Stem Cell Alopecia Treatment market are listed in the report.

In the segmentation section of the report, the authors have elaborately presented key driving factors for different segments of the global Stem Cell Alopecia Treatment industry. The report offers a Complete research study on product type and application segments of the global Stem Cell Alopecia Treatment industry. The segmental analysis provided in the report is expected to help players and investors to identify lucrative growth pockets of the global Stem Cell Alopecia Treatment industry.

The report offers a complete company profiling of leading players competing in the global Stem Cell Alopecia Treatment industry with high focus on share, gross margin, net profit, sales, product portfolio, new applications, recent developments, and several other factors. It also throws light on the vendor landscape to help players become aware of future competitive changes in the global Stem Cell Alopecia Treatment industry.

Global Stem Cell Alopecia Treatment Market: Regional Analysis

This part of the report includes detailed information of the market in different regions. Each region offers different scope to the market as each region has different government policy and other factors. The regions included in the report are North America, South America, Europe, Asia Pacific, and the Middle East. Information about different region helps the reader to understand global market better.

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Industry size and forecast: Industry analysts have provided historical, current and estimated forecasts of industry size in terms of value and volume.

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Industry trends and developments: Here the authors of the report talked about the most important trends and developments in the Stem Cell Alopecia Treatment market and its estimated effects on overall growth

Industry segmentation study: this part of the report provides a detailed breakdown of the main segments of the industry for Stem Cell Alopecia Treatment , including product type, application and industry.

Regional analysis: Stem Cell Alopecia Treatment providers receive important information about high-growth regions and their respective countries so that they can invest in profitable regions

Competitive landscape: This unit of the report highlights the competitive scenario of Stem Cell Alopecia Treatment by focusing on the key strategies of providers to consolidate their presence in the Stem Cell Alopecia Treatment business.

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Stem Cell Alopecia Treatment Market 2020 Company Profiles, Trends by Types and Application, Operating Business Segments 2026 - News Parents

Edinburgh BioQuarter reveals vision for expansion – Scottish Business News

EDINBURGH BioQuarter has released the first images of a 750m planned transformation which will create a new vibrant mixed-use neighbourhood, supporting a community of more than 20,000 people.

Withambitions to accelerate the development of BioQuarter and create EdinburghsHealth Innovation District, the long-term vision for the site now includesplans for residential housing. There will be more space for research andhealthcare innovation, commercial use, hotel, gym, retail and leisure. Theexpansion will support an estimated 9,000 new jobs, plus additional constructionjobs and to grow the pipeline of successful companies.

Over the last two decades, BioQuarter has played a pivotal role in cultivating world-leading medical research and life sciences innovation that is improving peoples lives around the world.

The 160-acre site, which lies three miles South of Edinburgh City Centre, is currently home to 8,000 people who work and study within its boundary and includes 13 award winning life sciences businesses based at BioQuarters Innovation Centre, the globally renowned Edinburgh Medical School, 900-bed Royal Infirmary of Edinburgh and one of Europes largest clusters of stem cell research at the Scottish Centre for Regenerative Medicine.

BioQuarterspartners City of Edinburgh Council, NHS Lothian, Scottish Enterprise and theUniversity of Edinburgh have already invested over 500m in the existingsite, with a further 300m investment planned in the next five years throughpipeline projects, including the new 68m University of Edinburgh UsherInstitute.

BioQuarteris developing a masterplan and place-making strategy and intends to seek ajoint venture partner through a formal OJEU process to accelerate developments.

Commenting on the vision for thenew BioQuarter, Hugh Edmiston, Chair of Edinburgh BioQuarter Strategy Board,said: This is the start of an exciting journey in the next chapter ofEdinburgh BioQuarters development. As we move ahead, we want to ensure that weinvolve our staff, students and the local communities in this long-term vision.

The opportunities here are vastand, once realised, can deliver significant and long lasting economic andsocial benefits for Edinburgh, Scotland and beyond.

Once completed the new visioncould deliver one of the largest single developments in the UK focussed on lifesciences innovation, health care delivery, medical research and teaching.

Interim Programme Director AnnaStamp said: We want to build on the success to date of theBioQuarter.Our plans are to create a vibrant new neighbourhood ofEdinburgh centred around a world-leading community of healthcare innovators. Bydeveloping at scale and at pace together with a private sector partner we willaccelerate solutions to global health challenges.

It is vital that BioQuarterdevelops as a welcome and accessible place for those who live around it, aswell as those who work or study here. We can do this by fostering a inclusiveenvironment which promotes wellbeing to create a welcoming place where peoplelive, learn, work, play, relax and discover as part of Edinburghs HealthInnovation District.

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Stem Cell manufacturing Market Competitive Strategies and Worldwide Demand with Top players- Thermo Fisher Scientific. Merck Group, Becton, Dickinson…

TheGlobal Stem Cell manufacturing Marketis expected to reach USD 16.51 Billion by 2025, from USD 10.28 Billion in 2017 growing at a CAGR of 6.1% during the forecast period of 2018 to 2025. The upcoming market report contains data for historic years 2017, the base year of calculation is 2017 and the forecast period is 2018 to 2025.

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The Global Stem Cell Manufacturing Market is highly fragmented and the major players have used various strategies such as new product launches, expansions, agreements, joint ventures, partnerships, acquisitions, and others to increase their footprints in this market. The report includes market shares of stem cell manufacturing market for global, Europe, North America, Asia Pacific and South America.

Major Market competitors/players:

Some of the major players operating in the stem cell manufacturing market are Thermo Fisher Scientific. Merck Group, Becton, Dickinson and Company. Holostem Advanced Therapies, JCR Pharmaceuticals, Organogenesis Inc, Osiris Therapeutics, Osiris Therapeutics, Vericel Corporation, AbbVie, American CryoStem, AM-Pharma, Anterogen.Co.,Ltd, Astellas Pharma, Bristol-Myers Squibb, Apceth Biopharma, Cellular Dynamics International, Rheacell, Takeda Pharmaceutical, Teva Pharmaceutical Industries Ltd. ViaCyte, VistaGen Therapeutics Inc, Translational Biosciences, GlaxoSmithKline plc, Daiichi Sankyo Company, Limited, among others.

Global Stem Cell manufacturing Market,By Application (Research Applicationsand Clinical Applicationsand Cell and Tissue Banking), By Product (Stem Cell Line, Instruments, Culture Media and Consumables), By End Users (Hospitals and Surgical Centers, Pharmaceutical and Biotechnology Companies, Clinics, Community Healthcare, Others), By Geography (North America, South America, Europe, Asia-Pacific, Middle East and Africa) Industry Trends and Forecast to 2025

Major Market Drivers:

Report Scope

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Circulating Tumor Cells (CTC) Market 2019 Analysis & Forecast To 2025 By Key Players, Share, Trend, Segmentation – News Parents

Global Circulating Tumor Cells (CTC) Market research report gives a comprehensive outlook of the markets 2019-2025 and offers an in-depth summary of the current market status, historic, and expected way forward for the Circulating Tumor Cells (CTC) Market. Additionally, to this, the report provides data on the restraints negatively impacting the markets growth. The report includes valuable information to assist new entrants, as well as established players, to understand the prevailing trends in the Market.

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Key Objectives of Circulating Tumor Cells (CTC) Market Report: Study of the annual revenues and market developments of the major players that supply Circulating Tumor Cells (CTC) Analysis of the demand for Circulating Tumor Cells (CTC) by component Assessment of future trends and growth of architecture in the Circulating Tumor Cells (CTC) Market Assessment of the Circulating Tumor Cells (CTC) Market with respect to the type of application Study of the market trends in various regions and countries, by component, of the Circulating Tumor Cells (CTC) Market Study of contracts and developments related to the Circulating Tumor Cells (CTC) Market by key players across different regions Finalization of overall market sizes by triangulating the supply-side data, which includes product developments, supply chain, and annual revenues of companies supplying Circulating Tumor Cells (CTC) across the globe

Major Players included in this report are as follows AdnaGenApocellBiocepCanopus BioscienceCreatv MicrotechIkonisysIV DiagnosticsMiltenyi BiotechNanostring TechnologiesRarecells DiagnosticsVitatex

Circulating Tumor Cells (CTC) Market can be segmented into Product Types as Ex-Vivo Positive SelectionIn-Vivo Positive SelectionNegative SelectionMicrochips & Single Spiral Micro Channel

Circulating Tumor Cells (CTC) Market can be segmented into Applications as Tumorigenesis ResearchEmt Biomarkers DevelopmentCancer Stem Cell ResearchOthers

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Circulating Tumor Cells (CTC) Market: Regional analysis includes: Asia-Pacific (Vietnam, China, Malaysia, Japan, Philippines, Korea, Thailand, India, Indonesia, and Australia) Europe (Turkey, Germany, Russia UK, Italy, France, etc.) North America (United States, Mexico, and Canada.) South America (Brazil etc.) The Middle East and Africa (GCC Countries and Egypt.)

Target Audience: Circulating Tumor Cells (CTC) Equipment Manufacturers Traders, Importers, and Exporters Raw Material Suppliers and Distributors Research and Consulting Firms Government and Research Organizations Associations and Industry Bodies

Stakeholders, marketing executives and business owners planning to refer a market research report can use this study to design their offerings and understand how competitors attract their potential customers and manage their supply and distribution channels. When tracking the trends researchers have made a conscious effort to analyse and interpret the consumer behaviour. Besides, the research helps product owners to understand the changes in culture, target market as well as brands so they can draw the attention of the potential customers more effectively.

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Report structure: In the recently published report, DataIntelo.com has provided a unique insight into the Circulating Tumor Cells (CTC) Industry over the forecasted period. The report has covered the significant aspects which are contributing to the growth of the global Circulating Tumor Cells (CTC) Market. The primary objective of this report is to highlight the various key market dynamics listed as drivers, trends, and restraints.

These market dynamics have the potential to impact the global Circulating Tumor Cells (CTC) Market. This report has provided the detailed information to the audience about the way Circulating Tumor Cells (CTC) industry has been heading since past few months and how it is going to take a shape in the years to come.

DataIntelo has offered a comprehensive analysis of the Circulating Tumor Cells (CTC) industry. The report has provided crucial information about the elements that are impacting and driving the sales of the Circulating Tumor Cells (CTC) Market. The section of competitive landscape keeps utmost importance in the reports published by DataIntelo. Competitive landscape section consists of key market players functioning in the worldwide industry of Circulating Tumor Cells (CTC).

The report has also analysed the changing trends in the industry. Several macroeconomic factors such as Gross domestic product (GDP) and the increasing inflation rate is expected to affect directly or indirectly in the development of the Circulating Tumor Cells (CTC) Market.

Table of Contents 1 Industry Overview of Circulating Tumor Cells (CTC) 2 Manufacturing Cost Structure Analysis 3 Development and Manufacturing Plants Analysis of Circulating Tumor Cells (CTC) 4 Key Figures of Major Manufacturers 5 Circulating Tumor Cells (CTC) Regional Market Analysis 6 Circulating Tumor Cells (CTC) Segment Market Analysis (by Type) 7 Circulating Tumor Cells (CTC) Segment Market Analysis (by Application) 8 Circulating Tumor Cells (CTC) Major Manufacturers Analysis 9 Development Trend of Analysis of Circulating Tumor Cells (CTC) Market 10 Marketing Channel 11 Market Dynamics 12 Conclusion 13 Appendix

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Circulating Tumor Cells (CTC) Market 2019 Analysis & Forecast To 2025 By Key Players, Share, Trend, Segmentation - News Parents

Visualizing the Conversion of Adult Cells to Stem Cells – Technology Networks

Researchers from the group of Vlad Cojocaru together with colleagues the Max Planck Institute in Mnster (Germany) have revealed how an essential protein helps to activate genomic DNA during the conversion of regular adult human cells into stem cells.A cells identity is driven by which DNA is read or not read at any point in time. Signaling in the cell to start or stop reading DNA happens through proteins called transcription factors. Identity changes happen naturally during development as cells transition from an undesignated cell to a specific cell type. As it turns out, these transitions can also be reversed. In 2012, Japanese researchers were awarded the Nobel prize for being the first to push a regular skin cell backwards to a stem cell.A fuller understanding of molecular processes towards stem cell therapiesUntil now, it is unknown how the conversion of a skin cell into a stem cell happens exactly, on a molecular scale. Fully understanding the processes with atomic details is essential if we want to produce such cells for individual patients in the future in a reliable and efficient manner, says research leader Vlad Cojocaru of the Hubrecht Institute. It is believed that such engineered cell types may in the future be part of the solution to diseases like Alzheimers and Parkinsons, but the production process would have to become more efficient and predictable.Pioneer transcription factorOne of the main proteins involved in the stem cell generation is a transcription factor called Oct4. It induces gene expression, or activity, of the proteins that reset the adult cell into a stem cell. Those genes induced are inactive in the adult cells and reside in tightly packed, closed states of chromatin, the structure that stores the DNA in the cell nucleus. Oct4 contributes to the opening of chromatin to allow for the expression of the genes. For this, Oct4 is known as a pioneer transcription factor.

The data from Cojocaru and his PhD candidate and first author of the publication Jan Huertas show how Oct4 binds to DNA on the so-called nucleosomes, the repetitive nuclear structures in chromatin. Cojocaru: We modelled Oct4 in different configurations. The molecule consists of two domains, only one of which is able to bind to a specific DNA sequence on the nucleosome in this phase of the process. With our simulations, we discovered which of those configurations are stable and how the dynamics of nucleosomes influence Oct4 binding. The models were validated by experiments performed by our colleagues Caitlin MacCarthy and Hans Schler in Mnster.One step closer to engineered factorsThis is the first time computer simulations show how a pioneer transcription factor binds to nucleosomes to open chromatin and regulate gene expression. Our computational approach for obtaining the Oct4 models can also be used to screen other transcription factors and to find out how they bind to nucleosomes, Cojocaru says.

Moreover, Cojocaru wants to refine the current Oct4 models to propose a final structure for the Oct4-nucleosome complex. For already almost 15 years now, we know that Oct4 together with three other pioneer factors transforms adult cells into stem cells. However, we still do not know how they go about. Experimental structure determination for such a system is very costly and time consuming. We aim to obtain one final model for the binding of Oct4 to the nucleosome by combining computer simulations with different lab experiments. Hopefully, our final model will give us the opportunity to engineer pioneer transcription factors for efficient and reliable production of stem cells and other cells needed in regenerative medicine.ReferenceHuertas et al. (2020) Nucleosomal DNA Dynamics Mediate Oct4 Pioneer Factor Binding. Biophysical Journal. DOI: https://doi.org/10.1016/j.bpj.2019.12.038

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Visualizing the Conversion of Adult Cells to Stem Cells - Technology Networks