Family honors child's memory through bone marrow registry and stem cell donation

Mark and Mindy Ammons lost their 2-year-old son, Christopher, in 1988 to neuroblastoma, an aggressive childhood cancer. In 2009, Mindy Ammons donated her own stem cells to a woman with cancer. And this weekend, the family's youngest son will prepare a bone marrow donor registry in memory of his oldest brother as an Eagle Scout project.

Family photo

Bone marrow donation is close to the heart for the Ammons family of Provo.

Mark and Mindy Ammons lost their 2-year-old son, Christopher, in 1988 to neuroblastoma, an aggressive childhood cancer. In 2009, Mindy Ammons donated her own stem cells to a woman with cancer. And this weekend, the familys youngest son will lead a bone marrow registry drive as an Eagle Scout project in memory of his oldest brother.

We are in the unique position of having been on both sides of the process, Mindy Ammons said.

In the "Be The Match" flier created for the project, Will Ammons, 13, explains that Christophers only chance of survival was a bone marrow transplant, but sadly, no one in our family was a match, so he had to be his own donor.

Christopher underwent treatment at the UCLA Medical Center where, after five days of chemotherapy, three days of full-body radiation and then surgery, he received his own marrow as a transplant. He died two weeks into the process, just shy of his third birthday.

Over the years, the Ammonses talked about this experience with their children and stayed informed on treatment advances. When it came time for their second oldest son, Jon, to do his Eagle Scout project, he didn't just want to do something to check off on a list. He wanted a meaningful project.

He wanted to do something that would make a difference and was cancer-related," Mindy Ammons said.

They discussed raising money for cancer research but decided that would be like dropping a coin in a well, she said.

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New Valley Medical program collects umbilical cord blood

UW Medicine/Valley Medical Center and Puget Sound Blood Center (PSBC) have joined together in a program to collect umbilical cord blood from new mothers who have just given birth. Cord blood is an important source of stem cells that can be used in lifesaving cancer treatment and research.

At the birth center here at Valley Medical Center, we have always been dedicated to providing comprehensive, high quality healthcare for the community we serve, said Judy Roudebush, vice president of Womens and Childrens Services at Valley Medical Center, in a press release. This new partnership between Valley and PSBC will help us to continue to expand care options for our community.

Cord blood is the blood remaining in the umbilical cord and placenta after the birth of a baby. Once considered a waste product that was discarded, umbilical cord blood is now known to be rich in life-saving hematopoietic stem cells the parent cells of all blood cells. About 150 milliliters of cord blood can be collected from each placenta and umbilical cord.

This program provides new moms with an opportunity to save lives at the same time they are bringing a new life into the world, remarked Donna Russell, principal at Donna Russell Consulting, LLC and Cchair of the Valley Medical Center Board of Trustees.

Cord blood collection is a painless procedure that does not interfere with the birth, or with mother-and-child bonding following delivery. There is no risk to either the mother or baby, and no cost associated with the donation. Families interested in donating cord blood at Valley Medical Center have several options for enrollment, depending on the babys due date.

We welcome this opportunity to partner with healthcare providers at Valley Medical Center to introduce a new on-site cord blood collection program, said Dr. James P. AuBuchon, president and CEO of PSBC. Cord blood is an important source for stem cell transplants that can be used to treat patients with leukemia, lymphoma and some metabolic or immune system disorders.

Families interested in donating cord blood should speak to their healthcare providers about how to make arrangements.

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New Valley Medical program collects umbilical cord blood

UCLA Gene Discovery Shows How Stem Cells Can Be Activated to Help Immune System Respond to Infection

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Newswise In a study led by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research member Dr. Julian Martinez-Agosto, UCLA scientists have shown that two genes not previously known to be involved with the immune system play a crucial role in how progenitor stem cells are activated to fight infection. This discovery lays the groundwork for a better understanding of the role progenitor cells can play in immune system response and could lead to the development of more effective therapies for a wide range of diseases.

The two-year study was published online October 30, 2014 ahead of print in the journal Current Biology.

Progenitor cells are the link between stem cells and fully differentiated cells of the blood system, tissues and organs. This maturation process, known as differentiation, is determined in part by the original environment that the progenitor cell came from, called the niche. Many of these progenitors are maintained in a quiescent state or "standby mode" and are ready to differentiate in response to immune challenges (such as stress, infection or disease).

Dr. Gabriel Ferguson, a postdoctoral fellow in the lab of Dr. Martinez-Agosto and first author of the study, built upon the lab's previous research that utilized the blood system of the fruit fly species Drosophila, showing that a specific set of signals must be received by progenitor cells to activate their differentiation into cells that can work to fight infection after injury. Dr. Ferguson focused on two genes previously identified in stem cells but not in the blood system, named Yorkie and Scalloped, and discovered that they are required in a newly characterized cell type called a lineage specifying cell. These cells then essentially work as a switch, sending the required signal to progenitor cells.

The researchers further discovered that when the progenitor cells did not receive the required signal, the fly would not make the mature cells required to fight infection. This indicates that the ability of the blood system to fight outside infection and other pathogens is directly related to the signals sent by this new cell type.

"The beauty of this study is that we now have a system in which we can investigate how a signaling cell uses these two genes Yorkie and Scalloped, which have never before been shown in blood, to direct specific cells to be made," said Dr. Martinez-Agosto, associate professor of human genetics. "It can help us to eventually answer the question of how our body knows how to make specific cell types that can fight infection."

Drs. Martinez-Agosto and Ferguson and colleagues next hope that future studies will examine these genes beyond Drosophila and extend to mammalian models, and that the system will be used by the research community to study the role of the genes Yorkie and Scalloped in different niche environments.

"At a biochemical level, there is a lot of commonality between the molecular machinery in Drosophila and that in mice and humans," said Dr. Ferguson. "This study can further our shared understanding of how the microenvironment can regulate the differentiation and fate of a progenitor or stem cell."

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UCLA Gene Discovery Shows How Stem Cells Can Be Activated to Help Immune System Respond to Infection

First human stomach tissue grown in lab

US researchers generated functional, three-dimensional human stomach tissue to create miniature stomachs using pluripotent stem cells in a laboratory.

Human pluripotent stem cells can transform into any cell type in the body.

"Until this study, no one had generated gastric cells from human pluripotent stem cells (hPSCs)," said Jim Wells, principal investigator and a scientist at Cincinnati Children's Hospital Medical Center.

"In addition, we discovered how to promote formation of three-dimensional gastric tissue with complex architecture and cellular composition," Wells added.

"This first-time molecular generation of 3D human gastric organoids (hGOs) presents new opportunities for drug discovery, modelling early stages of stomach cancer and studying some of the underpinnings of obesity related diabetes," Wells said.

Differences between species in the embryonic development and architecture of the adult stomach make mouse models less than optimal for studying human stomach development and disease, Wells pointed out.

The key to growing human gastric organoids was to identify the steps involved in normal stomach formation during embryonic development.

By manipulating these normal processes in a petri dish, the scientists were able to coax pluripotent stem cells into becoming stomach cells.

Over the course of a month, these steps resulted in the formation of 3D human gastric organoids that were about 3 mm (1/10th of an inch) in diameter.

The study appeared in the journal Nature.

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First human stomach tissue grown in lab

Human Stomach Made in the Lab Using Stem Cells

Using pluripotent stem cells researchers have been able to build a mini stomach in the lab.REUTERS

In a first, a miniature stomach was created in the lab by scientists using stem cells.

Pluripotent stem cells that can grow into any cell type were used by scientists at Cincinnati Children's Hospital Medical Center to generate the artificial stomach.

The scientists identified the steps in stomach formation in the human embryo and by manipulating these in a petri dishwere able to coax the stem cells to form a mini stomachmeasuring 3 mm in diameter.

They then studied how h.pylori bacteria affected stomach tissues and spread rapidly. The bacteria is responsible for peptic ulcer and stomach cancer.

This first-time molecular generation of a 3D human stomach (called gastric organoid) presents new opportunities for drug discovery, modelling early stages of stomach cancer and studying some of the underpinnings of obesity related diabetes, according to Jim Wells, PhD, principal investigator and a scientist in the divisions of Developmental Biology and Endocrinology at Cincinnati Children's.

The work was conducted in collaboration with researchers at the University of Cincinnati College of Medicine.

The discovery of how to promote formation of three-dimensional gastric tissue with complex architecture and cellular composition is important as mouse models are sometimes not the best fit when studying human ailments, the team said.

The human gastric organoids will be useful to identify biochemical processes in the gut that allow gastric-bypass patients to become diabetes-free soon after surgery before losing significant weight.

Obesity fuelled diabetes and metabolic syndrome are public health challenges, addressing which has been difficult due to lack of reliable laboratory modelling systems.

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Human Stomach Made in the Lab Using Stem Cells

Scientists grow miniature human stomachs from stem cells

A CT scan of a human abdomen with stomach cancer. Photograph: Bojan Fatur/Getty Images

Scientists have grown miniature human stomachs from stem cells as a way of studying gastric diseases such as ulcers and stomach cancer and in the future creating tissue to repair patients stomachs.

The mini-stomachs are grown in petri dishes from stem cells. Fully formed, they are the size of a pea and shaped like a rugby ball. They are hollow with an interior lining that is folded into glands and pits like a real stomach.

Crucially, the researchers found that the miniature stomachs, known as gastric organoids, respond to infection very much like ordinary human stomachs.

There hasnt been any good way to study human stomach disease before because animals just dont get the same diseases, said James Wells, director of the Pluripotent Stem Cell Facility, Cincinnati Childrens Hospital Medical Center, who led the research which is published in Nature.

Human gastric diseases are associated with chronic infection by the bacterium Helicobacter pylori. Half the worlds population is infected with the bug, which can be picked up from food. Although most people do not show symptoms, once the infection is present up to 20% of carriers will develop gastric ulcers during their lifetimes. Around 2% will develop stomach cancer.

In developing countries, where H. pylori infection is more prevalent, gastric cancers are the second leading cause of cancer-related deaths.

Having grown the mini-stomachs, the researchers then injected them with H. pylori. In animals, H. pylori has little effect and disease does not follow but in the gastric organoid, the invading bacteria behaved as if it were a real human stomach.

The bacteria began injecting their proteins into the surrounding cells, and started to multiply. This is the hallmark of infection, said Wells. We can now very effectively study the bacteria and how it generates diseases. This has never been possible before with human tissue in vitro.

This is not the first time that miniature organs have been grown from stem cells. In 2013, scientists grew miniature kidneys and successfully transplanted into a rat. Replacement windpipes, grown from stem cells on lab-made scaffolds, have also been grown and transplanted into patients.

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Scientists grow miniature human stomachs from stem cells

Mini-Stomachs Let Scientists Study Ulcers in a Lab Dish

Scientists have grown miniature stomachs in a lab dish using stem cells, and are already using them to study stomach cancer. They hope they can grow patches to fix ulcers, find new drugs to treat and even prevent stomach cancer, and perhaps even grow replacement stomachs some day.

They discovered that the bacteria that cause stomach cancer begin doing their dirty work almost immediately, attaching to the stomach lining and causing tumors to start growing in response. Helicobacter pylori causes many, if not most, cases of stomach cancer, which affects more than 22,000 Americans a year and kills half of them. Stomach cancer is a major killer globally, affecting close to a million people a year and killing more than 70 percent of them.

And the team grew their mini-stomachs using two different types of stem cells human embryonic stem cells, grown from very early human embryos, but also induced pluripotent stem cells or iPS cells, which are made by tricking bits of skin or other tissue into acting like a stem cell.

In our hands they worked exactly the same, James Wells of Cincinnati Childrens Hospital Medical Center, who led the research. Both were able to generate, in a petri dish, human stomach tissue.

Immunofluorescent image of human stomach tissue made using stem cells

Stem cells are the body's master cells. Embryonic stem cells and iPS cells are both pluripotent meaning they can give rise to any tissue in the body. They've been used to grow miniature human livers, retinas, brain tissue and have been injected into eyes to treat eye disease.

Growing anything close to a real stomach or even a patch for an ulcer is a long way off. The gastric organoids Wellss team made the name up are just about the size of a BB bullet.

Its not easy getting stem cells to do what you want them to do. Wells and his team, including graduate student Kyle McCracken, had to use various growth factors and chemicals, each introduced at precisely the right time, to coax the cells into becoming three-dimensional blobs of stomach tissue. The stomach is a complex organ, with layers of muscle cells, cells that make up the stomach lining and glands that secrete proteins and acid to digest food.

"The bacteria immediately know what to do and they behaved as if they were in the stomach.

But the process worked, and the mini-stomachs look just like stomach tissue, the team reports in this weeks issue of the journal Nature.

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Mini-Stomachs Let Scientists Study Ulcers in a Lab Dish

Stem Cells Used to Grow Mini-Stomachs Seeking Treatments

Researchers are using stem cells to grow tiny three-dimensional human stomachs that are structurally similar to the real thing, helping investigators seek treatments for gastric diseases such as ulcers and cancer.

Researchers carefully added growth hormones to embryonic or induced stem cells in a lab for as long as five weeks to encourage the development of gastric tissue, according to the findings published today in the journal Nature. The mini-stomachs, which even produce hormones that regulate the secretion of acid and digestive enzymes, may help discover therapies for diseases that affect as much as 10 percent of the worlds population.

The researchers are experimenting with tissue from the mini-stomachs to use as grafts for treating peptic ulcers, said James Wells, a professor of pediatrics at Cincinnati Childrens Hospital Medical Center in Ohio. Eventually they may be able to make larger organs that could be used for transplant, he said.

The transplant of a whole stomach is a way off, but its within a reasonable time frame to generate in a petri dish pieces of stomach to patch ulcers, Wells said in a telephone interview. There is no reason to think that if we can do this in miniature that we cant do it on a larger scale. This was a seminal step in that direction.

Some of the same investigators transplanted functioning human intestinal tissue grown from stem cells into mice, creating a model for studying intestinal diseases. Ultimately, tissue grown using a patients own stem cells may be used to treat their ailments, according to the study published last week in Nature Medicine.

The researchers are already able to use the tiny organs, about the size of a small green pea, to track the development of stomach ailments that are often caused by bacteria called Helicobacter pylori, Wells said. They inject the mini-stomachs with the bacteria and within hours they can see the cell replication it causes. One day they may be able to use the approach to see which experimental drugs block the damage.

The results may have more immediate impact on the production of lung and pancreatic cells that other researchers are crafting, he said. Those tissues are now grown on flat sheets, and using a three-dimensional approach may also work better for them, Wells said.

These are three-dimensional organs, he said. It makes sense to use a more functional approach.

To contact the reporter on this story: Michelle Fay Cortez in Minneapolis at mcortez@bloomberg.net

To contact the editors responsible for this story: Reg Gale at rgale5@bloomberg.net Andrew Pollack, Drew Armstrong

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The ethical, legal and political minefield of stem cell research

5 hours ago Mines. Credit: The District

Human stem cell research holds promise for combating some of the most recalcitrant of diseases and for regenerating damaged bodies. It is also an ethical, legal and political minefield.

Human stem cell research is a thriving field of science worldwide holding promise for treating diseases such as diabetes, multiple sclerosis and Parkinson's disease, as well as for furthering our understanding of how we develop from the very earliest stages of life.

But using human embryonic stem (ES) cells to improve the health of other humans has also been the subject of comment, criticism and even court cases. Time magazine dubbed the "complexity and drama" surrounding these cells as the "Great Debate".

Most notably, the field witnessed the 2001 restriction on funding for ES cell research in the USA by President Bush and the lifting of the ban in 2009 by President Obama. Then in 2011, the Court of Justice of the European Union (CJEU) banned the patenting of inventions derived from human eggs or their equivalent on the basis that they were human embryos, the commercial exploitation of which "would be contrary to morality."

While religious bodies and green lobbyists use patent law to elevate the status of the embryo, scientists argue that doing so threatens research that might benefit the health of millions.

International law permits states to refuse patents where necessary to protect morality in their territory. "Yet, how does a patent examiner or a court assess whether an invention is immoral to the point that, unlike other inventions, it can't be patented? That is a particularly difficult question," said Dr Kathy Liddell from the Faculty of Law. "It is a conundrum that runs headlong into the complex intersection of law and morality, intellectual property and philosophy."

It is precisely this intersection that a new research centre in the Faculty will investigate. The new centre funded by the Hatton Trust and the WYNG Foundation will focus on medical law, ethics and policy relating to controversial issues such as patenting inventions involving DNA and body parts, the regulation of medical research and technologies, assisted reproduction and surrogacy, and the governance of 'big data' in the medical field, as well as the regulatory and legislative issues that stem cell research is likely to meet en route from the lab to the clinic.

"These areas need to be considered not as a post hoc rationalisation of events that have already happened, but alongside and ahead of technological advances," said Liddell, who is centrally involved in the new centre, as well as being Deputy Director of the Faculty's Centre for Intellectual Property and Law. "To complement the extraordinary science that is happening, we need to consider the ramifications of biomedical advances in a thorough and timely way."

Liddell's own research interests relate to the pathway that leads from the research bench to clinically effective treatments. She sees the law's role as facilitating and supporting this pathway in morally responsible ways.

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The ethical, legal and political minefield of stem cell research

Stem Cell Treatment Centers | BioRegeneration Integrated …

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The stem cell treatments provided at the BioRegeneration Integrated Medical Centre are there to help you overcome any medical problem you may be experiencing. Located in a beautiful setting, the Stem Cell Treatment Centers are the perfect place to recover from an illness and get back on your feet. We also provide intravenous nutrient therapy, in addition to the stem cell treatments to enhance the speed of recovery. A stem cell treatment has saved many lives in the past, and continues to provide hope to the hopeless.

The treatments offered by the center are truly novel and unlike that which is offered in many countries around the world. They provide many patients with the chance to greatly improve their health in the most unique way possible. Every year stem cell treatments are being approved for more illness. So far there is full approval for most orthopedic cases including arthritis, sports injuries, ligament tears and fracture non-unions. There is also approval for Diabetes, Critical Limb Ischemia, Erectile Dysfunction, Emphysema and Chronic Bronchitis. Many other illnesses are still in the trial phase and the results are encouraging.

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