Co-administration of aspirin and adipose-derived stem cell conditioned | NDT – Dove Medical Press

Adel Galeshi,1,2 Maryam Ghasemi-Kasman,3,4 Farideh Feizi,2,3 Nahid Davoodian,5,6 Leila Zare,7 Zeinab Abedian3

1Babol University of Medical Sciences, Babol, Iran; 2Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran; 3Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; 4Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; 5Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; 6Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; 7Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Correspondence: Maryam Ghasemi-KasmanHealth Research Institute, Babol University of Medical Sciences, PO Box 4136747176, Babol, IranTel +98 113 219 0557Fax +98 113 219 0557Email m.ghasemi@mubabol.ac.ir

Farideh FeiziDepartment of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, PO Box 4136747176, Babol, IranTel +98 113 219 2033Fax +98 113 219 9936Email faridehfeizi@yahoo.com

Introduction: Based on beneficial effects of aspirin and mesenchymal stem cells (MSCs) on myelin repair, in a preset study, effects of co-administration of aspirin and conditioned medium from adipose tissue-derived stem cells (ADSC-CM) on functional recovery of optic pathway, demyelination levels, and astrocytes activation were evaluated in a lysolecithin (LPC)-induced demyelination model of optic chiasm.Methods: LPC (1%, 2 L) was injected into the rat optic chiasm and animals underwent daily intraperitoneal (i.p.) injections of ADSCs-CM and oral gavage of aspirin at a dose of 25 mg/kg for 14 days post LPC injection. The conductivity of visual signals was assessed using visual evoked potential recordings (VEPs) before LPC injection and on days 7 and 14 post lesion. Immunostaining against PDGFR as oligodendrocyte precursor cells marker, MOG as mature myelin marker, and GFAP as astrocyte marker was performed on brain sections at day 14 post LPC injection. FluoroMyelin staining was also used to measure the extent of demyelination areas.Results: Our results showed that administration of ADSCs-CM and aspirin significantly reduced the latency of VEP waves in LPC receiving animals. In addition, demyelination levels and GFAP expressing cells were attenuated while the number of oligodendrocyte precursor cells significantly increased in rats treated with ADSCs-CM and aspirin.Conclusion: Overall, our results suggest that co-administration of ADSCs-CM and aspirin improves the functional recovery of optic pathway through amelioration of astrocyte activation and attenuation of demyelination level.

Keywords: lysolecithin, demyelination, optic chiasm, mesenchymal stem cells, conditioned medium, aspirin

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License.By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

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Revolutionary Cosmetic Skin and Sexual Wellness Treatments Are Coming to Central New Jersey – PRNewswire

EAST BRUNSWICK, N.J., Sept. 17, 2019 /PRNewswire/ -- Botox, dermal fillers, and sexual wellness treatments are among the new aesthetic treatment procedures available at IVIV Health and Aesthetics, a medical aesthetic practice located in the heart of central New Jersey.

IVIV Health and Aesthetics is owned and operated by Dr. Tanya Weissman, a board-certified physician with extensive experience working with PRP treatments, stem cell treatments, and Botox injections to help manage chronic pain and heal damaged tissues and tendons. Weissman was named by NJ TopDocs as a 2019 Top Doctor.

"We take a physician-first approach at IVIV Health and Aesthetics because we want our patients to know that their health and safety is our priority," Weissman said. "Patients feel reassured by our use of nurses and nurse practitioners, who are fully trained in the protocol required for these treatments, including drawing blood and injection safety."

This makes IVIV Health and Aesthetics one of very few establishments that offer such a variety of wellness services and most importantly have an experienced medical professional to ensure they are administered in a safe and comfortable fashion.

Weissman is joined by Dr. Aleksandra Novik, a nurse practitioner who brings experience in aesthetics, cardiology, endocrinology and physical medicine and rehabilitation to IVIV Health and Aesthetics.

"Dr. Novik takes pride in making sure patients feel their best and achieve natural-looking results," Weissman said. "We are thrilled to welcome her to the IVIV Health and Aesthetics family."

According to Weissman, adding Botox injections to the practice enables her and Novik to treat medical conditions as well as cosmetic treatments. Botox is a common treatment for hyperhidrosis (excessive sweating), migraines, and TMJ, as well as for cosmetic enhancement such as fine lines, wrinkles, and crow's feet.

"These injections can be a very effective treatment for patients seeking to control excessing sweating in the palms, feet, and underarms," Weissman said.

According to Weissman, many treatments at IVIV Health and Aesthetics, particularly platelet-rich plasma (PRP) aesthetic treatments, are designed to support and enhance the body's natural healing power. The sexual wellness treatments added to the medical spa's lineup of services use PRP rejuvenation to increase blood flow to the genital area in both men and women, which helps with performance, sensitivity, and satisfaction.

"With regenerative treatments, the body uses its own resources to heal itself by enhancing its natural healing processes," Weissman said. "Whenever the body detects injury, platelets rush to the site to help facilitate the healing process. Platelet-rich plasma is full of those same growth factors that enable your body to regenerate using its own characteristics, making it an excellent option for acne scarring and reviving skin for a youthful, refreshed look."

Botox, dermal fillers and sexual wellness treatments join the medical spa's lineup of physician-administered aesthetic treatments, including PRP treatment for hair restoration and regrowth, PRP treatment for face and skin, vitamin drips, and IV therapy. For more information or to book an appointment, visit http://www.IVIVHealth.com.

ABOUT IVIV HEALTH AND AESTHETICS

Based in the heart of central New Jersey, IVIV Health and Aesthetics offers rejuvenation treatments in a safe and relaxing atmosphere. Under the care and guidance of a doctor and nurse practitioners, clients can receive treatments such as Juvderm, Botox, platelet-rich plasma (PRP) treatments for hair and skin rejuvenation, IV drip and vitamin therapy, and PRP-based sexual wellness treatments. For more information or to book an appointment, visit http://www.IVIVHealth.com.

SOURCE IVIV Health and Aesthetics

http://www.IVIVHealth.com

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Revolutionary Cosmetic Skin and Sexual Wellness Treatments Are Coming to Central New Jersey - PRNewswire

Is Stem Cell Therapy for Arthritis Safe and Effective?

People considering stem cell treatment for arthritis want to know Is it safe? and Is it effective?

Most stem cell therapy using adult stem cells is considered safe because the stem cells are collected from the patient, minimizing the risk of an unwanted reaction. The most common side effects are temporary swelling and pain.1

While most stem cell therapy for arthritis is considered safe, it does carry the same risks as any other medical procedure, such as a small risk of infection. Risk may be increased if:

See What Are Stem Cells?

Some research suggests stem cell therapy engaging in these kinds of practices may elevate the risk of tumors.2

As with most regenerative medicine treatments, research is ongoing, and FDA regulations are relatively new and subject to change.

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Whether or not stem cells therapy is effective in treating osteoarthritis is a controversial subject among medical professionals, and research in the area is ongoing.

See Osteoarthritis Treatment

How researchers think stem cell therapy worksResearchers theorize3 that when applied to an arthritic joint, stem cells might:

See Osteoarthritis Symptoms and Signs

It may be none, one, two, or all three processes at are work.

Proponents vs criticsLike many relatively new treatments, stem cell therapy has proponents and critics.

Critics emphasize that there have been no large-scale, prospective, double-blind research studiesthe kind of clinical studies that medical professionals consider the gold standardto support stem cell therapy for arthritis.

Factors that affect stem cell therapy researchAnother challenge associated with current stem cell research is that there is no standard stem cell therapy for arthritis treatment. So the stem cell therapy in one study is not necessarily the same as the stem cell therapy in another study.

Differences can include:

These differences are further complicated by more unknowns. For example, how many stem cells are needed for a particular treatment? And how do we determine if a patients own stem cells are competent enough to aid in healing?

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Many physicians combine the use of stem cells with platelet rich plasma, or PRP.

See Platelet-Rich Plasma (PRP) Therapy for Arthritis

PRP is derived from a sample of the patients blood. In the body, platelets secrete substances called growth factors and other proteins that regulate cell division, stimulate tissue regeneration, and promote healing. Like stem cell therapy, PRP therapy is sometimes used alone with the hopes of healing an arthritic joint.

See PRP Injection Preparation and Composition

Physicians who use PRP and stem cells together think that the PRP can help maximize the healing effects of stem cells.6,7 Research in this area is ongoing.

See Platelet-Rich Plasma Injection Procedure

Stem cell therapy can vary depending on the doctor performing it. People considering stem cell therapy for an arthritic knee or other joint are advised to ask their doctors questions, including:

Both doctors and patients can benefit from having a frank conversation and setting reasonable expectations.

See Arthritis Treatment Specialists

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Is Stem Cell Therapy for Arthritis Safe and Effective?

Stem Cell Treatments Flourish With Little Evidence That …

The companys website, loaded with impressive videos and testimonials from patients, is a major draw for aging gym rats searching the internet for relief from sore knees, shoulders, hips and backs.

Through the website, Dr. Centeno regularly criticizes other stem cell businesses, and has acted as an expert witness for injured patients suing his competitors.

The company did run afoul of the F.D.A. in 2008 over its use of cells cultured and multiplied in a lab to increase the stem cell count. After a protracted legal battle, Regenexx quit using that technique in the United States, but began offering it at a clinic on Grand Cayman.

This has always been about creating a less invasive orthopedic solution, what I call interventional orthopedics, Dr. Centeno said. He predicts a sea change in orthopedics similar to the revolution in cardiology, where much open-heart surgery was replaced by less invasive procedures.

While regulators may not consider them high risk, stem cell treatments involving bone marrow are not trivial. Collecting bone marrow involves forcefully puncturing the back of the hip bones in several spots, a painful process that requires local anesthesia. Then, pressure is applied to prevent bleeding, and the sites are bandaged to prevent infection.

Injecting the bone marrow or platelet extracts into the knee takes skill, even with X-rays to guide the needle. The injections can cause pain and irritation, and patients are usually sent home with leg braces that they will wear for a few weeks.

Sterile techniques are essential.

Whenever injections are administered to the joint, there is always a risk of introducing infection, said Dr. Kiran M. Perkins, who has investigated such illnesses at the Centers for Disease Control and Prevention. With stem cell treatments, she added, there are a lot of steps along the way where something could go wrong and you could have the introduction of microorganisms.

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Stem Cell Treatments Flourish With Little Evidence That ...

Stem-Cell Treatment for Blindness Moving Through Patient …

A new treatment for macular degeneration is close to the next stage of human testinga noteworthy event not just for the millions of patients it could help, but for its potential to become the first therapy based on embryonic stem cells.

This year, the Boston-area company Advanced Cell Technology plans to move its stem-cell treatment for two forms of vision loss into advanced human trials. The company has already reported that the treatment is safe (see Eye Study Is a Small but Crucial Advance for Stem-Cell Therapy), although a full report of the results from the early, safety-focused testing has yet to be published. The planned trials will test whether it is effective. The treatment will be tested both on patients with Stargardts disease (an inherited form of progressive vision loss that can affect children) and on those with age-related macular degeneration, the leading cause of vision loss among people 65 and older.

The treatment is based on retinal pigment epithelium (RPE) cells that have been grown from embryonic stem cells. A surgeon injects 150 microliters of RPE cellsroughly the amount of liquid in three raindropsunder a patients retina, which is temporarily detached for the procedure. RPE cells support the retinas photoreceptors, which are the cells that detect incoming light and pass the information on to the brain.

Although complete data from the trials of ACTs treatments have yet to be published, the company has reported impressive results with one patient, who recovered vision after being deemed legally blind. Now the company plans to publish the data from two clinical trials taking place in the U.S. and the E.U. in a peer-reviewed academic journal. Each of these early-stage trials includes 12 patients affected by either macular degeneration or Stargardts disease.

The more advanced trials will have dozens of participants, says ACTs head of clinical development, Eddy Anglade. If proved safe and effective, the cellular therapy could preserve the vision of millions affected by age-related macular degeneration. By 2020, as the population ages, nearly 200 million people worldwide will have the disease, estimate researchers. Currently, there are no treatments available for the most common form, dry age-related macular degeneration.

ACTs experimental treatment has its origins in a chance discovery that Irina Klimanskaya, the companys director of stem-cell biology, made while working with embryonic stem cells at Harvard University. These cells have the power to develop into any cell type, and in culture they often change on their own. A neuron here, a fat cell thereindividual cells in a dish tend to take random walks down various developmental paths. By supplying the cultures with fresh nutrients but otherwise leaving them to their own devices for several weeks, Klimanskaya discovered that the stem cells often developed into darkly pigmented cells that grew in a cobblestone-like pattern. She suspected that they were developing into RPE cells, and molecular tests backed her up.

Now that her discovery has advanced into an experimental treatment, Klimanskaya says she is excited by the hints that it may be able to preserve, and perhaps restore, sight. She recalls a voice mail she received during her second year at ACT: a person blinded by an inherited condition thanked her for her work, whether or not there was a treatment available for him. When you get a message like this, you feel like you are not doing it in vain, she says.

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Stem-Cell Treatment for Blindness Moving Through Patient ...

Stem Cell Therapy | Stem Cell Treatment | Charlotte, NC

As long as providers use autologous stem cells (definition: stem cells that come from the patient themselves) for regenerative therapy, the FDA accepts these procedures as complying with current standards of care. The equipment that we use to process your stem cells is FDA cleared for that use. All placental, amniotic, and umbilical cord blood/tissue products must have product-specific approval from the FDA, and as of now none of the commercially and currently available products have that approval except in one instance, and that is when umbilical cord blood stem cells are used specifically and only to treat hematological diseases like leukemia. No amniotic, placental, or umbilical cord blood or tissue products are FDA approved for regenerative medicine, and providers that currently use these products have only a limited time left before the FDA mandates that they must stop or face stiff sanctions. At Dynamic Health we have always used the patients own stem cells, and until more effective treatments are discovered and approved by the FDA we will continue to do so.

See the article here:
Stem Cell Therapy | Stem Cell Treatment | Charlotte, NC

Stem Cell Therapy New York | Stem Cell Treatment …

Stem cell therapy uses a patients own stem cells to treat minor to moderate conditions formally where surgery would be the primary option. Stem cell therapy is an alternative to surgery through an office based procedure. Through advanced technology stem cell therapy is now a quick and easy alternative to surgery.

What are stem cells?

Stem cells have the remarkable potential to develop into many different cell types in the body during early life and growth. In addition, in many tissues they serve as of internal repair system, dividing essentially without limit to replenish other cells. When a stem cell divides, each new cell has the potential either to remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell.

Regenerative Growth Factors Stem cells have high natural growth factors that promote healing. These growth factors also naturally decrease inflammation.

Patients stem cells are obtained from their pelvic (hip) bone. The practitioner then concentrates the stem cells over 10 fold in a special centrifuge.

This office based procedure takes 30 minutes. Patients can leave at the conclusion of the procedure.

A local anesthetic is used. This results in minimal discomfort.

There have been numerous clinical studies that show significant and lasting reduction in pain for a variety of injuries. Common injuries include early to advanced arthritis, knee and shoulder pain.

Multiple clinical studies have shown that when effective, the results last many years.

Yes see the article section

85% of patients experience a benefited result from treatment

Most patients feel no improvement for at least 3 weeks and possible 6-8 weeks. Once you feel improvement, you will notice continued improvement expanding over 6 months.

Link:
Stem Cell Therapy New York | Stem Cell Treatment ...

Stem Cell Treatment for Ataxia – Stem Cell Treatment Now

How does ataxia affect the patient? The disease is characterized by progressively disabling clinical manifestations. Patients show symptoms of gait instability or dysarthria and may begin to fall without warning. Gradually they present progressive limitations in their activities, lose the ability to walk, become bedridden and fully dependent. Other clinical manifestations include astasia, impaired fine motor skillsand intention tremor (cerebellar tremor). The cerebellar syndrome is often associated with other neurological signs such as pyramidal or extrapyramidal signs, ophthalmoplegia, and cognitive impairment.

How to diagnose ataxia? Most people dont know what ataxia is and may overlook the early symptoms. Anyone with progressive gait disorder or imbalance should be evaluated by a neurologist. MRI is recommended in all cases. If a treatable cause is not discovered, a gene test should be done. In about 60 percent of the cases, the gene test will determine the type of ataxia.

What is the efficacy of conventionaltreatments? Until now, conventional treatments are generally used to alleviate the symptoms, not the disease itself. The movement disorders can be managed using pharmacological, physical and occupational therapies to minimize the damage and to promote the mobility as long as possible but overall current treatment remains retardant.

What are the difference between autosomal dominant and autosomal recessive ataxias? Autosomal dominant and autosomal recessive ataxias are hereditary ataxias, and spinocerebellar ataxia (SCA) and Friedreich's ataxia (FRDA) are the most common forms of hereditary ataxia. Autosomal dominant genes express themselves when present. Autosomal recessive genes will only express themselves when in the homozygous state -- i.e., both genes in the gene pair are the recessive gene form. Thus, recessive genes can be "carried" by those whose phenotype does not exhibit the gene characteristic, while dominant genes cannot be "carried". Therefore, generally autosomal dominant ataxias are easier to express andat a higher morbiditythan autosomal recessive ataxias.

What is the role of Purkinje cells, where can we find those cells ? Purkinjecellsare a type of neuron found in the cerebellar cortex, at the base of the brain.They are among the largest neurons and are responsible for most of the electrochemical signaling in the cerebellum. ThePurkinjecellsand the cerebellum are essential to the body's motor function. Disorders involving thePurkinjecellsusually negatively affect the patient's movements.

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Stem Cell Treatment for Ataxia - Stem Cell Treatment Now

Knees – Stem Cell Therapy For Pain | Stem Cell Seminars …

Surgical options for those suffering from painful knee injuries can range from knee surgery to complete knee replacement. Recent studies have shown that most surgeries dont have a 100% success rate and there is chance of the pain and discomfort resurfacing. Knee replacement surgery can be extremely traumatic and it has the risk of toxic artificial particles entering the bloodstream. Apart from this, months of a painful recovery phase is required to regain the strength and mobility following the surgeries. Most surgeries also boost the degeneration process, which can lead to osteoarthritis.

Knee replacement surgery carries many serious risks and complications, but is often considered as the most-accepted treatment method for those dealing with significant arthritis or injury in knee joints.

Surgery should always be the last option, however, it is often perceived to be the only treatment alternative by most patients. At Stem Cell Therapy for Pain, we have assisted several patients with severe arthritis problems in avoiding knee replacement surgery through stem cell treatment for knees, which enabled them to carry out the things they love without being burdened by pain. We have seen exciting results on tricky knee injury cases, including complete ligament and muscle tears. Our expert physicians ensure that patients experience little to no downtime after the treatment, and can bounce back to their daily routine immediately.

The most common form of arthritis is Osteoarthritis. It is also known as wear-and-tear arthritis, since the cartilage thins and eventually breaks down. The cartilage cushions the joints where two bones meet, so any degeneration can seriously impact our body.

Stem cell treatments for knees offered by Stem Cell Therapy for Pain are designed to target the knee joints to assist with the formation of new cartilage cells. The aim of each stem cell injection for knee pain is to re-inject the amniotic or adult stem cells into the knee joint to boost cartilage or chondrocytes cell creation. Stem cells have a natural anti-inflammatory chemical that helps to alleviate osteoarthritis pain and inflammation in the knee joint.

If you have chronic knee pain due to a past injury or arthritis, an injury to the knee cartilage, meniscus, ligaments, you might be an ideal candidate for stem cell therapy for knees.

To learn more about services like stem cell therapy for osteoarthritis, get in touch with us today. Lets work on restoring your life, one treatment at a time!

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Knees - Stem Cell Therapy For Pain | Stem Cell Seminars ...

Second patient free of HIV after stem-cell therapy

A person with HIV seems to be free of the virus after receiving a stem-cell transplant that replaced their white blood cells with HIV-resistant versions. The patient is only the second person ever reported to have been cleared of the virus using this method. But researchers warn that it is too early to say that they have been cured.

The patient whose identity hasnt been disclosed was able to stop taking antiretroviral drugs, with no sign of the virus returning 18 months later. The stem-cell technique was first used a decade ago for Timothy Ray Brown, known as the Berlin patient, who is still free of the virus.

So far, the latest patient to receive the treatment is showing a response similar to Browns, says Andrew Freedman, a clinical infectious-disease physician at Cardiff University in the UK who was not involved in the study. Theres good reason to hope that it will have the same result, he says.

Like Brown, the latest patient also had a form of blood cancer that wasnt responding to chemotherapy. They required a bone-marrow transplant, in which their blood cells would be destroyed and replenished with stem cells transplanted from a healthy donor.

But rather than choosing just any suitable donor, the team led by Ravindra Gupta, an infectious-disease physician at the University of Cambridge, UK picked a donor who had two copies of a mutation in the CCR5 gene that gives people resistance to HIV infection. This gene codes for a receptor which sits on the surface of white blood cells involved in the bodys immune response. Normally, the HIV binds to these receptors and attacks the cells, but a deletion in the CCR5 gene stops the receptors from functioning properly. About 1% of people of European descent have two copies of this mutation and are resistant to HIV infection.

Guptas team describes the results in a paper due to be published in Nature on 5 March. The researchers report that the transplant successfully replaced the patients white blood cells with the HIV-resistant variant. Cells circulating in the patients blood stopped expressing the CCR5 receptor, and in the lab, the researchers were unable to re-infect these cells with the patients version of HIV.

The team found that the virus completely disappeared from the patients blood after the transplant. After 16 months, the patient stopped taking antiretroviral drugs, the standard treatment for HIV. In the latest follow-up, 18 months after stopping medication, there was still no sign of the virus.

Gupta says that its not yet possible to say whether the patient has been cured. This can only be demonstrated if the patients blood remains HIV free for longer, he says.

But the study does suggest that Browns successful treatment ten years ago wasnt just a one-off. Gupta says that the latest patient received a less aggressive treatment than Brown to prepare for the transplant. The new patient was given a regimen consisting of chemotherapy alongside a drug that targets cancerous cells, while Brown received radiotherapy across his entire body in addition to a chemotherapy drug.

This suggests that, to be successful, stem-cell transplants in HIV patients would not necessarily need to be accompanied by aggressive treatments that might have particularly severe side effects, says Gupta. The radiation really does knock the bone marrow and make you very sick.

Graham Cooke, a clinical researcher at Imperial College London, points out that this kind of treatment wouldnt be suitable for most people with HIV who dont have cancer and so dont need a bone-marrow transplant, which is a serious procedure that can sometimes have fatal complications. If youre well, the risk of having a bone-marrow transplant is far greater than the risk of staying on tablets every day, he says. Most people with HIV respond well to daily antiretroviral treatment.

But Cooke adds that for those who need a transplant to treat leukaemia or other diseases, it seems reasonable to try and find a donor with the CCR5 mutation, which wouldnt add any risk to the procedure.

Gero Htter, who led Browns treatment and is now medical director of the stem-cell company Cellex in Dresden, Germany, agrees that this kind of treatment could only ever be used for a small group of patients. But he hopes that the paper will stimulate a renewed interest in gene therapies that target CCR5, which could be applied to a much broader group. The real breakthrough, we are still waiting for, he says.

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Second patient free of HIV after stem-cell therapy