Keeping the Kraken asleep

IMAGE:CDK6 is needed for leukemic stem cell activation (left). When CDK6 is absent, the LSC remains in a quiescent state and leukemia formation is prohibited (right). view more

Credit: Angelika Berger / Vetmeduni Vienna

Despite enormous progress in cancer therapy, many patients still relapse because their treatment addresses the symptoms of the disease rather than the cause, the so-called stem cells. Work in the group of Veronika Sexl at the University of Veterinary Medicine, Vienna has given a tantalizing clue to a solution. In the current issue of Blood, the scientists report that the cell-cycle kinase CDK6 is required for activation of the stem cells responsible for causing leukemia.

Hematopoietic stem cells (HSCs) are normally inactive, i.e. quiescent. When new blood cells are needed, for example to replace blood that has been lost, HSCs start to multiply and develop into mature blood cells. If the process is initiated at an inappropriate time, hematopoietic diseases such as leukemia may result and leukemic stem cells may develop. These represent a major challenge to leukemia therapy: they are quiescent and thus protected from elimination by the immune system and from treatment such as chemotherapy. Leukemic stem cells frequently cause relapse in cancer patients, often years or even decades after an apparently successful treatment.

Working with stem cells isolated from mice, Ruth Scheicher and colleagues at the University of Veterinary Medicine, Vienna have investigated possible differences between leukemic stem cells and the healthy stem cells in the body. They looked in particular at the function of the CDK6 protein, which is known to be involved in controlling the cell cycle. Surprisingly, CDK6 was also found to regulate the activation of hematopoietic and leukemic stem cells, which it does by inhibiting the transcription factor Egr1. Upon loss of CDK6, Egr1 becomes active and prevents stem cells from dividing. In a further twist to the tale, the mechanism operates only when hematopoietic stem cells are stressed, e.g. in leukemia, and not in the normal physiological situation.

Scheicher is quick to note the significance of her finding. "CDK6 is absolutely necessary for leukemic stem cells to induce disease but plays no part in normal hematopoiesis. We thus have a novel opportunity to target leukemia at its origin. Inhibiting CDK6 should attack leukemic stem cells while leaving healthy HSCs unaffected".

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Service: The article 'CDK6 as a key regulator of hematopoietic and leukemic stem cell activation' by Scheicher R, Hoelbl-Kovacic A, Bellutti F, Tigan AS, Prchal-Murphy M, Heller G, Schneckenleithner C, Salazar-Roa M, Zchbauer-Mller S, Zuber J, Malumbres M, Kollmann K and Sexl V. was published in the journal Blood. http://www.bloodjournal.org/content/125/1/90.long?sso-checked=true

About the University of Veterinary Medicine, Vienna

The University of Veterinary Medicine, Vienna in Austria is one of the leading academic and research institutions in the field of Veterinary Sciences in Europe. About 1,300 employees and 2,300 students work on the campus in the north of Vienna which also houses five university clinics and various research sites. Outside of Vienna the university operates Teaching and Research Farms. http://www.vetmeduni.ac.at

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Keeping the Kraken asleep

Sask. man off to Malaysia for stem cell treatment

A 28-year-old North Battleford man plans to head to Malaysia next month for a stem cell treatment he hopes will cure his debilitating liver disease.

Kyle Allan is able to pay for the trip thanks to the success of a gofundme.com campaign, which rocketed past its $25,000 goal in just one week.

As of Monday, he'd raised $61,425.

"It was mind blowing," Allan said.

His girlfriend, Valerie Blais, who set up the campaign, had no idea it would do so well and had even expected to do additional fundraising.

"It's completely overwhelming. It shows so much love and support from people in our lives and the community," she said.

When Allan was about three years old, he repeatedly contracted pneumonia and other infections, and was diagnosed with Common Variable Immunodefiency, for which no cure has been identified.

He previously received an IV treatment once a month, which has now increased to once per day. Growing up, he was often tired and didn't have as much energy as other children, but as he got into his teens he improved.

"I was pretty much a normal kid," he said.

He became a welder and worked in Fort McMurray, then got his journeyman qualifications at SIAST. He was about to head back north when, on Aug. 18, 2008, he couldn't get out of bed.

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Sask. man off to Malaysia for stem cell treatment

Using stem cells to grow new hair

In a new study from Sanford-Burnham Medical Research Institute (Sanford-Burnham), researchers have used human pluripotent stem cells to generate new hair. The study represents the first step toward the development of a cell-based treatment for people with hair loss. In the United States alone, more than 40 million men and 21 million women are affected by hair loss. The research was published online in PLOS One yesterday.

"We have developed a method using human pluripotent stem cells to create new cells capable of initiating human hair growth. The method is a marked improvement over current methods that rely on transplanting existing hair follicles from one part of the head to another," said Alexey Terskikh, Ph.D., associate professor in the Development, Aging, and Regeneration Program at Sanford-Burnham. "Our stem cell method provides an unlimited source of cells from the patient for transplantation and isn't limited by the availability of existing hair follicles."

The research team developed a protocol that coaxed human pluripotent stem cells to become dermal papilla cells. They are a unique population of cells that regulate hair-follicle formation and growth cycle. Human dermal papilla cells on their own are not suitable for hair transplants because they cannot be obtained in necessary amounts and rapidly lose their ability to induce hair-follicle formation in culture.

"In adults, dermal papilla cells cannot be readily amplified outside of the body and they quickly lose their hair-inducing properties," said Terskikh. "We developed a protocol to drive human pluripotent stem cells to differentiate into dermal papilla cells and confirmed their ability to induce hair growth when transplanted into mice."

"Our next step is to transplant human dermal papilla cells derived from human pluripotent stem cells back into human subjects," said Terskikh. "We are currently seeking partnerships to implement this final step."

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The above story is based on materials provided by Sanford-Burnham Medical Research Institute. Note: Materials may be edited for content and length.

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Using stem cells to grow new hair

Stamina doctor Davide Vannoni in 22-month plea bargain

Psychologist on trial for criminal association, fraud

(ANSA) - Turin, January 27 - A psychologist who developed the now-banned Stamina stem-cell treatment for terminal nerve disease patients entered a formal request for a plea bargain in a trial against him during a preliminary hearing on Tuesday. Davide Vannoni requested a plea deal of one year and 10 months. In Italy, custodial sentences of under three years are usually suspended. Prosecutor Raffaele Guariniello backed the request, saying the withdrawal by Vannoni of an appeal to the Lazio administrative court against a provision rejecting the controversial therapy eliminated all doubt concerning the possible reiteration of the crime. Vannoni is on trial with 13 others on charges including aggravated criminal association with intent to commit fraud in connection with the therapy, which he used on terminally ill degenerative nerve disease patients. The health ministry last November decreed the end of experimentation with the controversial Stamina treatment, which supporters say could help cure degenerative nerve diseases but which experts say lacks a scientific basis. The credibility of the Stamina treatment, which involves extracting bone-marrow stem cells from a patient, supposedly turning them into neurons by exposing them to retinoic acid for two hours, and injecting them back into the patient, has long been suspect. The health ministry in late 2013 ruled that the Stamina Foundation would no longer be allowed to test the treatment on humans, and it was stripped of its non-profit status. A panel of government-appointed experts said last year it found the therapy seriously lacking in both premise and practice. Their report cited "serious imperfections and omissions in the Stamina protocol, including conceptual errors and an apparent ignorance of stem-cell biology". Vannoni told Nature magazine in a 2013 interview that he developed the therapy after receiving what he said was stem-cell treatment for a virus-induced facial paralysis in Russia in 2004.

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Stamina doctor Davide Vannoni in 22-month plea bargain

Heard about a public stem cell bank in Chennai?

CHENNAI: There is a public stem cell bank in Chennai where people can donate stem cell stems, but most clinicians and people are unaware of it.

In her presentation at the 58th All India Congress of Obstetrics and Gynaecology, Dr Saranya Narayan, medical director and co-founder of Jeevan Stem Cell Bank, said many clinicians were not aware of the existence of the public stem cell bank in the city that could store stem cells for free of cost and help patients with hematological illnesses like thalassemia and blood cancer.

"The only operational public stem cell bank is in Chennai. We function with the grant from the Tamil Nadu government and help from the World Bank. But many clinicians are not aware of this public bank," the doctor said.

She explained that stem cell is separated from the umbilical cord blood which is collected during the birth of a baby and is stored.

While awareness is important, the doctor had also observed that many clinicians feel that counselling families for donating cord blood is eating into their consultation time.

"We have sent our staff members for counselling families in some hospitals. But with the pamphlets and awareness material, it is easy to convince families to donate. We also found that some doctors say they do not have an infrastructure to collect cord blood. We give them the kit to collect the cord blood," she explained.

The lack of awareness among people on the benefits of stem cells leaves about 90 percent of cord blood ending up as a medical waste, the doctor said.

"There is a 60 to 80 percent success rate in stem cell transplant treatment for hematological illnesses. So a donor's stem cell is used on someone else with the illness. But if the donor or donor's family has an illness and needs it, they can approach us. We will either give the donor's own stem cells or find a suitable match and provide them at a concessional rate," Dr Saranya said.

While the level of donation of umbilical cord blood is low, the doctor noted that about 40 percent of cord blood donations are rejected for various reasons, including volume below 60ml, leaks from blood bag, large clots in blood bag and delay in transportation of the cord blood for preservation.

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Heard about a public stem cell bank in Chennai?

Woman left housebound and suicidal by rare illness that has made her face and body "balloon"

A WOMAN whose face and body swell up and balloon because of a rare illness hopes new stem cell treatment available in the US could rid her of the condition.

Christie Newport, 33, is virtually housebound after bullies taunted her with the nickname "rubber dingy lips", leaving her contemplating suicide.

She now hopes to raise 12,000 to pay for new specialist treatment in Califirnia.

Christie's lips, stomach, arms and legs have gradually bloated due to Melkersson Rosenthal syndrome. But a range of procedures and medicines in the UK, including plastic surgery on her lips, all failed.

The US treatment costs a minimum of 12,000, but its arrival has left Christie determined to beat the condition. She said: "I have one of the worst cases there is and I just don't feel like I have a life any more.

"It's like it has been stolen by my illness. I'm prepared to do anything to get the treatment because I can't live like this. If I could avoid living like this I would do it tomorrow."

Christie, from Ashton-on-Ribble, Lancs, was diagnosed with the condition at the age of 18, despite dealing with symptoms since childhood.

Her health has worsened to the point her airways swell and she has problems with her lungs and extreme pain and now has type two steroid induced diabetes and a paralysed right diaphragm. She is also infertile.

She said: "It's spread through my body now and I am lucky if I can get a couple of hours out of bed in the day. It has made me realise how limited my life has become."

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Woman left housebound and suicidal by rare illness that has made her face and body "balloon"

Heard about a free stem cell bank in Chennai?

CHENNAI: There is a public stem cell bank in Chennai where people can donate stem cell stems, but most clinicians and people are unaware of it.

In her presentation at the 58th All India Congress of Obstetrics and Gynaecology, Dr Saranya Narayan, medical director and co-founder of Jeevan Stem Cell Bank, said many clinicians were not aware of the existence of the public stem cell bank in the city that could store stem cells for free of cost and help patients with hematological illnesses like thalassemia and blood cancer.

"The only operational public stem cell bank is in Chennai. We function with the grant from the Tamil Nadu government and help from the World Bank. But many clinicians are not aware of this public bank," the doctor said.

She explained that stem cell is separated from the umbilical cord blood which is collected during the birth of a baby and is stored.

While awareness is important, the doctor had also observed that many clinicians feel that counselling families for donating cord blood is eating into their consultation time.

"We have sent our staff members for counselling families in some hospitals. But with the pamphlets and awareness material, it is easy to convince families to donate. We also found that some doctors say they do not have an infrastructure to collect cord blood. We give them the kit to collect the cord blood," she explained.

The lack of awareness among people on the benefits of stem cells leaves about 90 percent of cord blood ending up as a medical waste, the doctor said.

"There is a 60 to 80 percent success rate in stem cell transplant treatment for hematological illnesses. So a donor's stem cell is used on someone else with the illness. But if the donor or donor's family has an illness and needs it, they can approach us. We will either give the donor's own stem cells or find a suitable match and provide them at a concessional rate," Dr Saranya said.

While the level of donation of umbilical cord blood is low, the doctor noted that about 40 percent of cord blood donations are rejected for various reasons, including volume below 60ml, leaks from blood bag, large clots in blood bag and delay in transportation of the cord blood for preservation.

Stay updated on the go with Times of India News App. Click here to download it for your device.

See more here:
Heard about a free stem cell bank in Chennai?

Stamina doc Davide Vannoni offers plea bargain

Psychologist on trial for criminal association, fraud

(ANSA) - Turin, January 23 - A psychologist who developed the now-banned Stamina stem-cell treatment for terminal nerve disease patients has tried to plea bargain his way out of trial, sources said Friday. Davide Vannoni and 13 co-defendants are on trial on charges including aggravated criminal association with intent to commit fraud in connection with the therapy, which he used on terminally ill degenerative nerve disease patients. Vannoni offered to withdraw his suit against the health ministry and to shut down his Stamina Foundation in Italy in exchange for a prison sentence of one year and ten months. In Italy, custodial sentences of under three years are usually suspended. The health ministry last November decreed the end of experimentation with the controversial Stamina treatment, which supporters say could help cure degenerative nerve diseases but which experts say lacks a scientific basis. The credibility of the Stamina treatment, which involves extracting bone-marrow stem cells from a patient, supposedly turning them into neurons by exposing them to retinoic acid for two hours, and injecting them back into the patient, has long been suspect. The health ministry in late 2013 ruled that the Stamina Foundation would no longer be allowed to test the treatment on humans, and it was stripped of its non-profit status. A panel of government-appointed experts said last year it found the therapy seriously lacking in both premise and practice. Their report cited "serious imperfections and omissions in the Stamina protocol, including conceptual errors and an apparent ignorance of stem-cell biology". Vannoni told Nature magazine in a 2013 interview that he developed the therapy after receiving what he said was stem-cell treatment for a virus-induced facial paralysis in Russia in 2004. The court will decide next week whether to accept his plea bargain, sources said.

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Stamina doc Davide Vannoni offers plea bargain

Broeska breaks silence, responds to coverage of MS treatment operation

Winnipeg Free Press - ONLINE EDITION

By: Mary Agnes Welch

Posted: 10:43 AM | Comments: | Last Modified: 12:19 PM | Updates

After days of silence, the Winnipeg medical researcher who charged MS patients thousands for overseas stem cell treatment says hes been "unfairly accused and victimized by inaccurate media reporting" and looks forward to clearing his name.

In a statement sent to local media Friday morning, Doug Broeska, owner of Regenetek Research says media reports that he falsified his credentials, overstated the effects of the stem cell clinical trial, failed to follow up with patients and was recently asked to step down as the studys researcher are false.

He said he stands by his role in the "case-based study" where his job was to track patients for follow-up. He said he has not breached ethical standards and did not give medical advice to patients.

He also enclosed a notarized copy of his PhD certificate from Brightland University, which is not accredited in Canada, the United States or the United Kingdom. Brightlands website includes no contact or location information, spells Brightland incorrectly and is linked to a well-known degree mill operator

He also enclosed a notarized copy of his PhD certificate from Brightland University, which is not accredited in Canada, the United States or the United Kingdom. Brightlands website includes no contact or location information, spells "Brightland" incorrectly and is linked to a well-known degree mill operator.

Broeskas degree certificate, notarized by Liya Akalu, a notary public in Washington, D.C., includes a stamp saying the oath was sworn before her Dec. 15, 2015, a date that has not yet occurred.

Broeska originally claimed to have a BSc and a PhD from the University of Manitoba. Neither is true.

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Broeska breaks silence, responds to coverage of MS treatment operation

The Miami Stem Cell Treatment Center Announces Adult Stem Cell Public Seminars in Naples, Florida

Naples, Florida (PRWEB) January 23, 2015

The Miami Stem Cell Treatment Center announces a series of free public seminars on the use of adult stem cells for various degenerative and inflammatory conditions. They will be provided by Dr. Thomas A. Gionis, Surgeon-in-Chief and Dr. Nia Smyrniotis, Medical Director and Surgeon.

The first seminar will be held on Sunday, January 25, 2015, at 11:00am, 1:00pm and 3:00pm at the Hilton Naples, 5111 Tamiami Trail North, Naples, FL 34103. Please RSVP at (561) 331-2999.

The Miami Stem Cell Treatment Center (Miami; Boca Raton; Orlando), along with sister affiliates, the Irvine Stem Cell Treatment Center (Irvine; Westlake Villages, Ca.) and the Manhattan Regenerative Medicine Medical Group (Manhattan, New York), abide by approved investigational protocols using adult adipose derived stem cells (ADSCs) which can be deployed to improve patients quality of life for a number of chronic, degenerative and inflammatory conditions and diseases. ADSCs are taken from the patients own adipose (fat) tissue (found within a cellular mixture called stromal vascular fraction (SVF)). ADSCs are exceptionally abundant in adipose tissue. The adipose tissue is obtained from the patient during a 15 minute mini-liposuction performed under local anesthesia in the doctors office. SVF is a protein-rich solution containing mononuclear cell lines (predominantly adult autologous mesenchymal stem cells), macrophage cells, endothelial cells, red blood cells, and important Growth Factors that facilitate the stem cell process and promote their activity.

ADSCs are the bodys natural healing cells - they are recruited by chemical signals emitted by damaged tissues to repair and regenerate the bodys injured cells. The Miami Stem Cell Treatment Center only uses Adult Autologous Stem Cells from a persons own fat No embryonic stem cells are used; and No bone marrow stem cells are used. Current areas of study include: Emphysema, COPD, Asthma, Heart Failure, Heart Attack, Parkinsons Disease, Stroke, Traumatic Brain Injury, Lou Gehrigs Disease, Multiple Sclerosis, Lupus, Rheumatoid Arthritis, Crohns Disease, Muscular Dystrophy, Inflammatory Myopathies, and degenerative orthopedic joint conditions (Knee, Shoulder, Hip, Spine).

For more information, or if someone thinks they may be a candidate for one of the adult stem cell protocols offered by the Miami Stem Cell Treatment Center, they may contact Dr. Gionis or Dr. Smyrniotis directly at (561) 331-2999, or see a complete list of the Centers study areas at: http://www.MiamiStemCellsUSA.com.

About the Miami Stem Cell Treatment Center: The Miami Stem Cell Treatment Center, along with sister affiliates, the Irvine Stem Cell Treatment Center and the Manhattan Regenerative Medicine Medical Group, is an affiliate of the California Stem Cell Treatment Center / Cell Surgical Network (CSN); we are located in Boca Raton, Orlando, Miami and The Villages (opening soon), Florida. We provide care for people suffering from diseases that may be alleviated by access to adult stem cell based regenerative treatment. We utilize a fat transfer surgical technology to isolate and implant the patients own stem cells from a small quantity of fat harvested by a mini-liposuction on the same day. The investigational protocols utilized by the Miami Stem Cell Treatment Center have been reviewed and approved by an IRB (Institutional Review Board) which is registered with the U.S. Department of Health, Office of Human Research Protection (OHRP); and our studies are registered with Clinicaltrials.gov, a service of the U.S. National Institutes of Health (NIH).

For more information, visit our websites: http://www.MiamiStemCellsUSA.com, http://www.IrvineStemCellsUSA.com , or http://www.NYStemCellsUSA.com.

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The Miami Stem Cell Treatment Center Announces Adult Stem Cell Public Seminars in Naples, Florida