ABOUT THE COURSE

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This a hands-on practical course on Induced pluripotent stem cells (iPSCs) differentiation into hepatocytes.iPSCs provide an inexhaustible source of cells, which can bedifferentiated into any lineage. iPSCs are generated from normal and disease adult cells (such asblood cells or skin cells) via reprogramming using a defined set of transcription factors (Oct4, Sox2, c-Myc, Kif4).Hepatocytes are liver cells, which make up 70-85 % of the liver mass. These cells are involved inprocesses such as protein synthesis, carbohydrate metabolism and detoxification, which areassociated with many diseases. iPSC derived hepatocytes can be used for basic liver research such asunderstanding liver development and cell biology, as well as for disease modelling and toxicity screening.

In this Induced pluripotent stem cells (iPSCs) differentiation into hepatocytes courses:

1. Participants will be introduced to the background, maintenance and applications of iPSC technology. 2. During the hands-on practical, participants will learn how to derive hepatocytes from human iPSCs. 3. Participants will take part in interactive tutorials, Q&A clinics, and panel discussions, with leading scientistswho will answer any questions you may have about your individual projects, to help you to avoid the commonpitfalls of using iPSCs in your experiments.

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OTHER INFORMATION

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LEARNINGOBJECTIVESOF THIS IPSCs DIFFERENTIATION INTO HEPATOCYTE COURSE:

1. To understand the history, manipulation and use of iPSCs 2. To gain knowledge of hepatocyte structure and function 3. Learn how to differentiate human iPSCs into hepatocytes 4. To gain knowledge about the use of differentiated hepatocytes in both basic research anddisease modelling

courses@cambioscience.com

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PROGRAMME

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INSTRUCTORS

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Professor David Hay

MRC Centre for Regenerative Medicine, University of Edinburgh

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David Hay is Professor of Tissue Engineering at the University of Edinburgh. David has worked in the field of stem cell biology and differentiation for over fifteen years. David andhis team have highlighted the important role that pluripotent stem cells have to play inmodelling human liver biology in a dish and supporting failing liver function in vivo. Theimpact of this work has led to a number of peer reviewed publications, regular appearancesat high profile conferences and three start-up companies.

Dr Rute Tomaz

Wellcome-MRC Cambridge,Stem Cell Institute and the Department of Surgery

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Dr Rute Tomaz is a postdoc research associate at Dr Ludovic Valliers lab, part of theWellcome-MRC Cambridge Stem Cell Institute and the Department of Surgery. Since joiningthe lab in 2016, her research has focused on the developing novel methods fordifferentiation and maturation of hepatocytes from human pluripotent stem cells forsubsequent applications such as disease modelling and drug screening. Prior to joining thislab, Rute did her PhD at Imperial College London where she studied gene regulationmechanisms in early cell fate choices using mouse embryonic stem cells as a model.

Dr Daniel Ortmann

Wellcome-MRC Cambridge, Stem Cell Institute and the Department of Surgery

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Dr Florian Merkle

Principal Investigator, Metabolic Research Laboratories, University of Cambridge

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The Merkle laboratory studies the molecular and cellular basis of human diseases using a combination of human cellular models and animal models. They have a particular interest in obesity, which leads to millions of premature deaths each year, lacks broadly effective treatments, and is associated with the aberrant function of specific neuron types in the hypothalamus. The lab developed methods to differentiate human pluripotent stem cells (hPSCs) into functional hypothalamic cell types in culture, enabling us to study their function in health and obesity using a range of cutting-edge techniques including genome engineering, single-cell transcriptomics, quantitative peptidomics, high content imaging, calcium imaging, and xenotransplantation. Research in the Merkle laboratory revolves around three areas: 1) Basic biology of human hypothalamic neurons 2) Genetic and environmental contributions to obesity 3) Translation and in vivo models Dr. Merkle is affiliated to the Cambridge Stem Cell Institute,collaborates with the Wellcome Sanger Institute and EBI in the context of his single-cell RNAseq work, and was recently awarded the prestigious Sir Henry Dale fellowship, during which he will use gene editing to explore how obesity-associated mutations alter cellular phenotypes.

Dr Annika Asplund

Senior Scientist, Takara Bio

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Annika Asplund is a Senior Research Scientist at Takara Bio Europe, AB in Sweden. She completed her PhD in molecular medicine within the field of cardiovascular prevention at Gothenburg University, Sweden. In 2012 she joined Takara Bio, focusing on hepatocyte differentiation and maturation from human pluripotent stem cells. Annika has extensive experience in protocol development for hepatocyte differentiation and characterization as well as dissociation and cryopreservation and contributed to the development of several innovative solutions offered by Takara Bio. She is committed to improving Takara Bios hepatocyte solutions and to helping customers succeeding with their hepatocyte related experiments.

Dr Johannes Elvin

Senior Production and Service Specialist, Takara Bio

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Johannes Elvin is a Senior Production and Service Specialist and joined Takara Bio Europe AB in 2018. He did his PhD at Gothenburg University in renal medicine and transitioned into the field of diabetes and obesity during his postdoc. He has been working extensively with in vitro cell culture, lentiviral transduction and protein expression modification. He is now handling and running service projects aimed at reprogramming somatic cells into pluripotent stem cells.

Dr Christian Andersson

Product Development Manager, Takara Bio

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Christian Andersson is a senior scientist and Product Development Manager at Takara Bio Europe, AB. He has extensive experience of culturing and differentiate stem cells into preferred cell types, where his field of expertise are Beta Cells. He has worked in project team to commercialize, market and promote Takara Bios stem cell portfolio all over the world. He is part of the companys stem cell strategy and marketing team, defining stem cell projects, marketing approaches, and business development opportunities.

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REGISTRATION DETAILS

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17 SPACES AVAILABLE

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With this course you get our exclusive CamBioScience membership for free and receive an automatic 10% discount on your registration fee.

* These fees include your 10% discount.

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To register, please click below and fill in the registration form. You will then receive an email confirmation in which you will be able to choose a payment method (pay by card online, or receive an invoice to process payment by bank transfer).

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PARTNERS

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IPSCjun19 Induced Pluripotent Stem Cells: differentiation ...