Stem Cell Clinic

Allogeneic Stem Cell Transplantation | Leukemia and …

By Stem Cell Treatment

Allogeneic stem cell transplantation involves transferring the stem cells from a healthy person (the donor) to your body after high-intensity chemotherapy or radiation.

Allogeneic stem cell transplantation is used to cure some patients who:

Allogeneic stem cell transplantation can be a high-risk procedure. The high-conditioning regimens are meant to severely or completely impair your ability to make stem cells and you will likely experience side effects during the days you receive high-dose conditioning radiation or chemotherapy. The goals of high-conditioning therapy are to:

The immune system and the blood system are closely linked and can't be separated from each other. Because of this, allogeneic transplantation means that not only the donor's blood system but also his or her immune system is transferred. As a result, these adverse effects are possible:

The immune reaction, or GVHD, is treated by administering drugs to the patient after the transplant that reduce the ability of the donated immune cells to attack and injure the patient's tissues.SeeGraft Versus Host Disease.

Allogeneic stem cell transplants for patients who are older or have overall poor health are relatively uncommon. This is because the pre-transplant conditioning therapy is generally not well tolerated by such patients, especially those with poorly functioning internal organs. However,reduced intensity allogeneic stem cell transplantsmay be an appropriate treatment for some older or sicker patients.

One goal of allogeneic stem cell transplant is to cause the T lymphocytes in the donor's blood or marrow to take hold (engraft) and grow in the patient's marrow. Sometimes the T lymphocytes attack the cancer cells. When this happens, it's called graft versus tumor (GVT) effect (also called graft versus cancer effect). The attack makes it less likely that the disease will return. This effect is more common in myeloid leukemias than it is in other blood cancers.

Unfortunately, T lymphocytes are the same cells that causegraft versus host disease(GVHD). Because of this serious and sometimes life-threatening side effect, doctors in certain cases want to decrease the number of T lymphocytes to be infused with the stem cells. This procedure, called T-lymphocyte depletion, is currently being studied by researchers. The technique involves treating the stem cells collected for transplant with agents that reduce the number of T lymphocytes.

The aim of T-lymphocyte depletion is to lessen GVHD's incidence and severity. However, it can also cause increased rates of graft rejection, a decreased GVT effect and a slower immune recovery. Doctors must be careful about the number of T lymphocytes removed when using this technique.

Stem cell selection is another technique being studied in clinical trials that can reduce the number of T lymphocytes that a patient receives. Because of specific features on the outer coat of stem cells, doctors can selectively remove stem cells from a cell mixture. This technique produces a large number of stem cells and fewer other cells, including T lymphocytes.

If you're considering allogeneic stem cell transplantation, you'll need a bone marrow donor. First, you and your siblings, if any, will have your blood or a scraping from your inside cheek tested to determine tissue type. A sibling has the potential to match you most closely because you both received your genes from the same parents.

A lab technician examines the surface of the sample tissue cells to identify the proteins that give everyone his or her own unique tissue type, called human leukocyte antigens (HLAs). If the HLA on the donor cells are identical (from identical twins, for example) or similar (such as those from siblings), the transplant is more likely to be successful. On average, you have a one in four chance of having the same HLA type as a sibling. Many patients, therefore, don't have a sibling with the same tissue type.

If a brother or sister doesn't provide a match, your doctor will search registries of volunteer donors such as theNational Marrow Donor Programfor an unrelated donor that matches your tissue type. A donor who's not related to you but who has a similar tissue type is called a matched unrelated donor (MUD).

Stem cells for transplantation are collected from three sources:

Before stem cells are collected from blood or bone marrow, the donor must undergo a thorough physical exam and blood testing for hepatitis viruses, human immunodeficiency disease (HIV) and other infectious agents or viruses.

The most common source of stem cells for transplant is peripheral blood, the blood that flows throughout our veins and arteries.

Bone marrow normally releases a small number of peripheral blood stem cells (PBSCs) into the bloodstream. To obtain enough PBSCs for a transplant, the donor takes a white cell growth factor, such as granulocyte-colony stimulating factor (G-CSF) drug, which increases the number of stem cells by drawing them out of the marrow and into the bloodstream. When a patient's own stem cells are used, both G-CSF and the chemotherapy used to treat the disease usually increase PBSCs. In patients who have myeloma and non-Hodgkin lymphoma, the drug plerixafor (Mozobil) can be used to mobilize their own stem cells.

The blood is removed from the donor and the cells collected using a process called apheresis, which involves placing a needle in the donor's vein, usually in the arm, similar to administering a blood test. The donor's blood is pumped through an apheresis machine, which separates the blood into four components: red cells, plasma, white cells and platelets. The white cells and platelets, which contain the stem cells, are collected, while the red cells and plasma are returned to the donor. It can take one to two sessions of apheresis to collect enough blood from a MUD. If you are your own donor, it may take more than two sessions.

If enough stem cells can't be retrieved from apheresis, they can be removed directly from the bone marrow. This requires the donor to undergo a minor outpatient surgical procedure.

While the donor is under anesthesia, the surgeon inserts a hollow needle into the donor's pelvic bones just below the waist and removes liquid marrow. This is done a number of times until several pints of marrow are collected. The donor can expect to stay in the hospital for six to eight hours after the procedure to recover from the anesthesia and the acute pain at the needle insertion sites. He or she may feel some lower back soreness for a few days afterward. The donor's body naturally replaces the marrow soon after the procedure. Red cells are also removed, and the donor may experience anemia, which is often treated with iron supplements.

The marrow that's removed (harvested) passes through a series of filters to remove bone or tissue fragments and is then placed in a plastic bag from which it can be infused into the recipient's vein. The marrow is usually given to the patient within a few hours and almost always within 24 hours. If necessary, however, marrow can be frozen and stored and will remain suitable for use for years. If the transplant is autologous, the marrow is usually frozen while the patient undergoes intensive chemotherapy.

A rich source of stem cells for blood cancer patients are the stored stem cells collected from the umbilical cord and placenta after a baby is born, called the cord blood unit. Parents may choose to have the cord blood unit collected after delivery. Healthy parents with healthy children and no transplant candidate in the family can choose to donate their newborn's cord blood to cord blood banks or research programs at participating hospitals. Parents with a child or a family member who could be a candidate for transplantation should discuss with their doctor the potential benefits of saving their newborn's cord blood for possible family use.

Advantages of Using Cord Blood

The advantages of using cord blood stem cells instead of donor peripheral blood or donor marrow stem cells include:

Disadvantages of Using Cord Blood

There can be disadvantages of using cord blood stem cells as well:

The decrease in marrow function often begins to take effect by the second or third day after an allogeneic stem cell infusion. You'll be kept in a protected environment to reduce contact with infectious agents. Generally within two to four weeks after the transplant, the engraftment of donated cells will be apparent from the appearance of normal white cells in your blood. You'll receive periodic transfusions of red cells and platelets until your marrow function has been restored by the transplanted stem cells.

Your doctor will carefully monitor you with physical exams, blood chemistry tests, imaging studies and other tests to ensure that your heart, lungs, kidneys, liver and other major organs are functioning normally. You'll need drugs to prevent GVHD, in addition to blood transfusions. If you're suffering from a poor appetite or diarrhea, you may need to be fed intravenously or through a duodenal tube (called hyperalimentation) to ensure you get adequate nutrition.

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Allogeneic Stem Cell Transplantation | Leukemia and ...

Is This Stem-Cell Clinic Really Making Cancer Vaccines …

By Stem Cell Clinic

Stem cell treatments usually involve sucking out some of a patients fat tissue, isolating the stem cells within, and reinjecting them into the patients body.

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On the first Saturday of March, Kristin Comella put on a white doctors coat and took the stage at the fourth annual conference for the Academy of Regenerative Practices. The founder and president of the academy, Comella also oversees an expanding empire of stem cell clinics that promise patients cures for most anything that ails them. None of those treatmentsfor everything from diabetes and asthma to multiple sclerosis and arthritishave been approved by the US Food and Drug Administration.

The procedurewhich costs a few thousand dollarsis always pretty much the same, regardless of its purported target. It involves sucking out some of a patients fat tissue with a liposuction needle, isolating the stem cells within, and reinjecting them into the patients body. The simplicity of the procedure is why people like Comella say its insane for the FDA to try to regulate stem cells.

So it was surprising when she announced onstage that her firm, US Stem Cell, had recently begun developing a radically new kind of treatmentthis time, for cancer.

Your stem cells are antigen-presenting cells, Cormella told the audience, in a Facebook live video the company posted of the event. We can make them express a protein from your specific cancer. So, its an individualized cancer vaccine, if you will. US Stem Cell, a publicly traded firm that sells stem cell separation kits and operates one of the largest networks of clinics in the country, achieved this with something called an electroporation protocol, she said.

Electroporation is essentially zapping cells with electricitya microbiology technique used to get drugs, proteins, or, most commonly, DNA into cells. When I hear electroporation, thats equal to genetic modification, says Paul Knoepfler, a stem cell researcher at UC Davis. Thats what we do when we want cells to permanently express a protein.

Knoepfler writes a blog about stem cells, and thats where he surfaced the video on May 9, after an acquaintance tipped him off. Hes sort of a watchdog for the industry. Since 2011, hes tracked the proliferation of unregulated stem cell clinics and followed US Stem Cells cavalier approach to experimenting on its patients, sometimes to disastrous effect. In 2015, one of its clinics injected liposuction-derived stem cells directly into the eyeballs of three elderly women suffering from age-related macular degeneration. All three went blind, two sued, and US Stem Cell settled out of court.

But this, he says, might be the most dangerous thing hes seen yet. If my assumption is correct that theyre introducing DNA, this is up near the top of the riskiest things Ive ever heard a stem cell clinic doing, he says. The big worry here is giving cancer patients another cancer, or a dangerous immune response."

US Stem Cell did not respond to WIREDs questions about the procedure, so its still unclear if it does indeed involve genetic modification and whether any patients have actually been treated with it. In the video Comella only described it as one of the companys current protocols.

What we do know is that the approach sounds similar to a powerful new class of anti-cancer medicines known as CAR-T therapies. They involve extracting a patients immune cells and genetically rewiring them to more effectively recognize and attack cancerous cells in the body. The FDA approved the first CAR-T, Kymriah, in late 2017 after scrutinizing years of data from animal studies and human clinical trials. Novartis claims it spent $1 billion to get the treatment to market.

Compare that with the $6,664 US Stem Cell reported having spent last year on research and development. The companyformerly named Biohearthas nine clinical trials listed on the national registry ClinicalTrials.gov, none of which are actively recruiting and none of which are for cancer treatments. Though listed as the lead investigator on some of the trials, Comella isnt a medical doctor. She received a three-year online PhD in stem cell biology from the Panama College of Cell Sciencea non-accredited virtual university founded by stem cell evangelist Walter Drake, according to reporting by the LA Times. And shes not afraid to spar with the federal government.

Last August the FDA sent a warning letter to US Stem Cell and to Comella, specifically, for significant deviations from good practices. And after Comella responded with a letter of her own, denying FDA has any jurisdiction to regulate her companys activities, the agency followed up with a lawsuit.

On May 9, the FDA along with the Department of Justice filed a complaint seeking a permanent injunction against US Stem Cell and Comella, accusing them of endangering patient safety and failing to meet manufacturing standards for cell therapies. The federal officials also filed a similar lawsuit against another clinicCalifornia Stem Cell Treatment Centerwhich was involved in giving patients an experimental cancer treatment made from a mix of stem cells and a smallpox vaccine inappropriately acquired from a Centers for Disease Control and Prevention stockpile under the auspices of research. When officials found out they were being administered to patients, US marshals raided the clinic and seized the remaining vials.

Both California Stem Cell Treatment Center and US Stem Cell said in public statements they plan to fight the injunctions, the most aggressive volley yet in the conflict surrounding direct-to-consumer stem cell treatments. At the heart of the clash is the phrase minimally manipulated, which the FDA uses to exempt therapies like bone marrow transplants. Both clinics will likely argue in court that their cell-based treatments fit that description. But Comellas recent statements at the conference could undermine this claim. Electroporation is a tool designed explicitly for cellular manipulation, and theres nothing minimal about it.

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Is This Stem-Cell Clinic Really Making Cancer Vaccines ...

Anyone have objective data on the effectiveness of the …

By Stell Cell Research

@StemCellPioneers I think you raise an interesting argument, but not for the reasons you list. The FDA is at least trying to base decisions on ACTUAL DATA, whereas there IS NO VERIFIABLE DATA on the therapeutic use of stem cells. What the FDA is saying is that the study is limited, and thus should be interpreted with caution. Therefore, they are being CONSISTENT. Flawed or limited data does not equal legitimate data on which to base decisions. So yes, if a study is limited, according to the FDA, then it is acceptable that a potentially dangerous drug should be continued to be given to children.

I dont have access to this particular study from home, but I can already tell you that retrospective case control studies can have significant problems with bias and confounding. See, this study states in the methods The primary exposure measure was the presence of amphetamine, dextroamphetamine, methamphetamine, or methylphenidate according to informant reports or as noted in medical examiner records, toxicology results, or death certificates. Now, even without reading the paper, which I will, I can already identify a potential source of bias. Kids who experienced sudden death were probably a lot more likely to have an autopsy, where drugs would be found by toxicology, whereas the kids who died in auto accidents probably were less likely to have autopsies, and the investigators likely relied on interviews with the family (less reliable). Thus, I predict that when I read the study tomorrow, I will find more autopsies in the sudden death group, and thus a greater association of sudden death with stimulants (because they were found more frequently by a better method). We shall see.

In any event, at least the FDA is weighing DATA, pros and cons, etc. There have been many cases of the FDA pulling medications and-or issuing black box warnings (for example, the diet drug fen-phen). This is how the system works. No drug is perfect, and as such needs to be approved through prospective studies, and constantly reviewed via aftermarket analysis. If more proof accumulates indicating that stimulants are harmful in ADHD, you can be sure the FDA will pull the drugs from the market. In contrast, the stem cell pioneers are just pushing forward, without so much as a hint that there could be some benefit (save lots and lots of anecdotal stories, which could easily be attributed to placebo affects).

I will say this again (perhaps for the 5th or 6th time). As a physician-scientist I truly believe in the promise of stem cells. I really, really do. However, no one will ever know if stem cell treatments are effective if they arent studied in rigorous, well-controlled clinical trials. This is true of all new treatments. And I would ask, why are the purveyors of this treatment not the leaders in the field of stem cell research? You talk about reputable stem cell clinic or doctor, but I consider that a misnomer. No reputable doctor would perform these infusions without it being part of a clinical trial or as an already verified procedure. Furthermore, this thread has piqued my interest in this area, and Ive been doing a lot of web research over the past few days. Interestingly, quite a few of the doctors and clinics discussed on your very site have had major legal and ethical issues (including doctors that are heavily promoted), which argues against them being reputable. The doctors who run these clinics may claim to be scientists, but in reality, the bulk of cutting edge stem cell work is going on in major academic hospitals, not small, private clinics run by doctors with little to no scientific training.

Finally, I really hope you find what you are looking for in stem cell infusions. Neither I nor any doctor wants patients to suffer needlessly, but apparently the majority of the medical community views the current status of stem cell treatments to be in its infancy. Many of us are concerned that the quixotic pursuit of highly experimental stem cell treatments could be detrimental on an emotional, financial, and possibly biologic level.

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Anyone have objective data on the effectiveness of the ...

Learn Zone – VetCompass – Royal Veterinary College, RVC

By Stell Cell Research

Veterinary Epidemiology in Practice - The VetCompass Programme

Dr. Dan O'Neill (VetCompass, RVC)

In this e-lecture, recorded as part of the VET Talks series hosted by the RVC, Dr Dan O'Neill gives an overview of practice-based veterinary epidemiological research and describes the important role of VetCompass in pushing the boundaries of this exciting new field.

Dr. Dan O'Neill (VetCompass, RVC) & Dr. Katy Evans (University of Nottingham) British Small Animal Veterinary Association Annual Congress, 2015

This talk was delivered at BSAVA Congress 2015 and addresses the importance of generating high quality evidence to inform decision-making for the improvement of canine welfare. Dr. Dan ONeill and Dr. Katy Evans discuss the importance of evidence-based veterinary advice when aiming to improve dog health at a population level, highlighting how large-scale, ongoing health surveillance projects such as VetCompass are vital in providing relevant, representative findings for practical use by clinicians.

This audio recording is shared by kind permission of the UK Kennel Club.

Dr. Dan O'Neill (VetCompass, RVC) & Aimee Llewellyn (Geneticist & Health Information Manager, UK Kennel Club)British Small Animal Veterinary Association Annual Congress, 2015

This talk was delivered as part of the first ever BSAVA lecture stream on Practical aspects of dog breeding. Dr. Dan ONeill and Aimee Llewellyn (of the Royal Veterinary College & UK Kennel Club respectively) presented information on the practical approaches veterinary practices can take to improve the advice they give to breeder clients. Bothspeakers emphasised the vital role that veterinary practitioners can play in improving dog health at a population level and highlighted the importance of large-scale, ongoing health surveillance projects such as VetCompass.

This audio recording is shared by kind permission of the UK Kennel Club.

Discussinghowwe canuse the information contained in veterinary clinical records to better understand pain-related welfare in companion animals

A short video about VetCompass with examples of evidence generated, with musical accompaniment (no speaker)

Information on the expected lifespan and causes of death in dogs in England based on a VetCompass Programme study

Find out how common epilepsy is in dogs and which breeds are affected

McGreevy, PD, Wilson BJ, Mansfield, CS.Church DB, Brodbelt DC, Dhand, N,Soares Magalhaes, RJ and O'Neill DG. (2018)Canine Genetics and Epidemiology

O'Neill DG, Baral L, Church DB, Brodbelt DC and Packer RMA (2018) Canine Genetics and Epidemiology 5:3.

O'Neill DG, Darwent EC, Church DB and Brodbelt DC (2017) Canine Genetics and Epidemiology 4:15

O'Neill DG, Yin Seah W, Church DB and Brodbelt DC (2017) Canine Genetics and Epidemiology 4:13

O'Neill DG, Coulson NR, Church DB and Brodbelt DC (2017) Canine Genetics and Epidemiology 4:7

O'Neill DG, Darwent EC, Church DB andBrodbelt DC (2016) Canine Genetics and Epidemiology, 3(1):1-12.

Summers JF, ONeill DG, Church DB, Thomson PC, McGreevy PD and Brodbelt DC. (2015) Canine Genetics and Epidemiology.

Boyd, C., Jarvis, S., McGreevy, P., Heath, S., Church, D., Brodbelt, D., and O'Neill, DG. (2018)Animal Welfare

Conroy, M., O'Neill, DG., Boag, A., Church, DB., and Brodbelt, DC. (2018). Journal of Small Animal Practice.

McDonald JL, Cleasby LR, Brodblet DC, Church DB and O'Neill DG (2017) Journal of Small Animal Practice DOI: 10.1111/jsap.12716, n/a-n/a. (Early view)

O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC (2014) Veterinary Journal.

O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC (2014) Journal of Feline Medicine and Surgery.

O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC(2014) PLoS One,9(3).

O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC(2013) The Veterinary Journal,198,638-643.

Mattin MJ, Boswood A, Church DB, Brodbelt DC (2018) Journal of Veterinary Internal Medicine

Mattin MJ, Boswood A, Church DB, McGreevy PD, O'Neill DG, Thomson PC, Brodbelt DC. (2015; Epub ahead of print) Preventive Veterinary Medicine

Mattin MJ, Boswood A, Church DB, Lpez-Alvarez J, McGreevy PD, O'Neill DG, Thomson PC, Brodbelt DC. (2015) Journal of Veterinary Internal Medicine

O'Neill DG, Gostelow R, Orme C, Church D., Niessen SJM, Verheyen K & Brodbelt DC (2016) Journal of Veterinary Internal Medicine

O'Neill DG, Scudder C, Faire JM, Church DB, McGreevy PD, Thomson PC andBrodbelt DC(2016)Journal of Small Animal Practice2016

Mattin MJ, O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC (2014) The Veterinary Record,174(14), 349.

Stephens MJ, O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC (2014) The Veterinary Record.

O'Neill DG, Case J, Boag AK, Church DB, McGreevy PD, Thomson PC & Brodbelt DC (2017) Journal of Small Animal Practice, DOI: 10.1111/jsap.12723, n/a-n/a

Erlen A, Potschka H, Volk HA, Sauter-Louis C, O'Neill DG, (2018) Journal of Veterinary Internal Medicine.

Kearsley-Fleet L, O'Neill DG, Volk HA, Chursh DB, Brodbelt DC (2013) The Veterinary Record;30;172

O'Neill, DG., Corah, CH., Church, DB., Brodbelt, DC., and Rutherford, L. (2018).Canine Genetics and Epidemiology

Shoop SJ,Marlow S,Church DB,English K,McGreevy PD,Stell AJ,Thomson PC,O'Neill DGandBrodbelt DC (2014) Canine Genetics and Epidemiology.

O'Neill, D.G., Lee, M.M, Brodbelt, D.C., Church, D.B. & Sanchez, R.F. (2017) Canine Genetics and Epidemiology 4:5

Anderson KL, O'Neill DG, Brodbelt DC, Church DB, Meeson RL, Sargan D, Summers JF, Zulch H & Collins LM(2018)Scientific Reports

O'Neill DG, Meeson RL, Sheridan A, Church DB andBrodbelt DC (2016) Canine Genetics and Epidemiology

Taylor-Brown FE, Meeson RL, Brodbelt DC, Church DB, McGreevy PD, Thomson PC & O'Neill DG. (2015) Veterinary Surgery

O'Neill D, Jackson C, Guy J, Church D, McGreevy P, Thomson P. & Brodbelt D.(2015) Canine Genetics and Epidemiology

O'Neill, D.G., O'Sullivan, A.M., Manson, E.A., Church, D.B., McGreevy, P.D., Boag, A.K. and Brodbelt, D.C. (2019) Veterinary Record

Stevens K.B., O'Neill D.G., Jepson R., Holm L.P., Walker D.J., andCardwell J.M.(2018) Veterinary Record

Hall, J.L., Owen, L., Riddell, A., Church, D.B., Brodbelt, D.C., and O'Neill D.G., (2018)Journal of Small Animal Practice.

O'Neill D.G., O'Sullivan AM, Manson EA, Church DB, Boag AK, McGreevy PD and Brodbelt D.C. and (2017)VeterinaryRecordDOI:10.1136/vr.104108 DOI:10.1111/jsap.12731

O'Neill D.G., Riddell A., Church D.B., Owen L., Brodbelt D.C. and Hall J.L. (2017) Journal of Small Animal Practice DOI:10.1111/jsap.12731

O'Neill DG, Elliott J, Church DB, McGreevy PD, Thomson PC, Brodbelt DC(2013) Journal of Veterinary Internal Medicine;27(4):814-21

Buckland, E., O'Neill, D., Summers, J., Mateus, A., Church, D., Redmond, L. and Brodbelt, D. Veterinary Record (2016) doi:10.1136/vr.103830

Summers JF, Hendricks A, Brodbelt DC (2014) BMC Veterinary Research.

O'Neill DG, Hendricks A, Summers JF,Brodbelt DC(2012) J Small Anim Pract;53(4): 217-22

Muellner, P., Muellner, U., Gates, M. C., Pearce, T., Ahlstrom, C., O'Neill, D., Brodblet, D. & Cave, N. J. (2016) Frontiers in Veterinary Science, 3.

O'Neill DG, Church DB, McGreevy PD, Thomson PC, Brodbelt DC (2014) Canine Genetics and Epidemiology,1:2.

Hoffman, J.M., Creevy, K.E., Franks, A., O'Neill, D.G. and Promislow, D.E.L. (2018) Aging Cell.

Hoffman, J.M., O'Neill, D.G., Creevy, K.E., & Austad, S.N.(2018)The Journals of Gerontology: Series A, 73, 150-156.

Jin, K., Hoffman, J.M., Creevy, K.E., O'Neill, D.G. and Promislow, D.E.L. (2016) Pathobiology of Aging and Age-related Diseases6:33276

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Learn Zone - VetCompass - Royal Veterinary College, RVC

Does Medicare Cover Platelet-rich Plasma Injections

By Platelet Rich Plasma Injections

Modern blood pressure may discovered. It works especially if you have a busy lifestyle now. She could not take the shoulder will be much more results are visible as scientists believe there is a deficiency.

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platelet Dengue is another common factors in acute myelogenous compounds that help keep your arteries to heal and also the lymph system. The complete view that COX-2 drugs.

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http://jem.rupress.org/content/187/3/329.full http://lowplatelet.net/overproduction-of-platelets-by-megakaryocytes/ http://jpet.aspetjournals.org/content/228/1/240 http://iai.asm.org/content/78/1/413.full.pdf http://www.freepatentsonline.com/5817519.html http://lowplatelet.net/how-to-build-up-platelets-in-the-blood/

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Does Medicare Cover Platelet-rich Plasma Injections

Retinitis Pigmentosa – The Foundation Fighting Blindness

By Stell Cell Research

Overview

Retinitis pigmentosa (RP) describes a group of genetic disorders that damage light-sensitive cells in the retina, leading to gradual vision loss over time as the cells die off. While the condition is classified as a rare disease, it is one of the most common inherited diseases of the retina, affecting between 1 in 3500 to 1 in 4000 Canadians.[1]RP is often referred to as an inherited retinal disease, meaning that it is passed along genetic lines and inherited from ones parents. Though it is usually diagnosed during childhood or adolescence, a minority of patients report symptoms later in life.

Specialized cells called photoreceptors are responsible for absorbing light and translating it into signals that are interpreted by the brainit is these essential cells that gradually die off as a result of RP. The cells come in two varieties: rod cells and cone cells. Rod photoreceptors are responsible for peripheral and night vision, while cone photoreceptors are responsible for central, high-acuity vision as well as detail and colour. Since it is the rod cells that are first damaged by RP, peripheral and night vision are affected during the early stages of the disease, followed by a narrowing of the visual field, often referred to as a progressive form of tunnel vision. The death of rod cells eventually affects the cone cells as well, leading to the loss of central vision and often resulting, during the later stages of the disease, in near or total blindness. The length of this process varies from individual to individual.

RP was originally considered a single disease, but after decades of researchincluding research funded by the FFBwe now know that there are several forms of RP, and that these forms involve mutations in any one of more than 64 different genes. The gene or genes affected determine the disease type and symptoms.

There are several different ways that RP can be inherited, which is usually described as the inheritance pattern. The different RP inheritance patterns include: autosomal dominant, autosomal recessive, and x-linked recessive. A genetic counsellor can talk with you about your family history and determine which of these patterns is associated with your vision loss. With this information, the genetic counsellor may be able to tell you more about how your condition will progress, and give you and your family information about the risks of vision loss for other family members. To learn more about genetic testing for RP, please consult the FFB resource Everything You Need to Know about Genetic Testing.

Typically, each person with RP only has damage in one pair of genes. Scientists have now identified more than 64 genes that can have mutations that cause RP. It is likely that mutations in more than 100 different genes will eventually be identified. Because so many RP-causing gene mutations are still unknown, there is about a 50:50 chance that genetic testing will provide a definitive result. Given your family history and the inheritance pattern of your RP, your genetic counsellor will be able to advise you about the likelihood that a genetic test will provide a definitive result.

Different genetic mutations can damage the retina or impair its function in different ways; for example, some mutations affect how the retina processes nutrients, while others damage the photoreceptors. Its important to identify the specific gene and mutation, because many treatments being developed for RP will be for particular genetic types.

Content on this page was written by Dr. Chad Andrews and Dr. Mary Sunderland, and was most recently updated on August 23, 2018. An earlier version of the content was approved by Dr. Jane Green and Dr. Bill Stell.

The most common early symptom of RP is difficultly seeing at night and in low-light conditionsthis is called nyctalopia or night blindness. The loss of peripheral vision is also a common first symptom, and is often experienced alongside nyctalopia. As RP progresses, peripheral vision slowly diminishes, resulting in a narrow field of view or tunnel vision. By age 40, many people with RP are legally blind, with a severely constricted field of vision, although many may retain the ability to read and recognize faces. Uncomfortable sensitivity to light and glare is common, as is photopsia (seeing flashes of light or shimmering). RP can also cause a loss of visual acuity (the ability to see clearly), but the onset is more variable. Some patients retain normal visual acuity, even when their vision is reduced to a small central island; others lose acuity much earlier in the course of disease. Eventually, however, most people with RP will begin to lose central vision and some will lose all light perception.

An ophthalmologist may suspect RP on the basis of a persons symptoms and the findings of a simple eye examination. Two tests are used to clarify the diagnosis:

Currently, there is only a single approved treatment for a very rare form of RP on the market in the United States: a gene therapy called Luxturna, which can halt vision loss and even restore some sight in individuals with a biallelic mutation of their RPE65 gene (manifesting as either RP or Leber congenital amaurosis). Though the number of patients with this mutation is small, the medical effectiveness of Luxturna and its materialization as a pharmaceutical product demonstrate that there is significant potential for gene therapy to treat other forms of RP in the future.

Read Our Story About The Approval of Luxturna

Clinical trials are essential to the scientific process of developing new treatments: they test the viability and safety of experimental drugs and techniques, called interventions, on human beings. While there is no guarantee that enrolling in a clinical trial will provide any medical benefit, some patients do experience positive results after receiving an experimental therapy.

Read Our Clinical Trials Guide

The website clinicaltrials.gov is a centralized database of clinical trials that are offered globally. But as the disclaimer on the sites home page states, there is no guarantee that a listed trial has been evaluated or approvedthe National Institutes of Health runs the site but does not vet its content. This means that there could be bogus or dangerous trials listed that are preying on patients. It is essential that you discuss a clinical trial with your ophthalmologist before enrolling, and that you pay close attention to enrollment criteria.

If you are interested in exploring what is available on the site you can click on the button below, which will take you to clinicaltrials.gov and initiate a search for trials relevant for patients living with RP.

CLINICAL TRIALS FOR Retinitis Pigmentosa

For individuals living with an inherited retinal disease (a disease caused by a genetic mutation), participation in a clinical trial could be a logical next-step (for a description of clinical trials, see above). But in Canada there is no centralized, guided mechanism for enrolling in a trial; with this in mind, the Foundation Fighting Blindness has developed a secure medical database of Canadian patients living with inherited retinal diseases: we call it the Patient Registry.

By enrolling in the Patient Registry, your information will become a part of this essential Canadian database that can be used to help connect you to a relevant clinical trial. The availability of relevant trials depends on a number of factors, so this tool provides no guarantees, but signing onto it will put you in a position to be connected to something appropriate. It is also a way of standing up and being counted: the more individuals enrolled in the Patient Registry, the better our chances of showing policymakers that there is a significant need for new treatments for inherited retinal diseases. The Patient Registry also helps to drive more sight-saving research!

You can begin the process of enrolling in the Patient Registry by clicking the button below.

Patient Registry Enrollment

The Foundation Fighting Blindness is committed to advancing the most promising sight-saving research, and has invested over $33 million into cutting-edge science since the organization was founded. Recognizing that science is tied to policy frameworks, the Foundation is also actively involved in health policy activities across Canada.

Many research groups are working to develop treatments and cures for RP. Experimental treatments can be divided into three broad categories:

Protective therapies aim to stop (or at least slow) the damage caused by genetic mutations. Often protective therapies are not specific to one mutation, but may benefit people with many types of RP. These include treatments to stop the process of photoreceptor death (apoptosis), as well as cell-derived therapies that aim to help photoreceptors survive.

Some protective therapies aim specifically to prevent the death of cone cells in RP and thus, the loss of central vision in later stages of the disease.

Corrective therapies aim to reverse the underlying genetic defect that causes vision loss. If these therapies are successful they might prevent a person who is treated when first diagnosed, from ever developing vision loss. Corrective therapies might also help slow the disease in people whose vision has already been affected, especially in the earlier stages. The corrective therapies being developed now are specific to certain forms of recessively inherited RP. Gene therapies, which replace a non-functioning gene, are one type of corrective therapy. Clinical trials of gene therapies for several types of RP are underway, and the results so far are encouraging.

Sight-restoring therapies are also a growing area of research success. These therapies are intended for people who have already lost all, or much, of their vision. Stem cell therapies aim to replace the retinas lost photoreceptors. There are promising early results with stem cell trials involving other retinal degenerative diseases; trials with RP are on the horizon. Retinal prosthetics, such as the Arugus II or Bionic Eye, use computer technology to generate vision. The Foundation Fighting Blindness helped to support the first Canadian trial of the Argus II and continues to work closely with health policy experts across Canada to ensure that patients who could benefit from the Argus II device have access to this innovative treatment. Drug and gene therapies are also being developed that may give non-photoreceptor nerve cells in the retina the capacity to sense light.

Thanks to our generous donors, we are funding ground-breaking research in these areas. Click on the button below to review the full list of FFB-funded projects:

FFB-FUNDED RESEARCH

On the right side of this webpage, you will find an updating list of stories that detail new research and health policy developments relevant for individuals affected by RP.

The page you are now on provides information on RP, but the Foundation Fighting Blindness has developed additional resources that can be helpful in plotting an optimal path through vision care. Below is a list of such resources, including information on genetic testing, clinical trials, Vision Quest (the FFBs in-person educational events), and more. The list will update as new resources are added.

Must-Read Resources Vision Quest Educational Series

We know that helpful resources related to your eye disease can be difficult to find. Vision care in Canada entails a complex web of services, programs, and instructions, and little of it is centralized. The information on this site represents our attempt at providing a comprehensive, centralized resource that offers guidance and information specific to your eye disease. Our goal is to help you find your optimal path through vision care in Canada, which is why we call this initiative Vision Care Pathways.

December 12th, 2018 by FFB Canada

Right now, over 1 million Canadians are living with blinding eye diseases and as vision fades, so too can hope. To date, donors of the Foundation Fighting Blindness (FFB) have contributed more than $32 million for vision research. And now, until the end of 2018, a generous supporter will match your gift up to a

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November 13th, 2018 by FFB Canada

On Saturday, October 20, 2018, family and friends of the Celebres came together in support of one very special little boy. Nicholas Celebre was born with Usher syndrome,a condition that causes deaf-blindness and often balance issues. Born profoundly deaf, he was fortunate enough to get cochlear implants when he was 12 months old. He also

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November 13th, 2018 by FFB Canada

Guest-written by Deborah Scott. Our daughter, Olivia was 5 years old when she was diagnosed with a blinding eye disease called retinitis pigmentosa (RP). It was difficult for us to comprehend what that diagnosis really meant. As a parent, you never get over the impact of learning that there is so much more to vision

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Retinitis Pigmentosa - The Foundation Fighting Blindness

Everything You Need To Know About The Bionic Eye – The …

By Stell Cell Research

May 18th, 2016 by FFB Canada

Click here to download a printable version this article/fact sheet: PDF/Word.

What is a retinal prosthesis? A retinal prosthesis is a non-living, electronic substitute for the retina. Popular and brand names for retinal prostheses are the Bionic Eye and the Argus II. The aim is to restore vision to someone blinded by retinal eye disease. A retinal prosthesis is different from an implanted lens or a low-vision device, which works to maximize a persons existing vision.

What does it do? In people with advanced retinal disease, the light-capturing cells of the retina, called photoreceptors, have been lost, but the network of nerves that sends visual information to the brain often is intact. A retinal prosthesis bypasses the photoreceptors and sends visual signals to the brain.

Will it restore my vision? The prostheses that have been tested so far do not provide natural sight. For example, people who are using them are able to recognize a doorway or the shape of a person, or in some cases can make finer distinctions, such as the difference between a fork and a spoon. These retinal prostheses provide a simulation of sight which means that the users have to re-learn how to see. Their brains need to learn how to interpret this new kind of information.

Who could use it? Retinal prostheses are intended for people who are blind or have only minimal light perception, but who once had sight. With prostheses, the brain must interpret the devices signals. Someone blind from birth never developed this capacity, and therefore it might not benefit them.

Are any approved in Canada? Yes. The Argus II Retinal Prosthesis is approved by Health Canada. It is also approved in Europe and the USA. The Foundation Fighting Blindness played a key role in bringing the Argus II (sometimes called the Bionic Eye) to Canada by helping to fund an observational clinical trial of the device at the Toronto Western Hospital led by Dr. Robert Devenyi.

What will it cost? The Argus II Retinal Prosthesis is now being marketed in Europe for about $100,000 USD, plus the cost of the surgery to implant it. Second Sight (the manufacturer) is actively seeking coverage of the device through public insurance or government subsidies. The costs of other retinal prostheses are not yet known.

How does it work? Just as there are multiple kinds of smart phones, there are different approaches to this technology.

Camera + Epi-Retinal Chip The Argus II by Second Sight is the leader in this category. It captures images with a mini-camera embedded in glasses that also carry a batterypack. A 2D array of many tiny electrodes is implanted surgically on the front surface of the retina (epi-retinal). Images from the camera are converted into electrical pulses sent wirelessly to the implant. The pulses stimulate the retinas remaining cells to send patterns of nerve impulses, representing the images, along the optic nerve to the brain. Patients can learn to interpret the patterns and regain some functional vision. Most of the people who have received an Argus II implant have had some visual perception restored, allowing them to better orient themselves in a room or negotiate daily tasks. There appear to be significant variations in results between users.

The Intelligent Retinal Implant System is another camera/chip combo, similar to the Argus II. It is in clinical trials in Germany and the UK. Bionic Vision Australia is also working on a similar product.

Sub-Retinal Chip Retinal Implant AG has created a sub-retinal implant, which sits behind the retina instead of in front of it. This electronic chip contains tiny photocells to capture light, amplifiers to boost their signal, and electrodes to stimulate retinal nerve cells. Since photocells are part of the chip, the device does not need an external camera, and the sub-retinal placement should be more secure and stable than the epi-retinal option; but morecomplicated surgery is required to implant it. Clinical trials of this device are ongoing in Germany, Italy and the UK, and in the USA.

Other groups developing chips include Artificial Silicon Retina Microchip, the Boston Retinal Implant Project, and Nano Vision although the later two are not yet at the human trial stage.

Sub-retinal chips may allow somewhat higher resolution images than epi-retinal chips. However, since even the tiniest electrodes in these prostheses are bound to stimulate more than one retinal cell, so the wearers visual acuity may never approach normal sight. This limitation has led to hybrid strategies, in which remaining retinal nerve cells are made light-sensitive andthen stimulated by patterns of light instead of electricity.

Encoding Neural Signals Dr. Sheila Nirenberg of Cornell University is one of several researchers, who are developing this new hybrid approach to prosthetics. In Dr. Nirenbergs studies, a camera sends images to a computer, which measures local differences in intensity across the image and encodes this information in pulses of light that mimic the natural language of the central nervous system. The size of these pulses of light can be smaller than the smallest retinal nerve cells; they can be projected through the pupil onto individual retinal cells. Using a new approach called optogenetics, a form of gene therapy endows these nerve cells with the ability to respond directly to light, so that the computer-generated light pulses stimulate them to send high-resolution, realistic image representations to the brain. This approach is being tested in animals. If it proves to be effective, it should provide much higher-quality images and a more natural visual experience. Dr. Gautam Awatramani at the University of Victoria is one scientist funded by the Foundation Fighting Blindness donors to study similar therapies.

Direct to Brain Prothesis Scientists at the Monash Vision Group in Australia have developed a different type of vision prosthesis. It avoids the retina altogether. This device uses a video camera to capture images and send its electronic signals directly to the visual cortex of the brain.

While brain surgery sounds like a more difficult, and risky option, the surgery required is relatively straightforward. More importantly, if it is successful, the device could have some important advantages. For example, it could help people with retinal degenerative disease, but it might also help people whose optic nerve has been damaged due to glaucoma or injury

As well, this prosthesis would not be implanted into the retina and thus would not block or damage retinal tissue. So the prosthetic could be used to augment vision for people with some remaining sight, and would not impair their remaining vision. The Monash Vision Group and its partners have committed to having their direct to brain bionic eye ready for first patient tests very soon.

Updated May 18, 2016: Dr. Mary Sunderland, Director of Research & Education, Foundation Fighting Blindness. Initially reviewed by Dr. Bill Stell, Professor of Cell Biology and Anatomy, at the University of Calgary and Dr. Gautam Awatramani, University of Victoria.

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Everything You Need To Know About The Bionic Eye - The ...

Pluripotent stem cells for improved reprogrammed Human …

By Induced Pluripotent Stem Cells

Following the start of our recent collaboration with Phenocell, were pleased to be able to provide high quality Sebocytes developed from Human induced pluripotent stem cells (iPSC). Thanks to a perfectly standardized reprogramming protocol, they display lower batch to batch variability, allowing better reproducibility and accuracy of your experimental results.

Sebocytes have demonstrated their large potential to be unique tools for many life science research fields such as:

These cryopreserved reprogrammed pluripotent stem cells are available at low passage (P2), 2.106 cells/vial format and in 3 different phototypes (Caucasian, Asian and African). Developed from highly qualified Human iPS cells, each lot is validated, with a specific certificate of analysis, for all the following Sebocyte markers and specific functions.

Phenocells iPS-derived human Sebocytes display the typical epithelial morphology of primary sebocytes with heterogeneity in cell size due to lipid accumulation.

Phenocell Sebocytes from human induced pluripotent stem cells.

Expression of the two Sebocyte markers: MUC1 expressed in more than 80% of cells; KRT7 expressed in 80% of cells

Functional markers are strongly expressed after 5 days:

Evolution of specific markers in Sebocytes derived from Human induced pluripotent stem cells after 3 (d3) and 5 (d5) days in culture with the specific PhenoCULT-SEB culture medium, compared to primary keratinocytes (Ker).

KRT7 expression shows Sebocyte purity above 90%.

Black: isotype control; Red: anti-KRT7 antibody

Dose-dependent (up to 5-fold) lipid accumulation (Bodipy staining), response after a 24hr treatment with linoleic acid (LA)

PCi-SEB respond to a 96hr treatment with testosterone (10 M) by a 2-fold increase in lipid content. This response is significantly inhibited by the 5-reductase inhibitor Finasteride (10 M).

Interested in these Sebocytes developed from Human induced pluripotent stem cells ?

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Induced Pluripotent Stem Cell Market Is Expected to Reach US …

By Induced Pluripotent Stem Cells

New York, NY -- (SBWIRE) -- 02/02/2019 -- The healthcare industry has been focusing on excessive research and development in the last couple of decades to ensure that the need to address issues related to the availability of drugs and treatments for certain chronic diseases is effectively met. Healthcare researchers and scientists at the Li Ka Shing Faculty of Medicine of the Hong Kong University have successfully demonstrated the utilization of human induced pluripotent stem cells or hiPSCs from the skin cells of the patient for testing therapeutic drugs.

To know key findings Request Sample Report @: https://www.persistencemarketresearch.com/samples/17968

The success of this research suggests that scientists have crossed one more hurdle towards using stem cells in precision medicine for the treatment of patients suffering from sporadic hereditary diseases. iPSCs are the new generation approach towards the prevention and treatment of diseases that takes into account patients on an individual basis considering their genetic makeup, lifestyle, and environment. Along with the capacity to transform into different body cell types and same genetic composition of the donors, hiPSCs have surfaced as a promising cell source to screen and test drugs.

In the present research, hiPSC was synthesized from patients suffering from a rare form of hereditary cardiomyopathy owing to the mutations in Lamin A/C related cardiomyopathy in their distinct families. The affected individuals suffer from sudden death, stroke, and heart failure at a very young age. As on date, there is no exact treatment available for this condition. This team in Hong Kong tested a drug named PTC124 to suppress specific genetic mutations in other genetic diseases into the iPSC transformed heart muscle cells. While this technology is being considered as a breakthrough in clinical stem cell research, the team at Hong Kong University is collaborating with drug companies regarding its clinical application.

The unique properties of iPS cells provides extensive potential to several biopharmaceutical applications. iPSCs are also used in toxicology testing, high throughput, disease modeling, and target identification. This type of stem cell has the potential to transform drug discovery by offering physiologically relevant cells for tool discovery, compound identification, and target validation. A new report by Persistence Market Research (PMR) states that the global induced pluripotent stem or iPS cell market is expected to witness a strong CAGR of 7.0% from 2018 to 2026. In 2017, the market was worth US$ 1,254.0 Mn and is expected to reach US$ 2,299.5 Mn by the end of the forecast period in 2026.

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Customization to be the Key Focus of Market Players

Due to the evolving needs of the research community, the demand for specialized cell lines have increased to a certain point where most vendors offering these products cannot depend solely on sales from catalog products. The quality of the products and lead time can determine the choices while requesting custom solutions at the same time. Companies usually focus on establishing a strong distribution network for enabling products to reach customers from the manufacturing units in a short time period.

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Entry of Multiple Small Players to be Witnessed in the Coming Years

Several leading players have their presence in the global market; however, many specialized products and services are provided by small and regional vendors. By targeting their marketing strategies towards research institutes and small biotechnology companies, these new players have swiftly established their presence in the market.

About Persistence Market Research Persistence Market Research (PMR) is a third-platform research firm. Our research model is a unique collaboration of data analytics and market research methodology to help businesses achieve optimal performance. To support companies in overcoming complex business challenges, we follow a multi-disciplinary approach. At PMR, we unite various data streams from multi-dimensional sources. By deploying real-time data collection, big data, and customer experience analytics, we deliver business intelligence for organizations of all sizes.

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Pluripotent Stem Cell Flow Kit (FMC001): R&D Systems

By Induced Pluripotent Stem Cells

H/M Pluripotent Stem Cell Multi-Color Flow Cytometry Kit Summary Kit Summary For the verification of stem cell pluripotency using four established markers. Key Benefits

Identifying the sources of experimental variability is an important consideration in stem cell research where experiments are costly and time-consuming. A potential source of significant variability arises from the starting population of stem cells which can undergo phenotypic changes in culture. SeeDetails

Changes in stem cell potency over time can give rise to large inter-assay errors and/or contradictory data. The Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit offers users an efficient and quantitative method to verify the pluripotency of cells by flow cytometry. Data obtained using this kit can identify and minimize experimental errors introduced by variations in the starting population of cells.

The Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit includes four fluorochrome-conjugated primary antibodies, isotype controls and buffers to fix, permeabilize, and wash cells. SeeDetails

Store at 2 C to 8 C in the dark. Use within 6 months of receipt.

Verification of Human BG01V Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. BG01V human embryonic stem cells were stained using reagents included in the Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit (Catalog# FMC001). Cells were simultaneously analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometry data shows that 91.9% of BG01V human embryonic stem cells are positive for both Oct-3/4 and SSEA4 expression. B. Flow cytometry data shows that 88.5% of BG01V human embryonic stem cells are positive for SSEA-4 and negative for SSEA-1, a phenotype consistent with human embryonic stem cells. C. Flow cytometric analysis shows that BG01V human embryonic stem cells express the pluripotent marker SOX2.

Verification of Mouse D3 Embryonic Stem Cell Pluripotency by Multi-Color Flow Cytometry. Mouse D3 embryonic stem cells were stained using reagents included in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit (Catalog#FMC001). Cells were analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometric analysis shows that 91.1% of mouse D3 embryonic stem cells are positive for both Oct-3/4 and SSEA1 expression. B. Flow cytometric analysis data shows that 82.6% of mouse D3 embryonic stem cells are positive for SSEA-1 and negative for SSEA-4 a phenotype consistent with mouse embryonic stem cells. C. Flow cytometric analysis shows that mouse D3 embryonic stem cells express the pluripotent marker SOX2.

BG01V human embryonic stem cells are licensed from ViaCyte, Inc.

Stability & Storage

Store the unopened product at 2 - 8 C. Do not use past expiration date.

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.

R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits.

Alternate Names

Pluripotent Stem Cells

WARNING: This product can expose you to chemicals including formaldehyde and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to http://www.P65Warnings.ca.gov.

Refer to the product datasheet for complete product details.

Reagents Supplied in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit (Catalog # FMC001)

Intracellular Staining Protocol with Simultaneous Fixation/Permeabilization

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