Stem Cell Clinic

Harvard researchers have successfully created human pain in a petri dish

By Stem Cell Doctors

Its not easy to develop new treatments for a disease. In fact, many of the things doctors try never make it past the early stages. This kind of trial and error takes time, but sometimes patients dont have time to wait around for the next big breakthrough. Thats why having a model for safely studying the human body is ideal. You can do a lot of things to a petri dish that you couldnt do to a living, breathing human, and now that petri dish can feel pain. Researchers at the Harvard Stem Cell Institute (HSCI) have created pain sensing neurons outside the human body.

This recently published studyis the culmination of years of intensive research. The project originally began with embryonic stem cells derived from human embryos. These cells are undifferentiated, meaning they have the potential to become any cell type in the body (often called pluripotent). However, the team has issues getting these cells through all the developmental stages to become adult neurons. Just when all seemed lost, there was a breakthrough in stem cell research. A different team developed induced pluripotent stem cells (resulting in a 2012 Nobel Prize), which is just what the HSCI project needed.

An induced pluripotent stem cell begins as a regular skin cell called a fibroblast. By manipulating the cell, researchers can rewind genetic time to make it into a stem cell, then allow it to develop into a completely different cell type. This is how the HSCI team finally found success. Using fibroblasts from mice and humans, the team evaluated all the developmental steps involved to make their pain sensing neurons. The key was the discovery of new transcription factors that cause cells to produce different proteinsessentially flipping on different parts of the genome that make it a neuron.

The neurons created by the HSCI researchers behave just like real human cells that developed the old fashioned way. They can detect the acute pain associated with stubbing your toe or being pricked by a needle, but more importantly, they also pick up on more subtle signals as with chronic pain or inflammation. This is of particular importance in pain disorders because everyone experiences pain differently and theres only so much a physical examination of the nervous system will tell you.

Going with induced pluripotent stem cells wasnt just a way to reach the goal line. This also makes the resulting model more useful. Patients with chronic neuropathic pain donated fibroblastsfor this study, and the neurons created from those cells ended up with the same neuropathies and hypersensitivity seen in the patients own neurons. So not only do you have a piece of the human nervous system in a dish, you have a model of disease in a real person.

Other teams are sure to begin duplicating this research to better understand human disease, and it will be invaluable to analyze pain sensing neurons outside the body. After all, a flesh and blood person will only put up with so much poking and prodding.

Now read:The 3D printed living bandage that could revolutionize skin grafts

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Harvard researchers have successfully created human pain in a petri dish

Blistering skin disease may be treatable with 'therapeutic reprogramming,' researchers say

By Stem Cell Medicine

PUBLIC RELEASE DATE:

26-Nov-2014

Contact: Krista Conger kristac@stanford.edu 650-725-5371 Stanford University Medical Center @sumedicine

Induced pluripotent stem cells made from patients with a form of blistering skin disease can be genetically corrected and used to grow back healthy skin cells in laboratory dishes, researchers at the Stanford University School of Medicine have found. They've termed the new technique "therapeutic reprogramming."

The skin cells formed normal human skin when grafted onto the backs of laboratory mice, they said.

The findings represent a major advance in the battle against the disease, epidermolysis bullosa, in which the top layer of skin, called the epidermis, sloughs off with the slightest friction, leaving open wounds that are difficult to heal. Severely stricken children who survive into their late teens or early 20s often die from invasive squamous cell carcinoma, a skin cancer that can arise during repeated cycles of skin wounding and healing.

"Epidermolysis bullosa is a truly horrible, debilitating skin disease in which the top layer of skin is not properly anchored to the underlying layers," said Anthony Oro, MD, PhD, professor of dermatology. "When they are born, the trauma of birth rips away their skin, and they continue to suffer severe skin wounds that require constant bandaging and medical attention throughout their lives."

Stanford has one of the largest epidermolysis bullosa clinics in the world, with an extremely active and engaged population of patients and their families eager to help researchers. The Stanford Department of Dermatology has been working to find new treatments for the disease for over 20 years. The latest advance, in which researchers replaced the mutated, disease-causing gene in the donor-made induced pluripotent stem cells with a healthy version, was funded by an $11.7 million grant from the California Institute for Regenerative Medicine.

New avenue of treatment

"This treatment approach represents an entirely new paradigm for this disease," Oro said. "Normally, treatment has been confined to surgical approaches to repair damaged skin, or medical approaches to prevent and repair damage. But by replacing the faulty gene with a correct version in stem cells, and then converting those corrected stem cells to keratinocytes, we have the possibility of achieving a permanent fix -- replacing damaged areas with healthy, perfectly matched skin grafts."

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Blistering skin disease may be treatable with 'therapeutic reprogramming,' researchers say

November Tip Sheet from Cedars-Sinai Medical Center

By Stem Cell Medical Center

Contact Information

Available for logged-in reporters only

Newswise Following is the November 2014 tip sheet of story ideas from Cedars-Sinai Medical Center. To arrange interviews, please contact the individual listed.

Familys Battle With Cancer Draws Nurse Into Run for Her Brenda Durand, RN, followed in the footsteps of her mother, Bonnie, and became a nurse. But the two women had more in common than their careers. In 2004, Bonnie was diagnosed with stage 3 ovarian cancer. Two years later, Brendas sister learned she had breast cancer, prompting the mother and her two daughters to undergo genetic testing. The results came back: All three women carried the BRCA 1 mutation, putting them at greater risk for breast and ovarian cancer. For the next six years, Brenda and her mom worked to raise funds for ovarian cancer research, with Brenda participating in Cedars-Sinais annual Run for Her 5K Run and Friendship Walk. In 2012, Bonnie lost her life to ovarian cancer. But the loss of her mother has strengthened Brendas resolve to fight harder against ovarian cancer, and on Nov. 9, she participated in the 10th annual Run for Her. She is available for interviews. CONTACT: Cara Martinez, 310-423-7798; Email cara.martinez@cshs.org

Lou Gehrigs Disease Study: Renewing Brains Aging Support Cells May Help Neurons Survive Lou Gehrigs disease, also known as amyotrophic lateral sclerosis, or ALS, attacks muscle-controlling nerve cells motor neurons in the brain, brainstem and spinal cord, leading to progressive weakness and eventual paralysis of muscles throughout the body. Patients typically survive only three to five years after diagnosis. Now, with publication of a study by investigators at the Cedars-Sinai Board of Governors Regenerative Medicine Institute, ALS researchers know the effects of the attack are worsened, at least in part, by the aging and failure of support cells called astrocytes, which normally provide nutrients, housekeeping, structure and other forms of assistance for neurons. CONTACT: Sandy Van, 808-526-1708; Email sandy@prpacific.com

Cardiac Stem Cell Therapy May Heal Heart Damage Caused by Duchenne Muscular Dystrophy Researchers at the Cedars-Sinai Heart Institute have found that injections of cardiac stem cells might help reverse heart damage caused by Duchenne muscular dystrophy, potentially resulting in a longer life expectancy for patients with the chronic muscle-wasting disease. CONTACT: Sally Stewart, 310-248-6566; Email sally.stewart@cshs.org

Cedars-Sinai Study of Lou Gehrigs Disease Shifts Origin Focus to Brains Motor Neurons Lou Gehrigs disease, also known as amyotrophic lateral sclerosis, or ALS, might damage muscle-controlling nerve cells in the brain earlier in the disease process than previously known, according to research from the Cedars-Sinai Board of Governors Regenerative Medicine Institute. The findings could shift researchers attention from the spinal cord to the brains motor cortex as the diseases initial point of dysfunction. CONTACT: Sandy Van, 808-526-1708; Email sandy@prpacific.com

New Alzheimers Program to Focus on Prevention, Intervention, Research and Support Seeking to stem the rapid increase of Alzheimers disease, Cedars-Sinai has launched a new Alzheimers Prevention Program to help identify patients at risk of developing the neurological disorder and to reduce the impact on those diagnosed with the slow-moving condition. The program represents a concerted effort by clinicians, researchers, patients, families, caregivers and community agencies to address an approaching tsunami of Alzheimers care. Medical authorities expect the number of cases nationally to triple by 2050, inundating the healthcare system with patients and costing more than $1 trillion. CONTACT: Sandy Van, 808-526-1708; Email sandy@prpacific.com

Sister to Sister, Nations First Organization Dedicated to Womens Heart Health, Donates Educational Content, Intellectual Property to Cedars-Sinai Heart Institute

After 15 years of educating women about heart disease and providing more than 100,000 free cardiovascular screenings, Sister to Sister: The Womens Heart Health Foundation founded by Irene Pollin, MSW, announced today that the organization will cease operations on Dec. 31. Pollin also announced that the pioneering organization is donating its educational content and intellectual property to the Barbra Streisand Womens Heart Center in the Cedars-Sinai Heart Institute. CONTACT: Sally Stewart, 310-248-6566; Email sally.stewart@cshs.org

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November Tip Sheet from Cedars-Sinai Medical Center

The Miami Stem Cell Treatment Center Announces Adult Stem Cell Public Seminars in The Villages, Florida

By Stem Cell Treatment

The Villages, Florida (PRWEB) November 26, 2014

The Miami Stem Cell Treatment Center announces a series of free public seminars on the use of adult stem cells for various chronic, degenerative and inflammatory conditions. They will be provided by Dr. Thomas A. Gionis, Surgeon-in-Chief and Dr. Nia Smyrniotis, Medical Director.

The seminars will be held on Monday, December 8, 2014, at 1:00pm, 3:00pm and 5:00pm at TownPlace Suites The Villages Marriott, 1141 Alonzo Ave., The Villages, FL 32159. Please RSVP at (561) 331-2999.

The Miami Stem Cell Treatment Center abides by investigational protocols using adult adipose derived stem cells (ADSCs) which can be deployed to improve patients quality of life for a number of chronic, degenerative and inflammatory conditions and diseases. ADSCs are taken from the patients own adipose (fat) tissue (found within a cellular mixture called stromal vascular fraction (SVF)). ADSCs are exceptionally abundant in adipose tissue. The adipose tissue is obtained from the patient during a 15 minute mini-liposuction performed under local anesthesia in the doctors office. SVF is a protein-rich solution containing mononuclear cell lines (predominantly adult autologous mesenchymal stem cells), macrophage cells, endothelial cells, red blood cells, and important Growth Factors that facilitate the stem cell process and promote their activity.

ADSCs are the body's natural healing cells - they are recruited by chemical signals emitted by damaged tissues to repair and regenerate the bodys injured cells. The Miami Stem Cell Treatment Center only uses Adult Autologous Stem Cells from a persons own fat No embryonic stem cells are used. Current areas of study include: Emphysema, COPD, Asthma, Heart Failure, Parkinsons Disease, Stroke, Multiple Sclerosis, Lupus, Rheumatoid Arthritis, Crohns Disease, and degenerative orthopedic joint conditions. For more information, or if someone thinks they may be a candidate for one of the adult stem cell protocols offered by the Miami Stem Cell Treatment Center, they may contact Dr. Smyrniotis or Dr. Gionis directly at (561) 331-2999, or see a complete list of the Centers study areas at: http://www.MiamiStemCellsUSA.com.

About the Miami Stem Cell Treatment Center: The Miami Stem Cell Treatment Center is an affiliate of the Cell Surgical Network (CSN); they are located in Baco Raton, Orlando, and Miami, Florida. We provide care for people suffering from diseases that may be alleviated by access to adult stem cell based regenerative treatment. We utilize a fat transfer surgical technology to isolate and implant the patients own stem cells from a small quantity of fat harvested by a mini-liposuction on the same day. The investigational protocols utilized by the Miami Stem Cell Treatment Center have been reviewed and approved by an IRB (Institutional Review Board) which is registered with the U.S. Department of Health, Office of Human Research Protection; and the study is registered with Clinicaltrials.gov, a service of the U.S. National Institutes of Health (NIH). For more information visit our website: http://www.MiamiStemCellsUSA.com.

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The Miami Stem Cell Treatment Center Announces Adult Stem Cell Public Seminars in The Villages, Florida

Devon Still hurried to be by daughter Leahs side as she got stem-cell treatment

By Stem Cell Treatment

Bengals defensive lineman Devon Still is a busy man these days, practicing and playing football in Cincinnati, then flying over to Philadelphia, where his daughter Leah is a cancer patient at Childrens Hospital.Of course, Still would be the first to tell that what hes going through is nothing compared to his daughters ordeal.

Leah was scheduled to undergo stem-cell treatment Tuesday, in order to alleviate some of the internal damage caused by chemotherapy and radiation. There was no way Still was going to miss out on being by thefour-year-olds side.

Mission accomplished, Still had to hurry back to work.

Support for Leah and other kids stricken with pediatric cancer continues to pour in. On Saturday, members of the AHLs Syracuse Crunchdonned Stills No. 75 Bengals jersey as they competed in theFrozen Dome Classic at the Carrier Dome. According to cincinnati.com, the jerseys were then signed and will be auctioned off, with the proceeds going to Ronald McDonald House.

This former editor and part-time writer at The Post is now happy to prove that if you combine 'blowhard' and 'blaggard,' you get 'blogger.' He previously had used 'Desmond Bieler' as his byline, but feels that shortening the first name to 'Des' nicely conveys his ever-decreasing gravitas. He also covers Fantasy Football.

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Devon Still hurried to be by daughter Leahs side as she got stem-cell treatment

Great Ormond Street stem cell freezing problem may have led to girls death

By Stem Cell Treatment

Technical failures at Great Ormond Street hospital may have contributed to Sophie Ryan-Palmer's death. Photograph: Steve Parsons/PA Archive

A 12-year-old cancer patient may have died because of problems in a stem cell freezing process used by Great Ormond Street hospital.

Technical failures at the childrens NHS trust could have contributed to the death of Sophie Ryan-Palmer, from Sunbury in Surrey, St Pancras coroners court has been told.

In a narrative verdict, the coroner, Mary Hassell, said it was unclear what effect the treatment had but she ruled that the deaths of three other children at the hospital were not connected to difficulties with the medical procedure.

The other three children were 13-month-old Ryan Loughran, from Bournemouth, who died in July, four-year-old Katie Joyce, from Hertfordshire, who died in October, and five-year-old Muhanna al-Hayany, who had come from Kuwait to receive the treatment and died in August this year.

Responding to the findings, a spokesperson for Great Ormond Street said: This has been an immensely distressing process for all of the families involved. These four young patients were extremely poorly children with complex conditions, and it is frustrating for everyone concerned, especially their families, for it still to be unclear exactly what caused the freezing problem and to what extent this might have contributed to one patients eventual outcome.

As soon as we identified a potential problem with our stem cell freezing process in 2013, we stopped freezing cells on site and used alternative facilities in other London hospitals while an investigation was undertaken.

We had tested all of these cells prior to transplant, following UK national standards of testing, and the results of these tests had indicated the cells were alive and viable. Therefore at this stage there was no indication of any problem, and it was only after a period of time had elapsed that the pattern of delayed engraftment began to emerge among a group of patients.

The hospital has reintroduced freezing of cells using an alternative method, which is in use in other hospitals across the country. Great Ormond Streets previous freezing method is in use at other hospitals.

The trusts statement added: We welcome the coroners recommendation to create a more standardised approach to the way the medical community shares knowledge nationally about autologous stem cell transplants in children with cancer, to raise awareness of any issues uncovered and ensure a similar problem does not occur again. Autologous transplants are those where the donor is also the recipient.

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Great Ormond Street stem cell freezing problem may have led to girls death

American Spine Launches Plasma Rich Platelet Therapy and Stem Cell Therapy Treatments

By Stem Cell Medicine

Frederick, Maryland (PRWEB) November 25, 2014

American Spine is a practice on the cutting edge, committed to providing the latest, most effective, and innovative treatments for its patients. As part of this philosophy, American Spine joins the excitement surrounding Regenerative Medicine, a modern and progressive field of orthopedics and sports medicine. Regenerative Medicine involves the application of biological therapy treatments that enhance the body's ability to heal itself.

Dr. Sandeep Sherlekar, M.D., an owner and physician at American Spine, comments: "PRP and Stem Cell Treatments are revolutionary regeneration options for now and the future."

The major benefit of PRP and Stem Cell Therapy Treatments is the use of the patient's own whole blood, bone marrow stem cells and adipose stem cells, thus eliminating the risk of rejection or infection from other donors. These treatments are increasing in popularity as an alternative to other more invasive techniques in treating ailments such as: Muscle Tears, Meniscus and ACL Injuries, Tennis and Golf Elbow, Patellofemoral Pain Syndrome, various types of Tendonitis, Plantar Fasciitis, Spine Disease with either Degenerative Disks or Annular Tearing, Osteoarthritis in the joints and many more. They are used as a way to speed healing after other methods of treatment or to aid in the healing of resistant, long lasting injuries.

The blood and/or stem cells are harvested from the patient and are then prepared using state-of-the-art technologies. The preparation of PRP allows for a platelet sample that is 5 times more concentrated with platelets than normal blood. Similarly, the preparation of the patient's stem cells produces a much higher concentration of stem cells, both of which are essential in the healing process. The PRP and/or stem cells are then injected back into the patient's injured or degenerated tissues under guided imaging. The potential complications of such treatments are similar to those associated with regular joint injections.

American Spine stringently follows the FDA guidelines for the clinical use of blood and stem cells. We do not expand, reproduce or grow anything within a culture.

If you are interested in learning more about these treatments as a method to your own healing process or that of your patients, please visit the following link: http://www.americanspinemd.com/blog/platelet-rich-plasma-prp-and-autologous-stem-cell-treatment or call our offices to request additional information about these revolutionary therapies. We are offering a free consultation for new patients who are considering these treatments. The individual patient's treatment protocol will be formulated and discussed during the consultation.

At American Spine, our multidisciplinary team of physicians treat each patient's individual needs. Using the latest techniques and state-of-the-art equipment to diagnose pain, our practitioners determine a plan to meet each patient's unique diagnosis. We have assembled the very best team of healthcare professionals who can most effectively help our patients with a compassionate and personalized approach. We have 10 Maryland locations and one location in Gettysburg, PA to service your individual needs.

Contact Information: American Spine 1050 Key Parkway, Suite 104, Frederick, MD 21702 ph: 240-629-3939 http://www.americanspinemd.com

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American Spine Launches Plasma Rich Platelet Therapy and Stem Cell Therapy Treatments

UCLA Researchers Identify Protein Key To The Development Of Blood Stem Cells

By Uncategorized

November 25, 2014

Provided by Peter Bracke, UCLA

Understanding the self-replication mechanisms is critical for improving stem cell therapies for blood-related diseases and cancers

Led by Dr. Hanna Mikkola, a member of the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, UCLA scientists have discovered a protein that is integral to the self-replication of hematopoietic stem cells during human development.

The discovery lays the groundwork for researchers to generate hematopoietic stem cells in the lab that better mirror those that develop in their natural environment. This could in turn lead to improved therapies for blood-related diseases and cancers by enabling the creation of patient-specific blood stem cells for transplantation.

The findings are reported online ahead of print in the journal Cell Stem Cell.

Researchers have long been stymied in their efforts to make cell-based therapies for blood and immune diseases more broadly available, because of an inability to generate and expand human hematopoietic stem cells (HSCs) in lab cultures. They have sought to harness the promise of pluripotent stem cells (PSCs), which can transform into almost any cell in the human body, to overcome this roadblock. HSCs are the blood-forming cells that serve as the critical link between PSCs and fully differentiated cells of the blood system. The ability of HSCs to self-renew (replicate themselves) and differentiate to all blood cell types, is determined in part by the environment that the stem cell came from, called the niche.

In the five-year study, Mikkola, Dr. Sacha Prashad and Dr. Vincenzo Calvanese, members of Mikkolas lab and lead authors of the study, investigated a HSC surface protein called GPI-80. They found that it was produced by a specific subpopulation of human fetal hematopoietic cells that were the only group that could self-renew and differentiate into various blood cell types. They also found that this subpopulation of hematopoietic cells was the sole population able to permanently integrate into and thrive within the blood system of a recipient mouse.

Mikkola and colleagues further discovered that GPI-80 identifies HSCs during multiple phases of human HSC development and migration. These include the early first trimester of fetal development when newly generated human hematopoietic stem cells can be found in the placenta, and the second trimester when HSCs are actively replicating in the fetal liver and the fetal bone marrow.

We found that whatever HSC niche we investigated, we could use GPI-80 as the best determinant to find the stem cell as it was being generated or colonized different hematopoietic tissues, said Mikkola, associate professor of molecular, cell and development biology at UCLA and also a member of the Jonsson Comprehensive Cancer Center. Moreover, loss of GPI-80 caused the stem cells to differentiate into mature blood cells rather than HSCs. This essentially tells us that GPI-80 must be present to make HSCs. We now have a very unique marker for investigating how human hematopoietic cells develop, migrate and function.

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UCLA Researchers Identify Protein Key To The Development Of Blood Stem Cells

genucel – Intensive New Stem Cell Eye Therapy Treatment …

By Uncategorized

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genucel - Intensive New Stem Cell Eye Therapy Treatment ...

Leah Still to undergo stem cell therapy

By Stem Cell Treatment

CINCINNATI -- The daughter of a Cincinnati Bengal who has already been through so much has another big day ahead of her.

Leah Still -- Devon Stills daughter -- will undergo a stem cell transplant procedure on Tuesday. The stem cell treatment is an effort to regenerate her bone marrow and stem cells.

Still flew to Philadelphia Monday to be with Leah. They went shopping at a mall.

The smile you have after shutting down the mall, literally. This girl had security and the... http://t.co/HHWtLhf4pf pic.twitter.com/QFRMJsdlCX

Still tweeted another photo Tuesday while they waited for her treatment to begin.

Selfies in the hospital to pass time by as we wait for the stem cells http://t.co/q6JZOIyi9q pic.twitter.com/ogB0J0Gitg

Leah was diagnosed with stage 4 neuroblastoma in June. She had surgery to remove a tumor from her abdomen in September, followed by chemotherapy to try to remove the cancer from her bone marrow.

She has already been treated with a round of chemotherapy and radiation.

Devon Still said the family hopes that will be her only round of chemo and radiation but that it depends on how her results come back. He said it will take four to six weeks to determine if more treatments are necessary.

Follow Devon Still's updates on Twitter at @Dev_Still71

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Leah Still to undergo stem cell therapy

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