Stem Cell Clinic

Wisconsin Scientists Find Genetic Recipe To Turn Stem Cells To Blood

By Stem Cell Medicine

University of Wisconsin-Madison

The ability to reliably and safely make in the laboratory all of the different types of cells in human blood is one key step closer to reality.

Writing today in the journal Nature Communications, a group led by University of Wisconsin-Madison stem cell researcher Igor Slukvin reports the discovery of two genetic programs responsible for taking blank-slate stem cells and turning them into both red and the array of white cells that make up human blood.

[ Watch the Video: What Are Stem Cells? ]

The research is important because it identifies how nature itself makes blood products at the earliest stages of development. The discovery gives scientists the tools to make the cells themselves, investigate how blood cells develop and produce clinically relevant blood products.

This is the first demonstration of the production of different kinds of cells from human pluripotent stem cells using transcription factors, explains Slukvin, referencing the proteins that bind to DNA and control the flow of genetic information, which ultimately determines the developmental fate of undifferentiated stem cells.

During development, blood cells emerge in the aorta, a major blood vessel in the embryo. There, blood cells, including hematopoietic stem cells, are generated by budding from a unique population of what scientists call hemogenic endothelial cells. The new report identifies two distinct groups of transcription factors that can directly convert human stem cells into the hemogenic endothelial cells, which subsequently develop into various types of blood cells.

The factors identified by Slukvins group were capable of making the range of human blood cells, including white blood cells, red blood cells and megakaryocytes, commonly used blood products.

By overexpressing just two transcription factors, we can, in the laboratory dish, reproduce the sequence of events we see in the embryo where blood is made, says Slukvin of the Department of Pathology and Laboratory Medicine in the UW School of Medicine and Public Health and the Wisconsin National Primate Research Center.

The method developed by Slukvins group was shown to produce blood cells in abundance. For every million stem cells, the researchers were able to produce 30 million blood cells.

Visit link:
Wisconsin Scientists Find Genetic Recipe To Turn Stem Cells To Blood

Can heart damage be fixed?

By Stem Cell Medical Center

STORY HIGHLIGHTS

(CNN) -- In medical school, Gerald Karpman was taught that when it comes to matters of the heart, what's done is done.

"If you survived the heart attack, you survived at the level that you were going to be," he recalls. "Whatever damage was done was permanent."

That thinking has prevailed until very recently, when studies involving a handful of patients showed an infusion of stem cells might help rebuild healthy hearts in heart attack survivors.

On March 7, Karpman joined that perilous club. A dermatologist in Camarillo, California, and a former marathon runner, the 66-year-old had a rigorous routine: eight to 10 miles of walking each day and a meticulous, meatless diet.

But that morning, sitting at his home computer, a pain kicked in.

"Within about 30 seconds, I was in extreme discomfort," recalls Karpman, who says it was worse than the kidney stones he once suffered. "I couldn't sit still. I mean even driving the car (to the hospital), I couldn't put a seat belt on; I'm just moving around, just trying to think of something else."

Karpman made it to Los Robles Hospital and Medical Center in Thousand Oaks, where doctors used stents to reopen an artery in his heart and save his life.

As he lay recovering, he took in some grim news: Nearly 20% of his heart muscle was dead, starved of oxygen. Dead heart tissue leaves a scar, interrupting the coordinated muscle action that makes the heart such an efficient pump.

A standard measure of the heart's pumping ability is the ejection fraction, the percentage of blood in the left ventricle that is pumped out with each heartbeat. A healthy ejection fraction is between 55 and 70, according to the American Heart Association. Karpman's was 30.

Continued here:
Can heart damage be fixed?

Parkinson's stem cell effort holds fundraiser

By Stem Cell Treatment

Attendees at a fundraiser for Parkinson's patients enjoy food and the setting sun at the home of Jeffrey Strauss, owner of the Pamplemousse Grill. The proceeds help Summit4StemCell.org.

A bold experiment to relieve Parkinson's disease symptoms for many years faces a Nov. 4 deadline to raise a total of $2.5 million. That money will allow the group running the project to get matching funds from California's stem cell agency.

Thanks to the owner/chef of Pamplemousse Grill and a number of donors, the group just took a giant step toward that goal.

The group, Summit4StemCell.org, has been holding events for years to raise money to research the therapy, which will use skin cells from eight patients to form new brain cells. The cells will be implanted in the patients' brains to replace the cells destroyed in Parkinson's that make the neurotransmitter dopamine.

Supporters and some patients have climbed atop Mt. Kilimanjaro in Africa and to Base Camp at Mt. Everest, as well as holding local fundraisers. That money has advanced the research so it's feasible to try it in the patients. But more money is needed to pay for the treatment and related expenses.

So last week, supporters gathered at the home of Jeffrey Strauss, owner of Pamplemousse Grille in Solana Beach.

Proceeds from the $500-a-plate dinner brought in nearly $1 million for the project, said Sherrie Gould, an organizer and project sparkplug. Gould, a nurse practitioner at Scripps Clinic, interfaces between the clinical side, led by neurologist Melissa Houser of Scripps Clinic, and the research side, led by Jeanne Loring, a stem cell scientist at The Scripps Research Institute, and Andres Bratt-Leal of the Parkinsons Association of San Diego. The association is the nonprofit under which Summit4StemCell is held.

"Jeffrey is very close to a couple of our patients, and he literally opened up his home to about 130 people, completely provided food, drink, open bar, wine, wait staff, linens..." Gould said.

Counting in-kind donations, the total raised so far is about $1.5 million, meaning that Summit4StemCell needs to raise another $1 million by September, for a total of $2.5 million, Gould said. With that amount, the group can apply for $2.5 million in matching funds from the California Institute for Regenerative Medicine.

"Funding our mission has been at a grass-roots level, philanthropy, and a few minor grants," the project said in a fundraising email. "With over 900 people donating to the project we have been able to successfully biopsy the patients skin and create dopamine-producing neurons from eight of Dr. Houser's PD patients. We are now comparing cell lines with other researchers' lines, characterizing/purifying the cells, and most exciting: testing the neurons from our patients in an animal model of PD."

See original here:
Parkinson's stem cell effort holds fundraiser

The possible alternatives to bone marrow transplant

By Stem Cell Doctors

;

AP Photo/Agapito Sanchez, Baylor College of Medicine

MONTREAL Finding a donor for a stem cell transplant is perhaps one of the most difficult things for a cancer patient.

This is because stem cells are one of the few things that patients cannot rely on their immediate family to donate, according to to Doctor Silvy Lachance, Director of the stem cell transplant program at Hpital Maisonneuve-Rosemont.

Of course, we first look within the family, she said.

But there is only 25 per cent chance of identifying a donor. If we dont find a donor within the family, we try the international donor registry.

According to the National Cancer Institute, bone marrow and peripheral blood stem cell transplantations are most commonly used to treat leukemia, lymphoma, neuroblastoma (a cancer that affects mostly infants and children) and multiple myeloma.

While they wait for a compatible donor, patients will be assigned a conditioning regiment, which may include radiation.

This conditioning regiment will be followed by the infusion of stem cells that are compatible with the recipient, said Lachance.

Yet, for most ethnic minorities or anyone of mixed-birth, the chances of finding an anonymous donor remain very difficult.

Read this article:
The possible alternatives to bone marrow transplant

Can stem cell treatment reverse heart damage?

By Stem Cell Treatment

In medical school, Gerald Karpman was taught that when it comes to matters of the heart, what's done is done.

"If you survived the heart attack, you survived at the level that you were going to be," he recalls. "Whatever damage was done was permanent."

That thinking has prevailed until very recently, when studies involving a handful of patients showed an infusion of stem cells might help rebuild healthy hearts in heart attack survivors.

On March 7, Karpman joined that perilous club. A dermatologist in Camarillo, California, and a former marathon runner, the 66-year-old had a rigorous routine: eight to 10 miles of walking each day and a meticulous, meatless diet.

But that morning, sitting at his home computer, a pain kicked in.

"Within about 30 seconds, I was in extreme discomfort," recalls Karpman, who says it was worse than the kidney stones he once suffered. "I couldn't sit still. I mean even driving the car (to the hospital), I couldn't put a seat belt on; I'm just moving around, just trying to think of something else."

Karpman made it to Los Robles Hospital and Medical Center in Thousand Oaks, where doctors used stents to reopen an artery in his heart and save his life.

As he lay recovering, he took in some grim news: Nearly 20 percent of his heart muscle was dead, starved of oxygen. Dead heart tissue leaves a scar, interrupting the coordinated muscle action that makes the heart such an efficient pump.

A standard measure of the heart's pumping ability is the ejection fraction, the percentage of blood in the left ventricle that is pumped out with each heartbeat. A healthy ejection fraction is between 55 and 70, according to the American Heart Association. Karpman's was 30.

Damage as severe as what Karpman suffered carries a high risk of developing heart failure.

Here is the original post:
Can stem cell treatment reverse heart damage?

Heart attack damage may be reversible

By Stem Cell Medical Center

STORY HIGHLIGHTS

For more, watch "Sanjay Gupta | M.D." on Saturday at 4:30 p.m. and Sunday at 7:30 a.m. ET.

(CNN) -- In medical school, Gerald Karpman was taught that when it comes to matters of the heart, what's done is done.

"If you survived the heart attack, you survived at the level that you were going to be," he recalls. "Whatever damage was done was permanent."

That thinking has prevailed until very recently, when studies involving a handful of patients showed an infusion of stem cells might help rebuild healthy hearts in heart attack survivors.

On March 7, Karpman joined that perilous club. A dermatologist in Camarillo, California, and a former marathon runner, the 66-year-old had a rigorous routine: eight to 10 miles of walking each day and a meticulous, meatless diet.

But that morning, sitting at his home computer, a pain kicked in.

"Within about 30 seconds, I was in extreme discomfort," recalls Karpman, who says it was worse than the kidney stones he once suffered. "I couldn't sit still. I mean even driving the car (to the hospital), I couldn't put a seat belt on; I'm just moving around, just trying to think of something else."

Karpman made it to Los Robles Hospital and Medical Center in Thousand Oaks, where doctors used stents to reopen an artery in his heart and save his life.

As he lay recovering, he took in some grim news: Nearly 20% of his heart muscle was dead, starved of oxygen. Dead heart tissue leaves a scar, interrupting the coordinated muscle action that makes the heart such an efficient pump.

Read the original here:
Heart attack damage may be reversible

How safe is stem cell therapy for children affected with autism spectrum disorder – Video

By Uncategorized


How safe is stem cell therapy for children affected with autism spectrum disorder
In conversation with Dr Alok Sharma (MS, MCh.) Professor of Neurosurgery Head of Department, LTMG Hospital LTM Medical College, Sion, Mumbai. Explains, How safe is stem cell therapy for...

By: Neurogen Brain and Spine Institute

Originally posted here:
How safe is stem cell therapy for children affected with autism spectrum disorder - Video

Saved from amputation – how a stem cell gel rebuilt my shattered leg

By Stem Cell Treatment

Clive Randell, 57, injured his leg in a motorcycle accident in 2011 Thanks to a new stem cell procedure, he can now ride his bike again Stem cells taken from the pelvis are blended with gel to 'glue' the bone

By David Gerrie

Published: 16:01 EST, 12 July 2014 | Updated: 02:33 EST, 13 July 2014

A pioneering stem cell procedure to repair fractured bones could provide a lifeline for accident victims facing the amputation of a limb.

The development involves harvesting stem cells master cells that are able to transform into any kind of body tissue from the patients pelvis, blending them with a specially created gel and injecting the solution into the damaged bone.

One patient already benefiting is lifelong motorcycle enthusiast Clive Randell who suffered horrific injuries to his left leg when his Harley-Davidson was rammed by a car in 2011.

On yer bike: Clive Randell, 57, pictured with his 'saviour' Professor Anan Shetty at Kents Canterbury Christ Church University, can now ride his bike again after undergoing the new stem cell procedure

He suffered multiple open fractures, leaving bone protruding through the skin, and extensive skin loss. Doctors repeatedly told him his leg would have to be amputated.

Today, though, Clive, 57, is back on his feet and, astonishingly, also his bike thanks to the ground-breaking stem-cell treatment.

Follow this link:
Saved from amputation - how a stem cell gel rebuilt my shattered leg

California stem cell agency to review dealings with StemCells Inc.

By Stem Cell Medicine

Californias stem cell agency announced Wednesday it was taking steps to deal with a risk of conflict of interest two days after its former president took a position with a company that the state agency had been funding.

Newark, Calif.-based StemCells Inc. announced that Alan Trounson would be joining its board of directors a week after Trounson left the top spot at the California Institute for Regenerative Medicine, according to California Stem Cell Report, a blog that follows developments related to the state agency.

The movehas an appearance of a serious conflict of interest, said John M. Simpson, director of the stem cell project for Santa Monica-based nonprofit Consumer Watchdog, in an interview.

The thing smacks as if this is StemCells Inc. giving a payback to Trounson after the agency awarded $19 million to StemCells Inc. Thats just the way it looks, Simpson said. And I think they have to explain quite clearly why that is not the case.

In a statement, the state stem cell agency said it had not known of the move and learned of Trounson's new position from a press release.

We take even the appearance of conflicts of interest very seriously, the agencys current president, C. Randal Mills, said in a statement, adding that they would be taking actions to address mounting concerns. We understand that the appointment of CIRMs former president to the board of directors of a CIRM loan recipient creates a risk of a conflict of interest.

Under current law, it is illegal for Trounson to communicate with the stem cell agencys board members or employees on behalf of his company to further its interests. The restriction lasts one year; Simpson argued that such a restriction should last for two years.

The agency has also banned its employees and its governing board from communicating with Trounson about any matter regarding StemCells Inc., and will be reviewing all of its work relating to the company, which the state agency says is receiving funding for Alzheimers research.

Simpson praised the agencys actions, but pointed out that conflict of interest has been a recurrent issue between the state agency and the organizations it funds. The stem cell agency was created by Proposition 71 in 2004 to distribute $3 billion of taxpayer funds to companies and institutions performing stem cell research.

With some 90% of the agency's grants having gone to institutions with representatives on its board, Times columnist Michael Hiltzik wrote in a 2012 story, the agency has long been vulnerable to charges of conflicts of interest.

More:
California stem cell agency to review dealings with StemCells Inc.

Conflicts of interest at stem-cell agency yet again

By Stem Cell Medicine

Here we go again. In the decade since California voters established a one-of-a-kind state stem-cell research agency with $3 billion in bond funding, the agency has been in the news over and over because of conflicts of interests.

Members of the board governing the California Institute for Regenerative Medicine often are employed by institutions seeking board grants. While these members cant vote directly to give grants to their employers, the incentive for mutually beneficial voting is obvious. Thats why ethical complaints began almost as soon as the first grants were made.

Last year, finally, the institute added some strong new safeguards. Its key members then made the rounds at California newspapers to argue that it was time for the medias focus to shift to all the promising work that agency grants had yielded. Their argument seemed reasonable.

But this week, the U-T reported that recently resigned institute President Alan Trounson is joining the board of StemCells Inc. which got $19.4 million in grants from the agency.

Trounson hasnt broken any law, and agency officials are properly critical of his decision. But this still makes the stem-cell institute look shabby and the timing could hardly be worse. The original $3 billion in funding will be used up within three years.

Whether the institute seeks additional money from voters or the Legislature, its unsavory history will be hard to overcome.

Read more from the original source:
Conflicts of interest at stem-cell agency yet again

→←